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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei635 – 6351ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAGRO:ATU2748-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Atu2748
ORF Names:AGR_C_4985
OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000000813: Chromosome circular

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 876876Leucine--tRNA ligasePRO_0000151960Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi176299.Atu2748.

Structurei

3D structure databases

ProteinModelPortaliQ8UBV2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi632 – 6365"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8UBV2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAIERYNPRD AEPRWQQKWN EDKVFVTDNS DPREKYYVLE MFPYPSGRIH
60 70 80 90 100
MGHVRNYAMG DVVARYKRAR GFNVLHPMGW DAFGMPAENA AMQNKVHPKD
110 120 130 140 150
WTYQNIATMR GQLKSMGLSL DWTREFATCD VEYYHRQQAL FVDFMEKGLV
160 170 180 190 200
YRKQSKVNWD PVDHTVLANE QVIDGRGWRS GALVEQRELT QWFFRITDFS
210 220 230 240 250
QDLLDELDEL DQWPEKVRLM QKNWIGRSEG LSLRWQTVAD TAPQGFSDIT
260 270 280 290 300
VYTTRPDTLF GASFLAIAAD HPLAKELSAT NPAIAEFCDE CRRHGTSLAA
310 320 330 340 350
LETAEKKGID TGVKVVHPLD PSWELPVYVA NFVLMDYGTG AIFGCPSGDQ
360 370 380 390 400
RDLDFARKYG LPVVAVVAPE GPDAASFTVE DTAFTDDGVM INSSFLNGMK
410 420 430 440 450
TTDAFEAVVQ KLSAQSLGNA PQAERKVNFR LRDWGISRQR YWGCPIPVIH
460 470 480 490 500
CEVCGVVPVP KKDLPVKLPD DVTFDVPGNP LDRHSTWRHV SCPQCGHDAR
510 520 530 540 550
RETDTMDTFV DSSWYYTRFT APWEDEPTDP QVANHWLPVD QYIGGIEHAI
560 570 580 590 600
LHLLYSRFFT RAMRETGHVG VKEPFKGLFT QGMVVHETYS RGEGLTREWV
610 620 630 640 650
PPAELRIEEN DGTRRAFLLS SGEEVKIGSI EKMSKSKKNV VDPDDIIASY
660 670 680 690 700
GADTARFFVL SDSPPDRDVI WSEAGVEGAN RFVQRVWRII GEAAEQLKGV
710 720 730 740 750
KPKPATEGEG LAASKAAHKT LKAVQEDLDK LAFNKAIARI YELVNALAGP
760 770 780 790 800
LADVAAGGKP DNVKAAARDA VEILIRIIAP MTPHLAEECW SALGNEGLVA
810 820 830 840 850
ETPWPTFVAS LVEENDVVMP VQVNGKKRGE LTIARDADQD AVRTAALELD
860 870
AVKSILAGGE PKKVIVVPQR IVNIVV
Length:876
Mass (Da):98,018
Last modified:June 1, 2002 - v1
Checksum:iAB4856E88F7EE9DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK88463.1.
PIRiAC2914.
F97688.
RefSeqiNP_355678.1. NC_003062.2.

Genome annotation databases

EnsemblBacteriaiAAK88463; AAK88463; Atu2748.
GeneIDi1134786.
KEGGiatu:Atu2748.
PATRICi20815291. VBIAgrTum91616_2758.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK88463.1.
PIRiAC2914.
F97688.
RefSeqiNP_355678.1. NC_003062.2.

3D structure databases

ProteinModelPortaliQ8UBV2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176299.Atu2748.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK88463; AAK88463; Atu2748.
GeneIDi1134786.
KEGGiatu:Atu2748.
PATRICi20815291. VBIAgrTum91616_2758.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciAGRO:ATU2748-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.

Entry informationi

Entry nameiSYL_AGRT5
AccessioniPrimary (citable) accession number: Q8UBV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2002
Last modified: January 7, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.