Reviewed, UniProtKB/Swiss-Prot Q8UEL1 (SYT_AGRT5)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	Atu1746
ORF Names:	AGR_C_3205

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	667 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00184
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 667	667	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_0000100931
Regions
	Region	245 – 553	309	Catalytic HAMAP MF_00184
Sites
	Metal binding	347	1	Zinc; catalytic By similarity
	Metal binding	398	1	Zinc; catalytic By similarity
	Metal binding	530	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UEL1-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 85FC6EA7F1674AD9	FASTA	667	75,629
	10 20 30 40 50 60 MSEAISLTFP DGSVRSYPAG TTGREVAESI SKSLAKKAVA IALDGTVRDL SETITDGKIE 70 80 90 100 110 120 IVTREDGRAL ELIRHDAAHV MAEAVQELWP GTQVTIGPVI ENGFYYDFAK NEPFTPEDLP 130 140 150 160 170 180 KIEKRMKEII QRNKPFTREI WSREKAKEVF AAKGENYKVE LVDAIPEGQD LKIYYQGDWF 190 200 210 220 230 240 DLCRGPHMAS TGQIGTAFKL MKVAGAYWRG DSNNAMLSRI YGTAWATQEE LDNYLHVLAE 250 260 270 280 290 300 AEKRDHRRLG REMDLFHFQE EGPGVVFWHG KGWRMFQTLT AYMRRRLANT YQEVNAPQVL 310 320 330 340 350 360 DKSLWETSGH WGWYQENMFA VKSAHAFTHP DDKEADQRVF ALKPMNCPGH VQIFKHGLKS 370 380 390 400 410 420 YREMPVRLAE FGNVHRYEAS GALHGLMRVR GFTQDDAHVF CTEEQMAAEC LRINDLILSV 430 440 450 460 470 480 YEDFGFSEIV VKLSTRPEKR VGSDDLWDRA ESVMLEVLKT IEEQSGGRIK TGILPGEGAF 490 500 510 520 530 540 YGPKFEYTLK DAIGREWQCG TTQVDFNLPE RFGAFYIDQN SEKTQPVMIH RAICGSMERF 550 560 570 580 590 600 LGILIENFAG HMPLWFAPLQ VVVATITSDA DDYGREVAEA LREAGMAVET DFRNEKINYK 610 620 630 640 650 660 VREHSVTKVP VIIVCGRKEA EERTVNIRRL GSQNQTSMSL EEAITSLVDE ATPPDVKRKL AAKKQLA

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007869 Genomic DNA. Translation: AAK87515.1.
PIR	AC2791. B97570.
RefSeq	NP_354730.1.
3D structure databases
HSSP	HSSP built from PDB template 1EVL based on UniProtKB P00955.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UEL1.
Genome annotation databases
GeneID	1133784.
GenomeReviews	Gene locus Atu1746 in contig AE007869_GR.
KEGG	atc:AGR_C_3205. atu:Atu1746.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8UEL1.
OMA	ELELFMF.
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_AGRT5

Accession

Primary (citable) accession number: Q8UEL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)