Q8UEA1 (GLSA_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutaminase
EC=3.5.1.2

Gene names

Name:	glsA
Ordered Locus Names:	Atu1859
ORF Names:	AGR_C_3412

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium › Agrobacterium tumefaciens complex › Agrobacterium fabrum

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glutaminase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	glutaminase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Glutaminase HAMAP-Rule MF_00313		PRO_0000110586
Sites
	Binding site	64	1	Substrate By similarity
	Binding site	114	1	Substrate By similarity
	Binding site	160	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	191	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	261	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UEA1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 65229FEFE6CC1135	FASTA	309	32,926
	10 20 30 40 50 60 MQDIQGIVDS IYDSMLPRLG EGKVADYIPE LAKVDPNQFG IAITTVDGTT YTAGNALVPF 70 80 90 100 110 120 SIQSISKVFM LTLALGKAGE TVWNRVGREP SGSSFNSIVQ LEHEHGIPRN PFVNAGAIVV 130 140 150 160 170 180 TDIVLSGHQP REAIGELLRF VRYLADDDTI SIDDTVAKSE QATGFRNFAL ANFMRSFGNL 190 200 210 220 230 240 HHPVEHTLGV YFHQCALSMT CAQLSRAGLF LANRGRNPLS GHTVVSDRRA RRINALMLTC 250 260 270 280 290 300 GHYDGSGDFA YHVGLPGKSG VGGGIMAVAP GNASIAVWSP GLNKVGNSAL GSQALELLAT KTGWSVFGA

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK87626.1.
PIR	A97584. AI2804.
RefSeq	NP_354841.1. NC_003062.2.
3D structure databases
ProteinModelPortal	Q8UEA1.
ModBase	Search...
Protein-protein interaction databases
STRING	176299.Atu1859.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1133897.
KEGG	atu:Atu1859.
PATRIC	20813495. VBIAgrTum91616_1872.
Phylogenomic databases
eggNOG	COG2066.
HOGENOM	HOG000216890.
KO	K01425.
OMA	EPSGNPF.
ProtClustDB	PRK00971.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
HAMAP	MF_00313. Glutaminase.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR015868. Glutaminase. [Graphical view]
PANTHER	PTHR12544. PTHR12544. 1 hit.
Pfam	PF04960. Glutaminase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
TIGRFAMs	TIGR03814. Gln_ase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLSA_AGRT5

Accession

Primary (citable) accession number: Q8UEA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program