Q8UBV5 (ACSA_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-coenzyme A synthetase
EC=6.2.1.1

Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme

Gene names

Name:	acsA
Synonyms:	acs
Ordered Locus Names:	Atu2745
ORF Names:	AGR_C_4980

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970)

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	651 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 651	651	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_0000208352
Sites
	Active site	517	1	By similarity
Amino acid modifications
	Modified residue	609	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UBV5 [UniParc]. Last modified September 26, 2003. Version 2. Checksum: D97CD83D4B38D4EF	FASTA	651	72,844
	10 20 30 40 50 60 MSAKIYPVLK SAKARTLIDN ERYQKWYQES VEDPEKFWDK HGRRIDWFKP YTKVKNTSFK 70 80 90 100 110 120 GRVPIKWFED GLTNVSYNCI DRHLKTHGER TAIIWEGDNP YIDKKITYNQ LYDYVCRLAN 130 140 150 160 170 180 VLKKHGVKKG DRVTIYMPMI PEAAYAMLAC ARIGAVHSVV FGGFSPEALA GRIVDCESTF 190 200 210 220 230 240 VITCDEGVRG GKPIPLKENT DKAIDIAAKQ YVIVNKVLVV RRTGGKTGWA PGRDIWYHQE 250 260 270 280 290 300 IATVKPDCPP VKMRAEDPLF ILYTSGSTGK PKGVLHTTGG YLVYTSMTHE YVFDYKDGEV 310 320 330 340 350 360 FWCTADVGWV TGHSYIVYGP LANCATTLMF EGVPNFPDQG RFWEVIDKHK VNIFYTAPTA 370 380 390 400 410 420 LRSLMGAGDQ FVQRSSRESL RLLGTVGEPI NPEAWEWYYH VVGEDKSPIV DTWWQTETGG 430 440 450 460 470 480 ILISPLPGAT DLKPGSATRP FFGVQPQLVD AEGNVLEGPA DGNLCIIDSW PGQSRSVYGD 490 500 510 520 530 540 HQRFVDTYFS TYKGKYFTGD GCRRDEDGYY WITGRVDDVL NVSGHRLGTA EVESALVSHH 550 560 570 580 590 600 QVSEAAVVGY PHPIKGQGIY CYVTLMAGQS GDYALREELV KHVRNEIGPV ATPDKIQFAP 610 620 630 640 650 GLPKTRSGKI MRRILRKIAE DDFGALGDTS TLADPAVVED LIANRQNRTA S

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK88460.2.
PIR	AH2913. C97688.
RefSeq	NP_355675.2. NC_003062.2.
3D structure databases
ProteinModelPortal	Q8UBV5.
SMR	Q8UBV5. Positions 15-646.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UBV5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1134783.
GenomeReviews	Gene locus Atu2745 in contig AE007869_GR.
KEGG	atu:Atu2745.
PATRIC	20815285. VBIAgrTum91616_2755.
Phylogenomic databases
HOGENOM	HBG547964.
OMA	KGKPYML.
PhylomeDB	Q8UBV5.
ProtClustDB	PRK00174.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU2745-MONOMER.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_AGRT5

Accession

Primary (citable) accession number: Q8UBV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	September 26, 2003
Last modified:	January 25, 2012
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program