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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathway: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei418 – 4181NucleophileUniRule annotation
Active sitei471 – 4711Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciAGRO:ATU4077-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:Atu4077
ORF Names:AGR_L_1558
OrganismiAgrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000000813 Componenti: Chromosome linear

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7357351,4-alpha-glucan branching enzyme GlgBPRO_0000188670Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi176299.Atu4077.

Structurei

3D structure databases

ProteinModelPortaliQ8U8L4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDDADHNT.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8U8L4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKPLNSAEE KKTGDITKAE IEAIKSGLHS NPFALLGVHE TPEGFSARCF
60 70 80 90 100
IPGAEEVSVL TLDGNFVGEL KQIDPDGFFE GRIDLSKRQP VRYRACRDDA
110 120 130 140 150
EWAVTDPYSF GPVLGPMDDY FVREGSHLRL FDRMGAHPLK LEGVEGFHFA
160 170 180 190 200
VWAPNARRVS VVGDFNNWDG RRHVMRFRKD TGIWEIFAPD VYAGCAYKFE
210 220 230 240 250
ILGANGELLP LKADPYARRG ELRPKNASVT APELTQKWED QAHREHWAQV
260 270 280 290 300
DQRRQPISIY EVHAGSWQRR EDGTFLSWDE LAAQLIPYCT DMGFTHIEFL
310 320 330 340 350
PITEHPYDPS WGYQTTGLYA PTARFGDPEG FARFVNGAHK VGIGVLLDWV
360 370 380 390 400
PAHFPTDEHG LRWFDGTALY EHADPRQGFH PDWNTAIYNF GRIEVMSYLI
410 420 430 440 450
NNALYWAEKF HLDGLRVDAV ASMLYLDYSR KEGEWIPNEY GGRENLESVR
460 470 480 490 500
FLQKMNSLVY GTHPGVMTIA EESTSWPKVS QPVHEGGLGF GFKWNMGFMH
510 520 530 540 550
DTLSYFSREP VHRKFHHQEL TFGLLYAFTE NFVLPLSHDE VVHGKGSLIA
560 570 580 590 600
KMSGDDWQKF ANLRSYYGFM WGYPGKKLLF MGQEFAQWSE WSEKGSLDWN
610 620 630 640 650
LRQYPMHEGM RRLVRDLNLT YRSKAALHAR DCEPDGFRWL VVDDHENSVF
660 670 680 690 700
AWLRTAPGEK PVAVICNLTP VYRENYYVPL PVAGRWREIL NTDAEIYGGS
710 720 730
GKGNGGRVQA VDAGGEIGAM LVLPPLATIM LEPEN
Length:735
Mass (Da):83,761
Last modified:June 1, 2002 - v1
Checksum:i988721391DC16691
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007870 Genomic DNA. Translation: AAK89352.1.
PIRiAH3057.
F98228.
RefSeqiNP_356567.1. NC_003063.2.
WP_006313779.1. NC_003063.2.

Genome annotation databases

EnsemblBacteriaiAAK89352; AAK89352; Atu4077.
GeneIDi1135951.
KEGGiatu:Atu4077.
PATRICi20817584. VBIAgrTum91616_3894.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007870 Genomic DNA. Translation: AAK89352.1.
PIRiAH3057.
F98228.
RefSeqiNP_356567.1. NC_003063.2.
WP_006313779.1. NC_003063.2.

3D structure databases

ProteinModelPortaliQ8U8L4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176299.Atu4077.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK89352; AAK89352; Atu4077.
GeneIDi1135951.
KEGGiatu:Atu4077.
PATRICi20817584. VBIAgrTum91616_3894.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDDADHNT.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciAGRO:ATU4077-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.

Entry informationi

Entry nameiGLGB_AGRFC
AccessioniPrimary (citable) accession number: Q8U8L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: June 1, 2002
Last modified: June 24, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.