Reviewed, UniProtKB/Swiss-Prot Q8UCE7 (PUR72_AGRT5)

Names and origin

Protein names

Recommended name:
    Putative phosphoribosylaminoimidazole-succinocarboxamide synthase 2
    EC=6.3.2.6
Alternative name(s):
    SAICAR synthetase 2

Gene names

Name:	purC2
Ordered Locus Names:	Atu2548
ORF Names:	AGR_C_4615

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. HAMAP MF_00137
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2. HAMAP MF_00137
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Putative phosphoribosylaminoimidazole-succinocarboxamide synthase 2 HAMAP MF_00137		PRO_0000100795

Sequences

	Sequence		Length	Mass (Da)
	Q8UCE7-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: ACF1A66E94889921	FASTA	316	35,975
	10 20 30 40 50 60 MRILDEAVIP ELPNYYRGKV RENYDLPDGN RIIISTDRLS AFDQILTCIP YKGQVLTQTA 70 80 90 100 110 120 RYWFEQTKDI CPNHVVSYPD PNVVIGKRLD ILPVEVVVRG YLAGTTGTSI LTLYKKGERQ 130 140 150 160 170 180 MYGMSLPDGM KDNQILPEPV ITPTSKAFDG GHDEPLTPSE IVEKKLLTQE QWDTLSRYAL 190 200 210 220 230 240 ALFRHGQEIA AKQGLILVDT KYEFGTDENG TIILADEIHT PDSSRYWMAD SYDESFREGK 250 260 270 280 290 300 RPASFDKDFV RAWVGERCDP YKDAVPKIPE DLVLQTSQVY IDAYERITGQ RFVPDDSGET 310 PLARVRRNLE PFFPGV

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK88273.1.
PIR	AC2889. H97664.
RefSeq	NP_355488.1.
3D structure databases
SMR	Q8UCE7. Positions 4-293.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UCE7.
Genome annotation databases
GeneID	1134586.
GenomeReviews	Gene locus Atu2548 in contig AE007869_GR.
KEGG	atc:AGR_C_4615. atu:Atu2548.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0152.
HOGENOM	HBG555040.
OMA	LTLMDEV.
PhylomeDB	Q8UCE7.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU2548-MONOMER.
Family and domain databases
HAMAP	MF_00137. SAICAR_synth. [Tree]
InterPro	IPR013816. ATP_grasp_subdomain_2. IPR001636. SAICAR_synt. IPR018236. SAICAR_synthetase_CS. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
PANTHER	PTHR11609. SAICAR_synt. 1 hit.
Pfam	PF01259. SAICAR_synt. 1 hit. [Graphical view]
PROSITE	PS01057. SAICAR_SYNTHETASE_1. 1 hit. PS01058. SAICAR_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR72_AGRT5

Accession

Primary (citable) accession number: Q8UCE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2002
Last modified:	January 19, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)