Reviewed, UniProtKB/Swiss-Prot Q8U7P1 (NAPA_AGRT5)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	Atu4408
ORF Names:	AGR_L_917

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	833 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_01630 Binds 1 molybdenum ion per subunit By similarity. HAMAP MF_01630 Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity. HAMAP MF_01630
Subunit structure	Interacts with napB By similarity. HAMAP MF_01630
Subcellular location	Periplasm By similarity HAMAP MF_01630.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Tat-type signal Potential
	Chain	31 – 833	803	Periplasmic nitrate reductase HAMAP MF_01630		PRO_0000045976
Sites
	Metal binding	49	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	52	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	56	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	84	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8U7P1-1 [UniParc]. Last modified February 7, 2006. Version 3. Checksum: 9CE90676A88575B5	FASTA	833	93,414
	10 20 30 40 50 60 MSSELTRRNL KAHAAGIAAA TAGIALPAAA QPVPGGVSAL QIKWSKAPCR FCGTGCGVMV 70 80 90 100 110 120 GVKEGKVVAT HGDMQAEVNR GLNCIKGYFL SKIMYGKDRL ETPLLRKKNG VYAKDGEFEP 130 140 150 160 170 180 VSWDEAFDVM AQQCKRVLKE KGPTAVGMFG SGQWTIFEGY AATKLMRAGF RSNNLDPNAR 190 200 210 220 230 240 HCMASAAYAF MRTFGMDEPM GCYDDFEHAD AFVLWGSNMA EMHPILWTRI ADRRLGFDHV 250 260 270 280 290 300 RVAVLSTFTH RSMDLADIPM VFKPGTDLVI LNYIANHIIK TGRVNEDFVR NHTKFVRGVT 310 320 330 340 350 360 DIGYGLRPDD PVEVNAANSA DPTKTEAIDF ETFKEFVSEY TLEKTAAMTG VEAGFLEELA 370 380 390 400 410 420 ELYADPKRKV MSLWTMGFNQ HVRGVWANQM VYNIHLLTGK ISEPGNSPFS LTGQPSACGT 430 440 450 460 470 480 AREVGTFAHR LPADMTVTNP EHRKHAEEIW RIPHGIIPEK PGYHAVQQDR MLHDGKLNFY 490 500 510 520 530 540 WVQVNNNVQA GPNTKNETYL GYRNPENFIV VSDAYPTITA MSADLILPAA MWVEKEGAYG 550 560 570 580 590 600 NAERRTHVWH QLVEASGEAR SDLWQLVEFS KRFTTDEVWP AEILDANPAY RGKTLYEVLY 610 620 630 640 650 660 KDSDVGKFPL SEINADYKNQ ESSDFGFYLQ KGLFEEYAAF GRGHGHDLAP YDAYHEVRGM 670 680 690 700 710 720 RWPVVDGKET LWRYREGYDP YVKPGEGVKF YGNKDGRAVI IAVPYEPPAE SPDQEFDTWL 730 740 750 760 770 780 VTGRVLEHWH SGSMTMRVPE LYKAFPGARC FMNADDARKR GLNQGAEIRI VSRRGEIRSR 790 800 810 820 830 VETRGRNRMP PGVIFVPWFD ASQLINKVTL DATDPISKQT DFKKCAVKIE PVA

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007870 Genomic DNA. Translation: AAK89031.1.
PIR	AD3098. E98188.
RefSeq	NP_356246.1.
3D structure databases
SMR	Q8U7P1. Positions 42-830.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8U7P1.
Genome annotation databases
GeneID	1136282.
GenomeReviews	Gene locus Atu4408 in contig AE007870_GR.
KEGG	atc:AGR_L_917. atu:Atu4408.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HBG715033.
OMA	QFWHQLV.
PhylomeDB	Q8U7P1.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU4408-MONOMER.
Family and domain databases
HAMAP	MF_01630. Nitrate_reduct. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_AGRT5

Accession

Primary (citable) accession number: Q8U7P1
Secondary accession number(s): Q7CUV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	February 7, 2006
Last modified:	February 9, 2010
This is version 49 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)