Q8U940 (GLPK_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Atu3890
ORF Names:	AGR_L_1914

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970)

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Glycerol kinase HAMAP MF_00186		PRO_0000059428
Regions
	Nucleotide binding	411 – 415	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	265	1	ATP By similarity
	Binding site	308	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8U940 [UniParc]. Last modified November 1, 2002. Version 2. Checksum: 4A17AE04624E7064	FASTA	499	54,647
	10 20 30 40 50 60 MGGYILAIDQ GTTSTRSMVF DRDMRVIGVG QREFPQHFPA SGWVEHDAED IWKSVLETIR 70 80 90 100 110 120 LALADAGIAA SDIAAIGITN QRETTVLWDR NTGEAVHRAV VWQDRRAAPV CEDLKRRGLE 130 140 150 160 170 180 PLFSKKTGLL LDPYFSGTKL KWLLDSVSGL REKAVKGEIC FGTVDSFLIY RLTGGRRHVT 190 200 210 220 230 240 DATNASRTLI YNIEDNAWDD ELLSILNIPR AMLPEVLDCA ADFGVTDKAL LGAEIPILGV 250 260 270 280 290 300 AGDQQAAVIG NACFEPGMMK STYGTGCFAL LNTGTDRVAS TNRLLTTIAY RLDGVTTYAL 310 320 330 340 350 360 EGSIFIAGAA VQWLRDELGF ISVASEVSAL AEKADPKQRV YLVPAFTGLG APYWDAEARG 370 380 390 400 410 420 AIFGLTRGTG PAEFARAALE SVAYQTFDLL DAMRKDWAGD NGKTVLRVDG GMVASDWTMQ 430 440 450 460 470 480 RLADILAAPV DRPVFLETTI LGAAWLAASR AGIWPDRKAF ADHWRRDRRF SPDMEESERK 490 NAIAGWRDSV GRCLSKRDN

References

[1]	"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.
[2]	"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007870 Genomic DNA. Translation: AAK89529.2.
PIR	AD3035. G98250.
RefSeq	NP_356744.2. NC_003063.2.
3D structure databases
ProteinModelPortal	Q8U940.
SMR	Q8U940. Positions 1-494.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8U940.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1135764.
GenomeReviews	Gene locus Atu3890 in contig AE007870_GR.
KEGG	atu:Atu3890.
PATRIC	20817214. VBIAgrTum91616_3714.
Phylogenomic databases
eggNOG	COG0554.
HOGENOM	HBG511469.
OMA	CYQTRDL.
PhylomeDB	Q8U940.
ProtClustDB	CLSK863056.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU3890-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. False negative. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_AGRT5

Accession

Primary (citable) accession number: Q8U940

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	November 1, 2002
Last modified:	January 25, 2012
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program