Reviewed, UniProtKB/Swiss-Prot Q8UC47 (GCP_AGRT5)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	Atu2651
ORF Names:	AGR_C_4806

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc Probable.
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_0000303249
Sites
	Metal binding	119	1	Zinc Potential
	Metal binding	123	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8UC47-1 [UniParc]. Last modified July 5, 2004. Version 2. Checksum: 4E8F214AD5DAD9F1	FASTA	365	38,433
	10 20 30 40 50 60 MPPFLRILGI ETSCDETAAS IVVRHADGRG EIVSDVVLSQ LEEHSAYGGV VPEIAARAHV 70 80 90 100 110 120 EALDTLVEEA LDQAGVKLAD VDAIAATSGP GLIGGLLVGL MTGKAIAKAA GKPLYAINHL 130 140 150 160 170 180 EGHALTARLT DGLSFPYLML LVSGGHTQLV LVRGVGEYER WGTTIDDALG EAFDKTAKLL 190 200 210 220 230 240 GLPYPGGPAV ENAAAKGDPD RFPLPRPMVG EARLDFSFSG LKTAVRQAAT AIAPLSEQDI 250 260 270 280 290 300 ADICASFQKA VSRTLKDRIG RGLARFKVEF PHINGEPALV VAGGVAANQE IRQTLQALCD 310 320 330 340 350 360 THGFRFVAPP HRLCTDNAAM IAWAGLERMA EGRQADALEV APRSRWPLDG SAETLIGFGK RGAKA

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007869 Genomic DNA. Translation: AAK88373.2.
PIR	AB2902. D97677.
RefSeq	NP_355588.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UC47.
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	1134689.
GenomeReviews	Gene locus Atu2651 in contig AE007869_GR.
KEGG	atc:AGR_C_4806. atu:Atu2651.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8UC47.
OMA	EHSAYGG.
Family and domain databases
HAMAP	MF_01445. [Tree]
InterPro	IPR009180. Pept_M22_Osialgl. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
ProDom	PD002367. Peptidase_M22. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_AGRT5

Accession

Primary (citable) accession number: Q8UC47
Secondary accession number(s): Q7CWJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)