Q8U9I9 (PGK_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase
EC=2.7.2.3

Gene names

Name:	pgk
Ordered Locus Names:	Atu3739
ORF Names:	AGR_L_2193

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium › Agrobacterium tumefaciens complex › Agrobacterium fabrum

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00145
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00145.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Phosphoglycerate kinase HAMAP-Rule MF_00145		PRO_0000145894
Regions
	Nucleotide binding	355 – 358	4	ATP By similarity
	Region	23 – 25	3	Substrate binding By similarity
	Region	61 – 64	4	Substrate binding By similarity
Sites
	Binding site	38	1	Substrate By similarity
	Binding site	120	1	Substrate By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	203	1	ATP By similarity
	Binding site	325	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8U9I9 [UniParc]. Last modified October 25, 2002. Version 2. Checksum: CF4B8BA0C990782F	FASTA	400	41,659
	10 20 30 40 50 60 MPAFKTIDDL NDIAGKRVLV RVDLNVPVAD GKVTDATRIE RVAPTILELS SKGAKVILLA 70 80 90 100 110 120 HFGRPKGEPV AEMSLSQIVP TVEDVLDHAI SFATDCIGAP AADAVAKMND GDILLLENTR 130 140 150 160 170 180 FHKGEEKNDP AFVEELAANG DIYVNDAFSA AHRAHASTEG LARHLPAYAG RTMQAELEAL 190 200 210 220 230 240 EKGLGQPVRP VVAIVGGAKV SSKIDLLMNL VKKVDALVIG GGMANTFLAA RGTNVGKSLC 250 260 270 280 290 300 EHDLAETAKQ IMIEAATSGC AIVLPEDGVV AREFTAGAAN EIVDINAIPA DAMVLDVGPK 310 320 330 340 350 360 SVESIKAWIS RAETLVWNGP LGAFEIEPFD AATVAAAKHA AECTKAGKLV SVAGGGDTVA 370 380 390 400 ALNHAGVSDD FTYISTAGGA FLEWMEGKEL PGVAILTTAK

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007870 Genomic DNA. Translation: AAK89667.2.
PIR	A96268. AG3016.
RefSeq	NP_356882.2. NC_003063.2.
3D structure databases
ProteinModelPortal	Q8U9I9.
ModBase	Search...
Protein-protein interaction databases
STRING	176299.Atu3739.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK89667; AAK89667; Atu3739.
GeneID	1135613.
KEGG	atu:Atu3739.
PATRIC	20816924. VBIAgrTum91616_3571.
Phylogenomic databases
eggNOG	COG0126.
HOGENOM	HOG000227107.
KO	K00927.
OMA	EKKNDEA.
ProtClustDB	PRK00073.
Enzyme and pathway databases
UniPathway	UPA00109; UER00185.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
HAMAP	MF_00145. Phosphoglyc_kinase.
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_AGRT5

Accession

Primary (citable) accession number: Q8U9I9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	October 25, 2002
Last modified:	May 1, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program