Reviewed, UniProtKB/Swiss-Prot Q8UDM3 (MURE_AGRT5)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	Atu2099
ORF Names:	AGR_C_3809

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP MF_00208
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity HAMAP MF_00208.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP MF_00208
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 489	489	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_0000101860
Regions
	Nucleotide binding	110 – 116	7	ATP Potential
	Region	152 – 153	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	407 – 410	4	Meso-diaminopimelate binding By similarity
	Motif	407 – 410	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	34	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	179	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	185	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	187	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	383	1	Meso-diaminopimelate By similarity
	Binding site	455	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	459	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	219	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UDM3-1 [UniParc]. Last modified October 19, 2002. Version 2. Checksum: 1CA8820B5FD0EBEF	FASTA	489	51,287
	10 20 30 40 50 60 MNLRDISGNA FPELKELLLS EIGAIEIGGI TADSRKAAPG SLFVAVAGTK ADGAAYVKDA 70 80 90 100 110 120 VAKGAVAVVS GHAVEADVPV LVVTDPRLYL SLAASRFYGK QPDTMVAVTG TAGKTSVASF 130 140 150 160 170 180 VRQIWAFAGH AAAQIGTTGV IAPGREDYGA LTTPDPVTLH ALLAELASEG VTHAAMEASS 190 200 210 220 230 240 HGLDQRRLDG VHLSAAGFTN LGRDHMDYHP TIEDYMAAKM RLFDTLMEKG APAVIFADDP 250 260 270 280 290 300 WSDKAIGAAR EAGLEVRTVG RNGQYLTLKR VEHFRHKQMI EVHHDGVIFE VDIPLAGDFQ 310 320 330 340 350 360 VANALVAAGL AMSTGVPAAT ALKALEKLVG AAGRLELVGQ TKNGALAYVD YAHKPDALEN 370 380 390 400 410 420 VLTSVRPFTS GRIITVFGCG GDRDKGKRPI MGEVATRLSD IVIVTDDNPR SEDAATIRSE 430 440 450 460 470 480 VMAAAAGALE IGDRAEAIRH AVSMLSHGDT LIVAGKGHEE GQIVGSVTLP FSDHEQVRSA LAELEGSKI

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK87849.2.
PIR	AD2834. H97611.
RefSeq	NP_355064.2.
3D structure databases
SMR	Q8UDM3. Positions 23-487.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UDM3.
Genome annotation databases
GeneID	1134137.
GenomeReviews	Gene locus Atu2099 in contig AE007869_GR.
KEGG	atc:AGR_C_3809. atu:Atu2099.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0769.
HOGENOM	HBG602753.
OMA	HTPDGIE.
PhylomeDB	Q8UDM3.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU2099-MONOMER.
Family and domain databases
HAMAP	MF_00208. MurE. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_AGRT5

Accession

Primary (citable) accession number: Q8UDM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	October 19, 2002
Last modified:	February 9, 2010
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)