Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8UEM4 (NRDJ_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vitamin B12-dependent ribonucleotide reductase

EC=1.17.4.1
Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ
Gene names
Name:nrdJ
Ordered Locus Names:Atu1733
ORF Names:AGR_C_3183
OrganismAgrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]
Taxonomic identifier176299 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

Protein attributes

Sequence length1272 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

5'-deoxyadenosylcobalamine (coenzyme B12) By similarity.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase class-2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12721272Vitamin B12-dependent ribonucleotide reductase
PRO_0000231657

Regions

Region198 – 1992Substrate binding By similarity
Region474 – 4785Substrate binding By similarity
Region675 – 6795Substrate binding By similarity

Sites

Active site4741Proton acceptor By similarity
Active site4761Cysteine radical intermediate By similarity
Active site4781Proton acceptor By similarity
Binding site1531Substrate By similarity
Binding site2301Substrate; via amide nitrogen By similarity

Amino acid modifications

Disulfide bond199 ↔ 487Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8UEM4 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 3FA22DCC85AC6E2D

FASTA1,272139,623
        10         20         30         40         50         60 
MRIERRFTKP GQSSYAEIEF RKAVSEIKNP DGSVVFRLAD IDVPAQFSQV ATDVLAQKYF 

        70         80         90        100        110        120 
RKAGVPKLLK KVEENDVPSF LWRSVADEKA LKELPEAERY GSETDARQVF DRLAGTWAYW 

       130        140        150        160        170        180 
GWKGKYFSTE EDASAFKDEL AYMLATQRVA PNSPQWFNTG LHWAYGIDGP GQGHFYVDPF 

       190        200        210        220        230        240 
TGKLTKSKSA YEHPQPHACF IQSVEDDLVN EGGIMDLWVR EARLFKYGSG TGSNFSYLRG 

       250        260        270        280        290        300 
EGEKLSGGGK SSGLMSFLKI GDRAAGAIKS GGTTRRAAKM VVVDADHPDI EAYIDWKVNE 

       310        320        330        340        350        360 
EQKVAALVTG SKIVAKHLKA IMKACVNCEA DNGDCFDPAK NPALKREIRA AKKDMVPENY 

       370        380        390        400        410        420 
VKRVIQFAEQ GYKDIQFKTY DTDWDSEAYL TVSGQNSNNS VSLKDDFLRA VENDGNWNLT 

       430        440        450        460        470        480 
ARKDGKVMKT LKARDLWEKI SHAAWASADP GLHFNTTMND WHTSPAEGPI RASNPCSEYM 

       490        500        510        520        530        540 
FLDDTACNLA SLNLLQFKDA KTKRIDIADY EHAVRLWTVV LEVSVMMAQF PSRQIAERSY 

       550        560        570        580        590        600 
EYRTLGLGYA NIGGLLMSSG IPYDSDEGRA IAGALTAIMT GVSYATSAEM AGELGPFPSF 

       610        620        630        640        650        660 
APNRDNMLRV IRNHRRAAHG QSEGYEGLSV NPVALIHADC TDQDLVAHAT AAWDKALELG 

       670        680        690        700        710        720 
EKHGYRNAQT TVIAPTGTIG LVMDCDTTGI EPDFALVKFK KLAGGGYFKI INRAVPESLR 

       730        740        750        760        770        780 
SLGYSESQIA EIEAYAVGHG NLNQAPAINP STLKAKGFTD EKIEAVNAAL KSAFDIKFVF 

       790        800        810        820        830        840 
NQWTLGADFL KGTLKVSDEQ LSDMSFNLLD HLGFAKKDIE AANVHVCGAM TLEGAPFLKN 

       850        860        870        880        890        900 
EHLAVFDCAN PCGKIGKRYL SVESHIRMMA AAQPFISGAI SKTINMPNDA TVEDCGAAYM 

       910        920        930        940        950        960 
LSWKLALKAN ALYRDGSKLS QPLNASLVED EDDEDFVEEL IQQPLAQQAV TITEKIVERV 

       970        980        990       1000       1010       1020 
IERVSREREK LPNRRQGYTQ KATVGGHKVY LRTGEFGDGR IGEIFIDMHK EGAAFRAMMN 

      1030       1040       1050       1060       1070       1080 
NFAIAISLGL QYGVPLEEYV EAFTFTKFEP AGMVQGNDAI KNATSILDYV FRELAVSYLG 

      1090       1100       1110       1120       1130       1140 
RHDLAHVDTS DFSNTALGKG IQEGKTNLLS TGWTRGYKPT LVSSNEGDRA ASEPKGSATA 

      1150       1160       1170       1180       1190       1200 
APARGSANVT SFAGSAARKL EPTVAITTSE IVSFKRDYEE RAKELAEEIA EEVIDEVVQE 

      1210       1220       1230       1240       1250       1260 
AQQTATALFS DKAAADAASA KAEAKKKENE RRMRSIAQGY TGNMCSECQN FTMVRNGTCE 

      1270 
KCDTCGATSG CS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE007869 Genomic DNA. Translation: AAK87503.1.
PIRAF2789.
F97568.
RefSeqNP_354718.1. NC_003062.2.

3D structure databases

ProteinModelPortalQ8UEM4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176299.Atu1733.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK87503; AAK87503; Atu1733.
GeneID1139199.
KEGGatu:Atu1733.
PATRIC20813235. VBIAgrTum91616_1745.

Phylogenomic databases

eggNOGCOG0209.
HOGENOMHOG000021770.
KOK00525.
OMATAFDIKF.
OrthoDBEOG6H4K5F.
ProtClustDBPRK07562.

Enzyme and pathway databases

BioCycAGRO:ATU1733-MONOMER.

Family and domain databases

Gene3D1.10.10.200. 1 hit.
InterProIPR017856. Integrase_Zn-bd_dom-like_N.
IPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
[Graphical view]
PfamPF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 2 hits.
PF12637. TSCPD. 1 hit.
[Graphical view]
PRINTSPR01183. RIBORDTASEM1.
TIGRFAMsTIGR02504. NrdJ_Z. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNRDJ_AGRT5
AccessionPrimary (citable) accession number: Q8UEM4
Secondary accession number(s): Q7CYN2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families