Reviewed, UniProtKB/Swiss-Prot Q8UDM9 (MURC_AGRT5)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	Atu2093
ORF Names:	AGR_C_3796

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable HAMAP MF_00046.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182044
Regions
	Nucleotide binding	114 – 120	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8UDM9-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 3FB7F7FBEE7C3DA6	FASTA	471	50,800
	10 20 30 40 50 60 MKMPKAIGLV HFIGIGGIGM SGIAEVLHNL GHRVQGSDQA DSANVQRLRD KGIEVFVGHT 70 80 90 100 110 120 ADNLGDAEVV VVSTAIKKNN PELIAAREKH LPIVRRAEML AELMRFRNAI AIGGTHGKTT 130 140 150 160 170 180 TTSMVATLLE AGNLDPTVIN GGIINAYGTN ARMGEGEWMV VEADESDGTF LKLPADVAVI 190 200 210 220 230 240 TNIDPEHLDH YGNFDAVRAA FRQFVENVPF YGFGVMCLDH PEVQALVGRI EDRKVITYGE 250 260 270 280 290 300 NPQADVRFSN VRIDGTRSIF DVEIRRRRTG KIFSFKDLVL PMPGRHNVSN ATAAIAVANR 310 320 330 340 350 360 LGISEADIKK GLASFAGVKR RFTLTGEANG VQVFDDYGHH PVEIKAVLAA AREACKGRII 370 380 390 400 410 420 AVHQPHRYSR LSSLFDDFAH CFNDADTILL APVYAAGEDP IEGASSEALV SAIKAAGHRD 430 440 450 460 470 ARFLEKREDL ASQVAAIANP GDFVVLLGAG NITQWAAALP SELKSISGKS E

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK87843.1.
PIR	AF2833. B97611.
RefSeq	NP_355058.1.
3D structure databases
SMR	Q8UDM9. Positions 6-462.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UDM9.
Genome annotation databases
GeneID	1134131.
GenomeReviews	Gene locus Atu2093 in contig AE007869_GR.
KEGG	atc:AGR_C_3796. atu:Atu2093.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0773.
HOGENOM	HBG476594.
OMA	YQPHRYS.
PhylomeDB	Q8UDM9.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU2093-MONOMER.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_AGRT5

Accession

Primary (citable) accession number: Q8UDM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	June 1, 2002
Last modified:	February 9, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)