Reviewed, UniProtKB/Swiss-Prot Q8U8J4 (NADB_AGRT5)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:	nadB
Ordered Locus Names:	Atu4097
ORF Names:	AGR_L_1513

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + O2 = iminosuccinate + H2O2.
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 522	522	L-aspartate oxidase		PRO_0000184376
Regions
	Nucleotide binding	12 – 26	15	FAD Potential
Sites
	Active site	232	1	By similarity
	Active site	251	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8U8J4-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 68E3587853650D46	FASTA	522	53,952
	10 20 30 40 50 60 MSEFLPQRDW TVIVGSGMAG LMAAMTLAPQ PVLLVTRGAL GGETSSAWAQ GGIAASLGPD 70 80 90 100 110 120 DRAALHVADT LAAGDGLCDE DMVVGIVSSA PAVIDALERA GVRFDRDAGG NYVFGLEAAH 130 140 150 160 170 180 SRRRILHAEG DGSGAAIVRA LTDAVRRTPS ITVLEGTEVR RLLTEDSVIA GLACAGPNGS 190 200 210 220 230 240 FLLPASQVIL ATGGLGGLYD ATTNPSGNFG QGIMLAARAG AILADMEFVQ FHPTALSSPR 250 260 270 280 290 300 RPLALVSEAV RGEGALLLNE NGERFMAAVP GAELASRDIV ARAIDREILR GGQVFLDARQ 310 320 330 340 350 360 ALGSGFASRF PSIDLLCREA GIDPARELVP VRPAVHYHMG GVATDNKGRS SVRGLWVAGE 370 380 390 400 410 420 TACTGLHGAN RLASNSLLEA AAMGMRAAQD IAGRPAPAAR SAAAVSPNAT ADFSPADLAA 430 440 450 460 470 480 VRPIVSRHLG IVRHAAGLAE AIRDLLPLAE RNGPASDPAV VALSIAVFAA LRRESRGAHF 490 500 510 520 RDDFSQKDAK AMRRRLSLND VVTVAHEFSS SSLATAGFAR SA

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007870 Genomic DNA. Translation: AAK89334.1.
PIR	AD3060. D98226.
RefSeq	NP_356549.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHU based on UniProtKB P10902.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8U8J4.
Genome annotation databases
GeneID	1135971.
GenomeReviews	Gene locus Atu4097 in contig AE007870_GR.
KEGG	atc:AGR_L_1513. atu:Atu4097.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8U8J4.
OMA	GAYIWNR.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR005288. NadB. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00551. nadB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB_AGRT5

Accession

Primary (citable) accession number: Q8U8J4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)