Urease subunit alpha - Agrobacterium tumefaciens (strain C58 / ATCC 33970)

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Reviewed, UniProtKB/Swiss-Prot Q8UCT2 (URE1_AGRT5)

Last modified November 3, 2009. Version 52. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Urease subunit alpha
    EC=3.5.1.5
Alternative name(s):
    Urea amidohydrolase subunit alpha

Gene names

Name:	ureC
Ordered Locus Names:	Atu2401
ORF Names:	AGR_C_4357

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

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Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Catalytic activity	Urea + H₂O = CO₂ + 2 NH₃. HAMAP MF_01953
Cofactor	Binds 2 nickel ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953
Subunit structure	Heterotrimer of ureA (gamma), ureB (beta) and ureC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamylation allows a single lysine to coordinate two nickel ions By similarity.
Sequence similarities	Belongs to the urease family. Contains 1 urease domain.

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Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nickel
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	urea metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW urease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 569

569

Urease subunit alpha HAMAP MF_01953

PRO_0000234130

Regions

Domain

131 – 569

439

Urease

Sites

Active site

321

Proton donor By similarity

Metal binding

136

Nickel 2 By similarity

Metal binding

138

Nickel 2 By similarity

Metal binding

218

Nickel 1; via carbamate group By similarity

Metal binding

218

Nickel 2; via carbamate group By similarity

Metal binding

247

Nickel 1 By similarity

Metal binding

273

Nickel 1 By similarity

Metal binding

361

Nickel 2 By similarity

Binding site

220

Substrate By similarity

Amino acid modifications

Modified residue

218

N6-carboxylysine By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q8UCT2-1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 91476D99C81D26DB
Show »

FASTA

569

60,815

        10         20         30         40         50         60 
MPYKISRAAY AGMFGPTVGD KVRLADTELF IEIEKDHTTY GEEVKFGGGK VIRDGMGQSQ 

        70         80         90        100        110        120 
ATRAEGAVDT VITNAVIVDH SGIYKADVGL KNGRIHAIGK AGNPDTQPGV TIIVGPSTEA 

       130        140        150        160        170        180 
IAGEGKILTA GGMDAHIHYI CPQQIEEALM SGVTCMLGGG SGPAHGTLAT TCTGAWHIER 

       190        200        210        220        230        240 
MIESFDAFPM NLALAGKGNA SLPAPLEEMI LAGASSLKLH EDWGTTPAAI DNCLTVADEY 

       250        260        270        280        290        300 
DVQVMIHTDT LNESGFVEDT VAAIRGRTIH AFHTEGAGGG HAPDIIKVCG NPNVIPSSTN 

       310        320        330        340        350        360 
PTRPYTVNTL AEHLDMLMVC HHLSPSIPED IAFAESRIRK ETIAAEDILH DIGAFSIISS 

       370        380        390        400        410        420 
DSQAMGRVGE VAIRTWQTAD KMKRQRGRLK EETGENDNFR VRRYIAKYTI NPAIAQGVSH 

       430        440        450        460        470        480 
EIGSVEVGKR ADLVLWNPAF FGVKPEMVLL GGSIAAAPMG DPNASIPTPQ PMHYRPMFAA 

       490        500        510        520        530        540 
YGKLRTNSSV TFVSQASLDG GLAQRLGVAK KLLAVKNVRG GISKASMIHN SLTPHIEVDP 

       550        560 
ETYEVRADGE LLTCEPATVL PMAQRYFLF

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References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58."
Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J.

Nester E.W.
Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58."
Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C.

Slater S.
Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases
	AE007869 Genomic DNA. Translation: AAK88138.1.
PIR	A97648. AG2871.
RefSeq	NP_355353.1.
3D structure databases
HSSP	HSSP built from PDB template 1EJX based on UniProtKB P18314.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UCT2.
Protein family/group databases
MEROPS	M38.982.
Genome annotation databases
GeneID	1134439.
GenomeReviews	Gene locus Atu2401 in contig AE007869_GR.
KEGG	atc:AGR_C_4357. atu:Atu2401.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8UCT2.
OMA	SHIHFIC.
Family and domain databases
HAMAP	MF_01953. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR011612. Urease_alpha_N. IPR005848. Urease_asu. IPR017951. Urease_asu_c. IPR017952. Urease_asu_core. IPR017950. Urease_asu_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. PF00449. Urease_alpha. 1 hit. [Graphical view]
PRINTS	PR01752. UREASE.
TIGRFAMs	TIGR01792. urease_alph. 1 hit.
PROSITE	PS01120. UREASE_1. 1 hit. PS00145. UREASE_2. 1 hit. PS51368. UREASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

URE1_AGRT5

Accession

Primary (citable) accession number: Q8UCT2
Secondary accession number(s): Q7CX53

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents