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Q8U9K4 (RUVC_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:Atu3724
ORF Names:AGR_L_2221
OrganismAgrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]
Taxonomic identifier176299 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

Protein attributes

Sequence length170 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 170170Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_0000183074

Sites

Metal binding111Magnesium By similarity
Metal binding711Magnesium By similarity
Metal binding1431Magnesium By similarity
Metal binding1461Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8U9K4 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: C2965A48D188E051

FASTA17018,166
        10         20         30         40         50         60 
MQKTIRIIGI DPGLRRTGWG VIDTLGNSLR FVASGTVTSD GDMDLASRLC QLHDGLAEIV 

        70         80         90        100        110        120 
HSHQPDEAAV EQTFVNKDAV ATLKLGQARG IAMLVPARAG LPVFEYAPNA VKKAVIGVGH 

       130        140        150        160        170 
GEKQQIHMML KILMPKAEFK GNDAADALAI AICHAHNRGG DRMRRLLAAG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE007870 Genomic DNA. Translation: AAK89681.1.
PIRAH3014.
G98269.
RefSeqNP_356896.1. NC_003063.2.

3D structure databases

ProteinModelPortalQ8U9K4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176299.Atu3724.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK89681; AAK89681; Atu3724.
GeneID1135598.
KEGGatu:Atu3724.
PATRIC20816896. VBIAgrTum91616_3557.

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OMAKTIPHEQ.
OrthoDBEOG6RG044.

Enzyme and pathway databases

BioCycAGRO:ATU3724-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_AGRT5
AccessionPrimary (citable) accession number: Q8U9K4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families