Reviewed, UniProtKB/Swiss-Prot Q8UAY9 (DNE21_AGRT5)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase 1
    EC=2.7.7.7

Gene names

Name:	dnaE2-1
Ordered Locus Names:	Atu3228
ORF Names:	AGR_L_3173

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	1091 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1091	1091	Error-prone DNA polymerase 1 HAMAP MF_01902		PRO_0000103362

Sequences

	Sequence		Length	Mass (Da)
	Q8UAY9-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 7E1C7FD9EDB33CB2	FASTA	1,091	122,865
	10 20 30 40 50 60 MSAPRYAELQ VTTHFSFLRG ASSCDELFEQ AKNLGIEALG VVDRNSLAAI PRAYEAANNH 70 80 90 100 110 120 GVRLVIGCRL DLDDDLSVLV YPMDRAAYGR LCRLLSVGKK RGGKGKCRLS WDDLVAYGEG 130 140 150 160 170 180 LIVVLLADLA DDLCALRLRR LKAAFADRAY MALSLRRRPN DQMRLFELSG MAQAAGVPTV 190 200 210 220 230 240 VTNDVLFHVP ERRMLQDVVT CIRHNCTIDE AGFRRERHAD RYMKPPEEMH RLFARYPEAL 250 260 270 280 290 300 SRSLEIAKRC KFSLKELVYQ YPEERSLPGL TAQQALEKMV WEAVPGRYPN GLPEKVEKAL 310 320 330 340 350 360 HHELDVVGRL QYASYFLTVN AIVRYARSKD ILCQGRGSAA NSVICFVLGI TAIDPALFSN 370 380 390 400 410 420 LVFERFVSEN RGEPPDIDVD FEHQRREEVI QWVYDTYGRD KAALCSVVTR YRGRGALRDV 430 440 450 460 470 480 GKVLGLPEDL TKLLSSQVWR WSEGVGEKQV KELNLNMEDR RLKLAFELAN QLVGTPRHHS 490 500 510 520 530 540 QHPGGFVLSH DRLDELVPIE PAAMNDRQII EWDKDDIDIV KFMKMDCLAL GMLSCMKRGF 550 560 570 580 590 600 DLLEARTGEK YDLAAMPPDD PATFAMIQKA DTLGTFQIES RAQMSMLPRL KPAKFYDLVI 610 620 630 640 650 660 QVAIVRPGPI QGDMVHPYLR RRQGKEPVLY EKPQLENILK KTLGVPLFQE QAMRIAMDCA 670 680 690 700 710 720 DFTADEADQL RRAMATFKNV GTISKFKEKL VTGMVANGYD KEFAERIFKQ LEGFGSYGFP 730 740 750 760 770 780 ESHAASFALI AYASSWLKCH HPDIFCTAIL NSQPMGFYAP AQIVRDARDH GVEVRPVCVN 790 800 810 820 830 840 NSRFDCTLEP TGKKNDKGEE RFAVRLGLRM VKGLSNDHAA DIVAARQDRP FASVDDLWRR 850 860 870 880 890 900 AGVPAAALVC LAEADAFLPS LRLARREALW AIKALRDEPL PLFAAAAIRE NAVIEELQEP 910 920 930 940 950 960 SVALRPMTDG GEVVQDYGHV GLTLREHPMT FLRRDLSRRR IVTCAEAVRV RDGTWLETAG 970 980 990 1000 1010 1020 LVLVRQRPGS AKGVIFMTLE DETGIANAVL WVKTFEKYRR VVLSAGMVGI YGKIQREGEV 1030 1040 1050 1060 1070 1080 VHLVAHRLTD LSHALASVGE RNNAFPLPHG RGDEFHHGMP DDHRAIRKRP PPSNHDDDEV 1090 ERIKVISRNF H

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007870 Genomic DNA. Translation: AAK90161.1.
PIR	AF2953. G98329.
RefSeq	NP_357376.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UAY9.
Genome annotation databases
GeneID	1135102.
GenomeReviews	Gene locus Atu3228 in contig AE007870_GR.
KEGG	atc:AGR_L_3173. atu:Atu3228.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8UAY9.
OMA	VIHYRGK.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNE21_AGRT5

Accession

Primary (citable) accession number: Q8UAY9
Secondary accession number(s): Q7CRY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)