Reviewed, UniProtKB/Swiss-Prot Q8UBS0 (PROB_AGRT5)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase
      Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	Atu2780
ORF Names:	AGR_C_5045

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Glutamate 5-kinase HAMAP MF_00456		PRO_0000109628
Regions
	Domain	282 – 359	78	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q8UBS0-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 863E47328DB71333	FASTA	389	40,955
	10 20 30 40 50 60 MSLTRKPLAS HHRIVIKIGS ALLVDRKSGL KKDWLDAICE DIAALKKNGA DVQVVSSGAI 70 80 90 100 110 120 ALGRTVLGLP SGALKLEESQ AAAAVGQIAL ARAWSESLSR HEIVAGQILL TLSDTEERRR 130 140 150 160 170 180 YLNARATINQ LLKIGAIPII NENDTVATTE IRYGDNDRLA ARVATMTGAD LLVLLSDIDG 190 200 210 220 230 240 LYTAPPHLDP DAKFLETIAD ITPEIEAMAG GAASELSRGG MRTKIDAGKI ATAAGCGMII 250 260 270 280 290 300 ASGKTLNPLK AIENGARSSW FAPSGTPVTA RKTWIAGQLQ PAGEIHVDAG AEKALYAGKS 310 320 330 340 350 360 LLPAGVRQVK GNFGRGDAIA IIGVEGREVA RGLAGYDAEE ARLIIGHKSN EIETILGYVG 370 380 RAAMIHRDDL VMTGAAVKVK HAKKDEVHA

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK88495.1.
PIR	AC2918. F97692.
RefSeq	NP_355710.1.
3D structure databases
SMR	Q8UBS0. Positions 11-372.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UBS0.
Genome annotation databases
GeneID	1134818.
GenomeReviews	Gene locus Atu2780 in contig AE007869_GR.
KEGG	atc:AGR_C_5045. atu:Atu2780.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HBG507643.
OMA	TDVDRLY.
PhylomeDB	Q8UBS0.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU2780-MONOMER.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. IPR015947. PUA-like. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_AGRT5

Accession

Primary (citable) accession number: Q8UBS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	June 1, 2002
Last modified:	February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)