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Q8U8L4 (GLGB_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Atu4077
ORF Names:AGR_L_1558
OrganismAgrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]
Taxonomic identifier176299 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

Protein attributes

Sequence length735 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7357351,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188670

Sites

Active site4181Nucleophile By similarity
Active site4711Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8U8L4 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 988721391DC16691

FASTA73583,761
        10         20         30         40         50         60 
MKKPLNSAEE KKTGDITKAE IEAIKSGLHS NPFALLGVHE TPEGFSARCF IPGAEEVSVL 

        70         80         90        100        110        120 
TLDGNFVGEL KQIDPDGFFE GRIDLSKRQP VRYRACRDDA EWAVTDPYSF GPVLGPMDDY 

       130        140        150        160        170        180 
FVREGSHLRL FDRMGAHPLK LEGVEGFHFA VWAPNARRVS VVGDFNNWDG RRHVMRFRKD 

       190        200        210        220        230        240 
TGIWEIFAPD VYAGCAYKFE ILGANGELLP LKADPYARRG ELRPKNASVT APELTQKWED 

       250        260        270        280        290        300 
QAHREHWAQV DQRRQPISIY EVHAGSWQRR EDGTFLSWDE LAAQLIPYCT DMGFTHIEFL 

       310        320        330        340        350        360 
PITEHPYDPS WGYQTTGLYA PTARFGDPEG FARFVNGAHK VGIGVLLDWV PAHFPTDEHG 

       370        380        390        400        410        420 
LRWFDGTALY EHADPRQGFH PDWNTAIYNF GRIEVMSYLI NNALYWAEKF HLDGLRVDAV 

       430        440        450        460        470        480 
ASMLYLDYSR KEGEWIPNEY GGRENLESVR FLQKMNSLVY GTHPGVMTIA EESTSWPKVS 

       490        500        510        520        530        540 
QPVHEGGLGF GFKWNMGFMH DTLSYFSREP VHRKFHHQEL TFGLLYAFTE NFVLPLSHDE 

       550        560        570        580        590        600 
VVHGKGSLIA KMSGDDWQKF ANLRSYYGFM WGYPGKKLLF MGQEFAQWSE WSEKGSLDWN 

       610        620        630        640        650        660 
LRQYPMHEGM RRLVRDLNLT YRSKAALHAR DCEPDGFRWL VVDDHENSVF AWLRTAPGEK 

       670        680        690        700        710        720 
PVAVICNLTP VYRENYYVPL PVAGRWREIL NTDAEIYGGS GKGNGGRVQA VDAGGEIGAM 

       730 
LVLPPLATIM LEPEN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE007870 Genomic DNA. Translation: AAK89352.1.
PIRAH3057.
F98228.
RefSeqNP_356567.1. NC_003063.2.

3D structure databases

ProteinModelPortalQ8U8L4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176299.Atu4077.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK89352; AAK89352; Atu4077.
GeneID1135951.
KEGGatu:Atu4077.
PATRIC20817584. VBIAgrTum91616_3894.

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAWIERINT.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycAGRO:ATU4077-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_AGRT5
AccessionPrimary (citable) accession number: Q8U8L4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries