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Q8UEM4

- NRDJ_AGRT5

UniProt

Q8UEM4 - NRDJ_AGRT5

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Protein

Vitamin B12-dependent ribonucleotide reductase

Gene
nrdJ, Atu1733, AGR_C_3183
Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

5'-deoxyadenosylcobalamine (coenzyme B12) By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei153 – 1531Substrate By similarity
Binding sitei230 – 2301Substrate; via amide nitrogen By similarity
Active sitei474 – 4741Proton acceptor By similarity
Active sitei476 – 4761Cysteine radical intermediate By similarity
Active sitei478 – 4781Proton acceptor By similarity

GO - Molecular functioni

  1. cobalamin binding Source: UniProtKB-KW
  2. cobalt ion binding Source: InterPro
  3. nucleotide binding Source: UniProtKB-KW
  4. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

Cobalamin, Cobalt, Nucleotide-binding

Enzyme and pathway databases

BioCyciAGRO:ATU1733-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12-dependent ribonucleotide reductase (EC:1.17.4.1)
Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ
Gene namesi
Name:nrdJ
Ordered Locus Names:Atu1733
ORF Names:AGR_C_3183
OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000000813: Chromosome circular

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12721272Vitamin B12-dependent ribonucleotide reductasePRO_0000231657Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi199 ↔ 487Redox-active By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi176299.Atu1733.

Structurei

3D structure databases

ProteinModelPortaliQ8UEM4.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni198 – 1992Substrate binding By similarity
Regioni474 – 4785Substrate binding By similarity
Regioni675 – 6795Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0209.
HOGENOMiHOG000021770.
KOiK00525.
OMAiTAFDIKF.
OrthoDBiEOG6H4K5F.

Family and domain databases

Gene3Di1.10.10.200. 1 hit.
InterProiIPR017856. Integrase_Zn-bd_dom-like_N.
IPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
IPR029072. YebC-like.
[Graphical view]
PfamiPF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 2 hits.
PF12637. TSCPD. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF75625. SSF75625. 1 hit.
TIGRFAMsiTIGR02504. NrdJ_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8UEM4-1 [UniParc]FASTAAdd to Basket

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MRIERRFTKP GQSSYAEIEF RKAVSEIKNP DGSVVFRLAD IDVPAQFSQV     50
ATDVLAQKYF RKAGVPKLLK KVEENDVPSF LWRSVADEKA LKELPEAERY 100
GSETDARQVF DRLAGTWAYW GWKGKYFSTE EDASAFKDEL AYMLATQRVA 150
PNSPQWFNTG LHWAYGIDGP GQGHFYVDPF TGKLTKSKSA YEHPQPHACF 200
IQSVEDDLVN EGGIMDLWVR EARLFKYGSG TGSNFSYLRG EGEKLSGGGK 250
SSGLMSFLKI GDRAAGAIKS GGTTRRAAKM VVVDADHPDI EAYIDWKVNE 300
EQKVAALVTG SKIVAKHLKA IMKACVNCEA DNGDCFDPAK NPALKREIRA 350
AKKDMVPENY VKRVIQFAEQ GYKDIQFKTY DTDWDSEAYL TVSGQNSNNS 400
VSLKDDFLRA VENDGNWNLT ARKDGKVMKT LKARDLWEKI SHAAWASADP 450
GLHFNTTMND WHTSPAEGPI RASNPCSEYM FLDDTACNLA SLNLLQFKDA 500
KTKRIDIADY EHAVRLWTVV LEVSVMMAQF PSRQIAERSY EYRTLGLGYA 550
NIGGLLMSSG IPYDSDEGRA IAGALTAIMT GVSYATSAEM AGELGPFPSF 600
APNRDNMLRV IRNHRRAAHG QSEGYEGLSV NPVALIHADC TDQDLVAHAT 650
AAWDKALELG EKHGYRNAQT TVIAPTGTIG LVMDCDTTGI EPDFALVKFK 700
KLAGGGYFKI INRAVPESLR SLGYSESQIA EIEAYAVGHG NLNQAPAINP 750
STLKAKGFTD EKIEAVNAAL KSAFDIKFVF NQWTLGADFL KGTLKVSDEQ 800
LSDMSFNLLD HLGFAKKDIE AANVHVCGAM TLEGAPFLKN EHLAVFDCAN 850
PCGKIGKRYL SVESHIRMMA AAQPFISGAI SKTINMPNDA TVEDCGAAYM 900
LSWKLALKAN ALYRDGSKLS QPLNASLVED EDDEDFVEEL IQQPLAQQAV 950
TITEKIVERV IERVSREREK LPNRRQGYTQ KATVGGHKVY LRTGEFGDGR 1000
IGEIFIDMHK EGAAFRAMMN NFAIAISLGL QYGVPLEEYV EAFTFTKFEP 1050
AGMVQGNDAI KNATSILDYV FRELAVSYLG RHDLAHVDTS DFSNTALGKG 1100
IQEGKTNLLS TGWTRGYKPT LVSSNEGDRA ASEPKGSATA APARGSANVT 1150
SFAGSAARKL EPTVAITTSE IVSFKRDYEE RAKELAEEIA EEVIDEVVQE 1200
AQQTATALFS DKAAADAASA KAEAKKKENE RRMRSIAQGY TGNMCSECQN 1250
FTMVRNGTCE KCDTCGATSG CS 1272
Length:1,272
Mass (Da):139,623
Last modified:June 1, 2002 - v1
Checksum:i3FA22DCC85AC6E2D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007869 Genomic DNA. Translation: AAK87503.1.
PIRiAF2789.
F97568.
RefSeqiNP_354718.1. NC_003062.2.

Genome annotation databases

EnsemblBacteriaiAAK87503; AAK87503; Atu1733.
GeneIDi1139199.
KEGGiatu:Atu1733.
PATRICi20813235. VBIAgrTum91616_1745.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007869 Genomic DNA. Translation: AAK87503.1 .
PIRi AF2789.
F97568.
RefSeqi NP_354718.1. NC_003062.2.

3D structure databases

ProteinModelPortali Q8UEM4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 176299.Atu1733.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK87503 ; AAK87503 ; Atu1733 .
GeneIDi 1139199.
KEGGi atu:Atu1733.
PATRICi 20813235. VBIAgrTum91616_1745.

Phylogenomic databases

eggNOGi COG0209.
HOGENOMi HOG000021770.
KOi K00525.
OMAi TAFDIKF.
OrthoDBi EOG6H4K5F.

Enzyme and pathway databases

BioCyci AGRO:ATU1733-MONOMER.

Family and domain databases

Gene3Di 1.10.10.200. 1 hit.
InterProi IPR017856. Integrase_Zn-bd_dom-like_N.
IPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
IPR029072. YebC-like.
[Graphical view ]
Pfami PF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 2 hits.
PF12637. TSCPD. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF75625. SSF75625. 1 hit.
TIGRFAMsi TIGR02504. NrdJ_Z. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.

Entry informationi

Entry nameiNRDJ_AGRT5
AccessioniPrimary (citable) accession number: Q8UEM4
Secondary accession number(s): Q7CYN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi