Reviewed, UniProtKB/Swiss-Prot Q8UAS9 (THIM_AGRT5)

Names and origin

Protein names

Recommended name:
    Probable hydroxyethylthiazole kinase
    EC=2.7.1.50
Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinase
      Short name=Thz kinase
      Short name=TH kinase

Gene names

Name:	thiM
Ordered Locus Names:	Atu3288
ORF Names:	AGR_L_3060

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Pathway	Cofactor biosynthesis; thiamine pyrophosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 4-methyl-5-(2-hydroxyethyl)-thiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.
Caution	Has a deletion of about 30 residues in position 132 compared to all other thiM.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Probable hydroxyethylthiazole kinase HAMAP MF_00228		PRO_0000156923
Sites
	Metal binding	99	1	Magnesium 1 By similarity
	Metal binding	130	1	Magnesium 1 By similarity
	Binding site	50	1	Substrate; via amide nitrogen By similarity
	Binding site	125	1	ATP By similarity
	Binding site	145	1	ATP By similarity
	Binding site	172	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UAS9-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 27E5AC411493BCAF	FASTA	246	26,345
	10 20 30 40 50 60 MSGDFPTAAR AAEILERVRQ MRPRVHCLMN TVVQKFTADG ITVIGGIPSM TTSLEEIESF 70 80 90 100 110 120 VTKADALTVN LGTLDAERRR VIRLAIEIAN ASGKPWIVDP VHVDYSPSRL DFARELIAQS 130 140 150 160 170 180 PTIVRGNRAE MSLIGDVPDV VRIETGPVDH LRDRTRNVSI VNGHPWMAKV TGTGCLSGGV 190 200 210 220 230 240 IAAFMAVEKD ALTAAASALA VTGVSAELAA QRAKGPGTFE PAFLDALSEI SGEDIINHAR IEHEQG

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007870 Genomic DNA. Translation: AAK90102.1.
PIR	AB2961. D98322.
RefSeq	NP_357317.1.
3D structure databases
HSSP	HSSP built from PDB template 1EKQ based on UniProtKB P39593.
ModBase	Search...
Genome annotation databases
GeneID	1135162.
GenomeReviews	Gene locus Atu3288 in contig AE007870_GR.
KEGG	atc:AGR_L_3060. atu:Atu3288.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8UAS9.
OMA	Q8UAS9. ASPVMAH.
Family and domain databases
HAMAP	MF_00228. Divergent sequence. [Tree]
InterPro	IPR000417. Hyethyz_kinase. IPR011144. Hyethyz_kinsmonf. [Graphical view]
PANTHER	PTHR20857:SF14. Hyethyz_kinase. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
ProtoNet	Search...

Entry information

Entry name

THIM_AGRT5

Accession

Primary (citable) accession number: Q8UAS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)