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Protein

Probable hydroxyethylthiazole kinase

Gene

thiM

Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).By similarity

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.

Cofactori

Mg2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501Substrate; via amide nitrogenBy similarity
Binding sitei125 – 1251ATPBy similarity
Binding sitei145 – 1451ATPBy similarity
Binding sitei172 – 1721Substrate; via amide nitrogenBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydroxyethylthiazole kinase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAGRO:ATU3288-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable hydroxyethylthiazole kinase (EC:2.7.1.50)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name:
TH kinase
Short name:
Thz kinase
Gene namesi
Name:thiM
Ordered Locus Names:Atu3288
ORF Names:AGR_L_3060
OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000000813: Chromosome linear

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 246246Probable hydroxyethylthiazole kinasePRO_0000156923Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi176299.Atu3288.

Structurei

3D structure databases

ProteinModelPortaliQ8UAS9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.Curated

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiSPVMAHA.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 2 hits.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 2 hits.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8UAS9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGDFPTAAR AAEILERVRQ MRPRVHCLMN TVVQKFTADG ITVIGGIPSM
60 70 80 90 100
TTSLEEIESF VTKADALTVN LGTLDAERRR VIRLAIEIAN ASGKPWIVDP
110 120 130 140 150
VHVDYSPSRL DFARELIAQS PTIVRGNRAE MSLIGDVPDV VRIETGPVDH
160 170 180 190 200
LRDRTRNVSI VNGHPWMAKV TGTGCLSGGV IAAFMAVEKD ALTAAASALA
210 220 230 240
VTGVSAELAA QRAKGPGTFE PAFLDALSEI SGEDIINHAR IEHEQG
Length:246
Mass (Da):26,345
Last modified:June 1, 2002 - v1
Checksum:i27E5AC411493BCAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007870 Genomic DNA. Translation: AAK90102.1.
PIRiAB2961.
D98322.
RefSeqiNP_357317.1. NC_003063.2.

Genome annotation databases

EnsemblBacteriaiAAK90102; AAK90102; Atu3288.
GeneIDi1135162.
KEGGiatu:Atu3288.
PATRICi20816034. VBIAgrTum91616_3129.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007870 Genomic DNA. Translation: AAK90102.1.
PIRiAB2961.
D98322.
RefSeqiNP_357317.1. NC_003063.2.

3D structure databases

ProteinModelPortaliQ8UAS9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176299.Atu3288.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK90102; AAK90102; Atu3288.
GeneIDi1135162.
KEGGiatu:Atu3288.
PATRICi20816034. VBIAgrTum91616_3129.

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiSPVMAHA.
OrthoDBiEOG628F8M.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
BioCyciAGRO:ATU3288-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 2 hits.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 2 hits.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.

Entry informationi

Entry nameiTHIM_AGRT5
AccessioniPrimary (citable) accession number: Q8UAS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: June 1, 2002
Last modified: January 7, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Has a deletion of about 30 residues in position 132 compared to all other ThiM.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.