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Protein

Ribosomal RNA small subunit methyltransferase G

Gene

rsmG

Organism
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the N7 position of guanine in position 527 of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei66 – 661S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei71 – 711S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei135 – 1351S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAGRO:ATU2830-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase GUniRule annotation (EC:2.1.1.170UniRule annotation)
Alternative name(s):
16S rRNA 7-methylguanosine methyltransferaseUniRule annotation
Short name:
16S rRNA m7G methyltransferaseUniRule annotation
Gene namesi
Name:rsmGUniRule annotation
Ordered Locus Names:Atu2830
ORF Names:AGR_C_5132
OrganismiAgrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
Proteomesi
  • UP000000813 Componenti: Chromosome circular

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205Ribosomal RNA small subunit methyltransferase GPRO_0000184205Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi176299.Atu2830.

Structurei

3D structure databases

ProteinModelPortaliQ8UBM1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni119 – 1202S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107YTN. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221015.
KOiK03501.
OrthoDBiEOG6HF639.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8UBM1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKINGQSVSR ETQERLEHFV ELFKKWNKTT NLVAPSTLTE LWSRHVADSA
60 70 80 90 100
QIFQLSPQPK KWIDLGSGGG FPGVITAIFL AELGDGWVDL VESNNKKAAF
110 120 130 140 150
LRIALKETGA RGAVHPVRIE TASEIITTCN AVSARALADL DLLFGYIHPW
160 170 180 190 200
AQNNPNLKAY FNKGRDYDSE VQKAHGRWQF DLIKHRSAIE ADSVVLEISN

LVLRR
Length:205
Mass (Da):22,965
Last modified:June 1, 2002 - v1
Checksum:iEA6B927D2C8B7B45
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK88541.1.
PIRiAE2924.
D97698.
RefSeqiNP_355756.1. NC_003062.2.
WP_010972597.1. NC_003062.2.

Genome annotation databases

EnsemblBacteriaiAAK88541; AAK88541; Atu2830.
GeneIDi1134868.
KEGGiatu:Atu2830.
PATRICi20815453. VBIAgrTum91616_2839.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK88541.1.
PIRiAE2924.
D97698.
RefSeqiNP_355756.1. NC_003062.2.
WP_010972597.1. NC_003062.2.

3D structure databases

ProteinModelPortaliQ8UBM1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176299.Atu2830.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK88541; AAK88541; Atu2830.
GeneIDi1134868.
KEGGiatu:Atu2830.
PATRICi20815453. VBIAgrTum91616_2839.

Phylogenomic databases

eggNOGiENOG4107YTN. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221015.
KOiK03501.
OrthoDBiEOG6HF639.

Enzyme and pathway databases

BioCyciAGRO:ATU2830-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.

Entry informationi

Entry nameiRSMG_AGRFC
AccessioniPrimary (citable) accession number: Q8UBM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2002
Last modified: November 11, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.