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Q8UAS9 (THIM_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:Atu3288
ORF Names:AGR_L_3060
OrganismAgrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]
Taxonomic identifier176299 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.

Cofactor

Magnesium By similarity.

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1.

Sequence similarities

Belongs to the Thz kinase family.

Caution

Has a deletion of about 30 residues in position 132 compared to all other ThiM.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 246246Probable hydroxyethylthiazole kinase
PRO_0000156923

Sites

Binding site501Substrate; via amide nitrogen By similarity
Binding site1251ATP By similarity
Binding site1451ATP By similarity
Binding site1721Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8UAS9 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 27E5AC411493BCAF

FASTA24626,345
        10         20         30         40         50         60 
MSGDFPTAAR AAEILERVRQ MRPRVHCLMN TVVQKFTADG ITVIGGIPSM TTSLEEIESF 

        70         80         90        100        110        120 
VTKADALTVN LGTLDAERRR VIRLAIEIAN ASGKPWIVDP VHVDYSPSRL DFARELIAQS 

       130        140        150        160        170        180 
PTIVRGNRAE MSLIGDVPDV VRIETGPVDH LRDRTRNVSI VNGHPWMAKV TGTGCLSGGV 

       190        200        210        220        230        240 
IAAFMAVEKD ALTAAASALA VTGVSAELAA QRAKGPGTFE PAFLDALSEI SGEDIINHAR 


IEHEQG 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE007870 Genomic DNA. Translation: AAK90102.1.
PIRAB2961.
D98322.
RefSeqNP_357317.1. NC_003063.2.

3D structure databases

ProteinModelPortalQ8UAS9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176299.Atu3288.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK90102; AAK90102; Atu3288.
GeneID1135162.
KEGGatu:Atu3288.
PATRIC20816034. VBIAgrTum91616_3129.

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMAENLECEQ.
OrthoDBEOG628F8M.

Enzyme and pathway databases

BioCycAGRO:ATU3288-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

Gene3D3.40.1190.20. 2 hits.
InterProIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamPF02110. HK. 2 hits.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
SUPFAMSSF53613. SSF53613. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_AGRT5
AccessionPrimary (citable) accession number: Q8UAS9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways