Reviewed, UniProtKB/Swiss-Prot Q8U9F7 (HEMH_AGRT5)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	Atu3771
ORF Names:	AGR_L_2129

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Ferrochelatase HAMAP MF_00323		PRO_0000175099
Sites
	Metal binding	214	1	Iron By similarity
	Metal binding	295	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8U9F7-1 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: 33890E7A3B3A0F32	FASTA	344	39,472
	10 20 30 40 50 60 MATELSALPA NHPRVTFGKV GVLLVNLGTP DGTDYWPMRR YLAEFLSDKR VIEWSRLYWY 70 80 90 100 110 120 PILYGIVLNK RPQKVGKAYE EIWNHERNES YLRTYTRSQG ELMAAALKDF PNVVVDWAMR 130 140 150 160 170 180 YGQPSIASRI DALKEQGCEK ILLFPLYPQY AASTTATVND KAFEHLMKLR WQPAIRTVPP 190 200 210 220 230 240 YHDDPAYIEG LAASVKNHLA TLDWEPEMLI TSFHGIPQSY FKKGDPYYCH CQKTARLLRE 250 260 270 280 290 300 ALGRTEKNFM ITFQSRFGPE EWLQPYTDKT VEKLASEGIK RIAVMNPGFV SDCLETLEEI 310 320 330 340 AGEAGEIFLH NGGEKFTHIP CLNDSTEGMN VLEKVVRREL QGWV

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007870 Genomic DNA. Translation: AAK89636.2.
PIR	AG3020. B98264.
RefSeq	NP_356851.2.
3D structure databases
SMR	Q8U9F7. Positions 18-343.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8U9F7.
Genome annotation databases
GeneID	1135645.
GenomeReviews	Gene locus Atu3771 in contig AE007870_GR.
KEGG	atc:AGR_L_2129. atu:Atu3771.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0276.
HOGENOM	HBG697135.
OMA	TIEEIGM.
PhylomeDB	Q8U9F7.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU3771-MONOMER.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_AGRT5

Accession

Primary (citable) accession number: Q8U9F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	August 30, 2002
Last modified:	February 9, 2010
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)