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Q8UEB0 (PURL_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3
Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II
Gene names
Name:purL
Ordered Locus Names:Atu1850
ORF Names:AGR_C_3393
OrganismAgrobacterium tumefaciens (strain C58 / ATCC 33970)
Taxonomic identifier176299 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length775 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420

Subunit structure

Heterodimer of two subunits, PurQ and PurL.

Subcellular location

Cytoplasm By similarity HAMAP MF_00420.

Sequence similarities

Belongs to the FGAMS family.

Sequence caution

The sequence AAK87617.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 775775Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420
PRO_0000100429

Regions

Nucleotide binding138 – 14912ATP Potential

Sequences

Sequence LengthMass (Da)Tools
Q8UEB0 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 2737EECB6D2699BE

FASTA77582,517
        10         20         30         40         50         60 
MPVKLRIFLS HGEASCAKEP ETFLGSNRVR PMSISNSIKI TPELVASHGL KPDEYQRILD 

        70         80         90        100        110        120 
LIGREPSFTE LGIFSAMWNE HCSYKSSKKW LKTLPTTGPR VIQGPGENAG VVDIDDGDCV 

       130        140        150        160        170        180 
VFKMESHNHP SYIEPYQGAA TGVGGILRDV FTMGARPIAA MNALRFGAPD HPKTRHLVAG 

       190        200        210        220        230        240 
VVAGVGGYGN SFGVPTVGGE VEFDPRYNGN ILVNAFAAGL AKSNAIFLSE AKGVGLPVVY 

       250        260        270        280        290        300 
LGAKTGRDGV GGATMASAEF DESIEEKRPT VQVGDPFTEK CLLEACLELM KTGAVIAIQD 

       310        320        330        340        350        360 
MGAAGLTCSA VEMGAKGDLG IELDLNAVPV REERMTAYEM MLSESQERML MVLEPSKEEV 

       370        380        390        400        410        420 
AKAIFVKWGL DFAIVGKTTD DLRFRVLHNG EEVANLPIKE LGDEAPEYDR PWTPAKVPAA 

       430        440        450        460        470        480 
LSETDIPEAD IADALVSLVG SANNSSRRWV YEQYDTLIQG NSLQLPGGDA GVVRVEGHDK 

       490        500        510        520        530        540 
KALAFSSDVT PRYVEADAFE GGKQAVAECW RNITATGALP LAATDNLNFG NPEKPEIMSQ 

       550        560        570        580        590        600 
LVHAIKGIGE ACRVLEFPIV SGNVSLYNET NGQAILPTPT IGGVGLLKDW GRMARIRFAA 

       610        620        630        640        650        660 
ADEVVLLVGA PAGLGTHIAQ SVYMRDVHGR TDGPAPHVDL IAEKKNGDFV RGLITEGLTT 

       670        680        690        700        710        720 
AVHDCSSGGL ALAVAEMAIS SGIGATIDAV EGHNPILTFY GEDQARYVLT VKKSDLDKVR 

       730        740        750        760        770 
AAAKAAGVSC PLIGTTGGST VKLGTARAVE IKELHLAYES WFPQFMDGET LIAAE

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE007869 Genomic DNA. Translation: AAK87617.2. Different initiation.
PIRAH2803.
H97582.
RefSeqNP_354832.2. NC_003062.2.

3D structure databases

ProteinModelPortalQ8UEB0.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8UEB0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1133888.
GenomeReviewsGene locus Atu1850 in contig AE007869_GR.
KEGGatu:Atu1850.
PATRIC20813477. VBIAgrTum91616_1863.

Phylogenomic databases

eggNOGCOG0046.
HOGENOMHBG311214.
OMAYGNSFGV.
PhylomeDBQ8UEB0.
ProtClustDBPRK01213.

Enzyme and pathway databases

BioCycATUM176299-1:ATU1850-MONOMER.

Family and domain databases

HAMAPMF_00420. PurL_2.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view]
KOK01952.
PfamPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01736. FGAM_synth_II. 1 hit.
ProtoNetSearch...

Entry information

Entry namePURL_AGRT5
AccessionPrimary (citable) accession number: Q8UEB0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2002
Last modified: January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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