Q8U7P1 (NAPA_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Periplasmic nitrate reductase
EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	Atu4408
ORF Names:	AGR_L_917

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium › Agrobacterium tumefaciens complex › Agrobacterium fabrum

Protein attributes

Sequence length	833 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with NapB By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Tat-type signal Potential
	Chain	31 – 833	803	Periplasmic nitrate reductase HAMAP-Rule MF_01630		PRO_0000045976
Sites
	Metal binding	49	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	52	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	56	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	84	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8U7P1 [UniParc]. Last modified February 7, 2006. Version 3. Checksum: 9CE90676A88575B5	FASTA	833	93,414
	10 20 30 40 50 60 MSSELTRRNL KAHAAGIAAA TAGIALPAAA QPVPGGVSAL QIKWSKAPCR FCGTGCGVMV 70 80 90 100 110 120 GVKEGKVVAT HGDMQAEVNR GLNCIKGYFL SKIMYGKDRL ETPLLRKKNG VYAKDGEFEP 130 140 150 160 170 180 VSWDEAFDVM AQQCKRVLKE KGPTAVGMFG SGQWTIFEGY AATKLMRAGF RSNNLDPNAR 190 200 210 220 230 240 HCMASAAYAF MRTFGMDEPM GCYDDFEHAD AFVLWGSNMA EMHPILWTRI ADRRLGFDHV 250 260 270 280 290 300 RVAVLSTFTH RSMDLADIPM VFKPGTDLVI LNYIANHIIK TGRVNEDFVR NHTKFVRGVT 310 320 330 340 350 360 DIGYGLRPDD PVEVNAANSA DPTKTEAIDF ETFKEFVSEY TLEKTAAMTG VEAGFLEELA 370 380 390 400 410 420 ELYADPKRKV MSLWTMGFNQ HVRGVWANQM VYNIHLLTGK ISEPGNSPFS LTGQPSACGT 430 440 450 460 470 480 AREVGTFAHR LPADMTVTNP EHRKHAEEIW RIPHGIIPEK PGYHAVQQDR MLHDGKLNFY 490 500 510 520 530 540 WVQVNNNVQA GPNTKNETYL GYRNPENFIV VSDAYPTITA MSADLILPAA MWVEKEGAYG 550 560 570 580 590 600 NAERRTHVWH QLVEASGEAR SDLWQLVEFS KRFTTDEVWP AEILDANPAY RGKTLYEVLY 610 620 630 640 650 660 KDSDVGKFPL SEINADYKNQ ESSDFGFYLQ KGLFEEYAAF GRGHGHDLAP YDAYHEVRGM 670 680 690 700 710 720 RWPVVDGKET LWRYREGYDP YVKPGEGVKF YGNKDGRAVI IAVPYEPPAE SPDQEFDTWL 730 740 750 760 770 780 VTGRVLEHWH SGSMTMRVPE LYKAFPGARC FMNADDARKR GLNQGAEIRI VSRRGEIRSR 790 800 810 820 830 VETRGRNRMP PGVIFVPWFD ASQLINKVTL DATDPISKQT DFKKCAVKIE PVA

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007870 Genomic DNA. Translation: AAK89031.1.
PIR	AD3098. E98188.
RefSeq	NP_356246.1. NC_003063.2.
3D structure databases
ProteinModelPortal	Q8U7P1.
SMR	Q8U7P1. Positions 42-830.
ModBase	Search...
Protein-protein interaction databases
STRING	176299.Atu4408.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK89031; AAK89031; Atu4408.
GeneID	1136282.
KEGG	atu:Atu4408.
PATRIC	20818241. VBIAgrTum91616_4216.
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HOG000031441.
KO	K02567.
OMA	NAYWVQV.
ProtClustDB	PRK13532.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
HAMAP	MF_01630. Nitrate_reduct.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. Periplasm_NO3_reductase_lsu. IPR006311. TAT_signal. [Graphical view]
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR01706. NAPA. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_AGRT5

Accession

Primary (citable) accession number: Q8U7P1
Secondary accession number(s): Q7CUV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	February 7, 2006
Last modified:	May 1, 2013
This is version 70 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program