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Q8UBV2 (SYL_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Atu2748
ORF Names:AGR_C_4985
OrganismAgrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]
Taxonomic identifier176299 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

Protein attributes

Sequence length876 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 876876Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000151960

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif632 – 6365"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6351ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8UBV2 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: AB4856E88F7EE9DD

FASTA87698,018
        10         20         30         40         50         60 
MAIERYNPRD AEPRWQQKWN EDKVFVTDNS DPREKYYVLE MFPYPSGRIH MGHVRNYAMG 

        70         80         90        100        110        120 
DVVARYKRAR GFNVLHPMGW DAFGMPAENA AMQNKVHPKD WTYQNIATMR GQLKSMGLSL 

       130        140        150        160        170        180 
DWTREFATCD VEYYHRQQAL FVDFMEKGLV YRKQSKVNWD PVDHTVLANE QVIDGRGWRS 

       190        200        210        220        230        240 
GALVEQRELT QWFFRITDFS QDLLDELDEL DQWPEKVRLM QKNWIGRSEG LSLRWQTVAD 

       250        260        270        280        290        300 
TAPQGFSDIT VYTTRPDTLF GASFLAIAAD HPLAKELSAT NPAIAEFCDE CRRHGTSLAA 

       310        320        330        340        350        360 
LETAEKKGID TGVKVVHPLD PSWELPVYVA NFVLMDYGTG AIFGCPSGDQ RDLDFARKYG 

       370        380        390        400        410        420 
LPVVAVVAPE GPDAASFTVE DTAFTDDGVM INSSFLNGMK TTDAFEAVVQ KLSAQSLGNA 

       430        440        450        460        470        480 
PQAERKVNFR LRDWGISRQR YWGCPIPVIH CEVCGVVPVP KKDLPVKLPD DVTFDVPGNP 

       490        500        510        520        530        540 
LDRHSTWRHV SCPQCGHDAR RETDTMDTFV DSSWYYTRFT APWEDEPTDP QVANHWLPVD 

       550        560        570        580        590        600 
QYIGGIEHAI LHLLYSRFFT RAMRETGHVG VKEPFKGLFT QGMVVHETYS RGEGLTREWV 

       610        620        630        640        650        660 
PPAELRIEEN DGTRRAFLLS SGEEVKIGSI EKMSKSKKNV VDPDDIIASY GADTARFFVL 

       670        680        690        700        710        720 
SDSPPDRDVI WSEAGVEGAN RFVQRVWRII GEAAEQLKGV KPKPATEGEG LAASKAAHKT 

       730        740        750        760        770        780 
LKAVQEDLDK LAFNKAIARI YELVNALAGP LADVAAGGKP DNVKAAARDA VEILIRIIAP 

       790        800        810        820        830        840 
MTPHLAEECW SALGNEGLVA ETPWPTFVAS LVEENDVVMP VQVNGKKRGE LTIARDADQD 

       850        860        870 
AVRTAALELD AVKSILAGGE PKKVIVVPQR IVNIVV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE007869 Genomic DNA. Translation: AAK88463.1.
PIRAC2914.
F97688.
RefSeqNP_355678.1. NC_003062.2.

3D structure databases

ProteinModelPortalQ8UBV2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176299.Atu2748.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK88463; AAK88463; Atu2748.
GeneID1134786.
KEGGatu:Atu2748.
PATRIC20815291. VBIAgrTum91616_2758.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHELEVYT.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycAGRO:ATU2748-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_AGRT5
AccessionPrimary (citable) accession number: Q8UBV2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries