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Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

purL

Organism
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Catalytic activityi

ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase subunit PurL (purL), Phosphoribosylformylglycinamidine synthase subunit PurQ (purQ), Phosphoribosylformylglycinamidine synthase subunit PurS (purS)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei81UniRule annotation1
Binding sitei84ATPUniRule annotation1
Binding sitei123ATPUniRule annotation1
Metal bindingi125Magnesium 1UniRule annotation1
Active sitei127Proton acceptorUniRule annotation1
Binding sitei148SubstrateUniRule annotation1
Metal bindingi149Magnesium 2UniRule annotation1
Binding sitei272SubstrateUniRule annotation1
Metal bindingi300Magnesium 2UniRule annotation1
Binding sitei525ATPUniRule annotation1
Binding sitei562ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi563Magnesium 1UniRule annotation1
Binding sitei565SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAGRO:ATU1850-MONOMER
UniPathwayiUPA00074; UER00128

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
Short name:
FGAR amidotransferase IIUniRule annotation
Short name:
FGAR-AT IIUniRule annotation
Glutamine amidotransferase PurLUniRule annotation
Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
Gene namesi
Name:purLUniRule annotation
Ordered Locus Names:Atu1850
ORF Names:AGR_C_3393
OrganismiAgrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
Proteomesi
  • UP000000813 Componenti: Chromosome circular

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001004291 – 775Phosphoribosylformylglycinamidine synthase subunit PurLAdd BLAST775

Interactioni

Subunit structurei

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Protein-protein interaction databases

STRINGi176299.Atu1850

Structurei

3D structure databases

ProteinModelPortaliQ8UEB0
SMRiQ8UEB0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 129Substrate bindingUniRule annotation4
Regioni344 – 346Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the FGAMS family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JIU Bacteria
COG0046 LUCA
HOGENOMiHOG000238227
KOiK01952
OMAiFIEPYQG

Family and domain databases

Gene3Di3.30.1330.10, 2 hits
3.90.650.10, 4 hits
HAMAPiMF_00420 PurL_2, 1 hit
InterProiView protein in InterPro
IPR010074 PRibForGlyAmidine_synth_PurL
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
PANTHERiPTHR43555 PTHR43555, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 2 hits
PF02769 AIRS_C, 2 hits
PIRSFiPIRSF001587 FGAM_synthase_II, 1 hit
SUPFAMiSSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
TIGRFAMsiTIGR01736 FGAM_synth_II, 1 hit

Sequencei

Sequence statusi: Complete.

Q8UEB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVKLRIFLS HGEASCAKEP ETFLGSNRVR PMSISNSIKI TPELVASHGL
60 70 80 90 100
KPDEYQRILD LIGREPSFTE LGIFSAMWNE HCSYKSSKKW LKTLPTTGPR
110 120 130 140 150
VIQGPGENAG VVDIDDGDCV VFKMESHNHP SYIEPYQGAA TGVGGILRDV
160 170 180 190 200
FTMGARPIAA MNALRFGAPD HPKTRHLVAG VVAGVGGYGN SFGVPTVGGE
210 220 230 240 250
VEFDPRYNGN ILVNAFAAGL AKSNAIFLSE AKGVGLPVVY LGAKTGRDGV
260 270 280 290 300
GGATMASAEF DESIEEKRPT VQVGDPFTEK CLLEACLELM KTGAVIAIQD
310 320 330 340 350
MGAAGLTCSA VEMGAKGDLG IELDLNAVPV REERMTAYEM MLSESQERML
360 370 380 390 400
MVLEPSKEEV AKAIFVKWGL DFAIVGKTTD DLRFRVLHNG EEVANLPIKE
410 420 430 440 450
LGDEAPEYDR PWTPAKVPAA LSETDIPEAD IADALVSLVG SANNSSRRWV
460 470 480 490 500
YEQYDTLIQG NSLQLPGGDA GVVRVEGHDK KALAFSSDVT PRYVEADAFE
510 520 530 540 550
GGKQAVAECW RNITATGALP LAATDNLNFG NPEKPEIMSQ LVHAIKGIGE
560 570 580 590 600
ACRVLEFPIV SGNVSLYNET NGQAILPTPT IGGVGLLKDW GRMARIRFAA
610 620 630 640 650
ADEVVLLVGA PAGLGTHIAQ SVYMRDVHGR TDGPAPHVDL IAEKKNGDFV
660 670 680 690 700
RGLITEGLTT AVHDCSSGGL ALAVAEMAIS SGIGATIDAV EGHNPILTFY
710 720 730 740 750
GEDQARYVLT VKKSDLDKVR AAAKAAGVSC PLIGTTGGST VKLGTARAVE
760 770
IKELHLAYES WFPQFMDGET LIAAE
Length:775
Mass (Da):82,517
Last modified:June 1, 2002 - v1
Checksum:i2737EECB6D2699BE
GO

Sequence cautioni

The sequence AAK87617 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA Translation: AAK87617.2 Different initiation.
PIRiAH2803
H97582
RefSeqiNP_354832.2, NC_003062.2

Genome annotation databases

EnsemblBacteriaiAAK87617; AAK87617; Atu1850
GeneIDi1133888
KEGGiatu:Atu1850
PATRICifig|176299.10.peg.1863

Entry informationi

Entry nameiPURL_AGRFC
AccessioniPrimary (citable) accession number: Q8UEB0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health