Reviewed, UniProtKB/Swiss-Prot Q8U9X6 (ARLY1_AGRT5)

Names and origin

Protein names

Recommended name:
    Argininosuccinate lyase 1
      Short name=ASAL 1
    EC=4.3.2.1
Alternative name(s):
    Arginosuccinase

Gene names

Name:	argH1
Ordered Locus Names:	Atu3599
ORF Names:	AGR_L_2457

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2-(N(omega)-L-arginino)succinate = fumarate + L-arginine. HAMAP MF_00006
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3. HAMAP MF_00006
Subcellular location	Cytoplasm Probable HAMAP MF_00006.
Sequence similarities	Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process via ornithine Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	argininosuccinate lyase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Argininosuccinate lyase 1 HAMAP MF_00006		PRO_0000137730

Sequences

	Sequence		Length	Mass (Da)
	Q8U9X6-1 [UniParc]. Last modified August 2, 2002. Version 2. Checksum: 507E0EF6C8493F9D	FASTA	466	50,989
	10 20 30 40 50 60 MADGTDQKSS NQMWGGRFAS GPSAIMEEIN ASIGFDKKLY AQDIRGSIAH ATMLAEKGII 70 80 90 100 110 120 SQEDKEKIVH GLNTILSEIE AGTFEFSRKL EDIHMNIEAR LATLIGTAAG RLHTARSRND 130 140 150 160 170 180 QVALDFRLWV KEELQKTEGM LTALIAAFLD RAEENADTVM PGFTHLQTAQ PVTFGHHCMA 190 200 210 220 230 240 YVEMFGRDRA RVRHAIEHLD ESPIGAAALA GTGYPIDRHM TAKALGFREP TRNSIDTVSD 250 260 270 280 290 300 RDFALEFLSI ASICATHLSR LAEEIVIWST PQFGFIRLSD AFSTGSSIMP QKKNPDAAEL 310 320 330 340 350 360 VRAKTGRING SLVALLTVMK GLPLAYSKDM QEDKEQVFDS AESLELAIAA MTGMIRDLEV 370 380 390 400 410 420 RKDRMRAAAG SGYSTATDLA DWLVREAGLP FRDAHHVTGN AVALAEKKGC DLADLSLEEL 430 440 450 460 QAIHPDITNG IFDVLSVEAS VASRTSFGGT APSEVRKQIA WWRGRN

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007870 Genomic DNA. Translation: AAK89798.2.
PIR	AE2999. D98284.
RefSeq	NP_357013.2.
3D structure databases
SMR	Q8U9X6. Positions 22-465.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8U9X6.
Genome annotation databases
GeneID	1135473.
GenomeReviews	Gene locus Atu3599 in contig AE007870_GR.
KEGG	atc:AGR_L_2457. atu:Atu3599.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0165.
HOGENOM	HBG539632.
OMA	ATDTRLY.
PhylomeDB	Q8U9X6.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU3599-MONOMER.
Family and domain databases
HAMAP	MF_00006. Arg_succ_lyase. [Tree]
InterPro	IPR009049. Argininosuccinate_lyase. IPR003031. D_crystallin. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. [Graphical view]
PANTHER	PTHR11444:SF3. argH. 1 hit.
Pfam	PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00838. argH. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARLY1_AGRT5

Accession

Primary (citable) accession number: Q8U9X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	August 2, 2002
Last modified:	February 9, 2010
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)