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Q8U7P1

- NAPA_AGRT5

UniProt

Q8U7P1 - NAPA_AGRT5

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 4 (16 Apr 2014)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi57 – 571Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi85 – 851Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:Atu4408
    ORF Names:AGR_L_917
    OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
    Taxonomic identifieri176299 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
    ProteomesiUP000000813: Chromosome linear

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
    BLAST
    Chaini32 – 834803Periplasmic nitrate reductasePRO_0000045976Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi176299.Atu4408.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8U7P1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 99574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiHQLVEAP.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8U7P1-1 [UniParc]FASTAAdd to Basket

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    MSSELTRRNL LKAHAAGIAA ATAGIALPAA AQPVPGGVSA LQIKWSKAPC    50
    RFCGTGCGVM VGVKEGKVVA THGDMQAEVN RGLNCIKGYF LSKIMYGKDR 100
    LETPLLRKKN GVYAKDGEFE PVSWDEAFDV MAQQCKRVLK EKGPTAVGMF 150
    GSGQWTIFEG YAATKLMRAG FRSNNLDPNA RHCMASAAYA FMRTFGMDEP 200
    MGCYDDFEHA DAFVLWGSNM AEMHPILWTR IADRRLGFDH VRVAVLSTFT 250
    HRSMDLADIP MVFKPGTDLV ILNYIANHII KTGRVNEDFV RNHTKFVRGV 300
    TDIGYGLRPD DPVEVNAANS ADPTKTEAID FETFKEFVSE YTLEKTAAMT 350
    GVEAGFLEEL AELYADPKRK VMSLWTMGFN QHVRGVWANQ MVYNIHLLTG 400
    KISEPGNSPF SLTGQPSACG TAREVGTFAH RLPADMTVTN PEHRKHAEEI 450
    WRIPHGIIPE KPGYHAVQQD RMLHDGKLNF YWVQVNNNVQ AGPNTKNETY 500
    LGYRNPENFI VVSDAYPTIT AMSADLILPA AMWVEKEGAY GNAERRTHVW 550
    HQLVEASGEA RSDLWQLVEF SKRFTTDEVW PAEILDANPA YRGKTLYEVL 600
    YKDSDVGKFP LSEINADYKN QESSDFGFYL QKGLFEEYAA FGRGHGHDLA 650
    PYDAYHEVRG MRWPVVDGKE TLWRYREGYD PYVKPGEGVK FYGNKDGRAV 700
    IIAVPYEPPA ESPDQEFDTW LVTGRVLEHW HSGSMTMRVP ELYKAFPGAR 750
    CFMNADDARK RGLNQGAEIR IVSRRGEIRS RVETRGRNRM PPGVIFVPWF 800
    DASQLINKVT LDATDPISKQ TDFKKCAVKI EPVA 834
    Length:834
    Mass (Da):93,527
    Last modified:April 16, 2014 - v4
    Checksum:iFF707BC71CA08B9E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE007870 Genomic DNA. Translation: AAK89031.1.
    PIRiAD3098.
    E98188.
    RefSeqiNP_356246.1. NC_003063.2.

    Genome annotation databases

    EnsemblBacteriaiAAK89031; AAK89031; Atu4408.
    GeneIDi1136282.
    KEGGiatu:Atu4408.
    PATRICi20818241. VBIAgrTum91616_4216.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE007870 Genomic DNA. Translation: AAK89031.1 .
    PIRi AD3098.
    E98188.
    RefSeqi NP_356246.1. NC_003063.2.

    3D structure databases

    ProteinModelPortali Q8U7P1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 176299.Atu4408.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK89031 ; AAK89031 ; Atu4408 .
    GeneIDi 1136282.
    KEGGi atu:Atu4408.
    PATRICi 20818241. VBIAgrTum91616_4216.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi HQLVEAP.
    OrthoDBi EOG6CVV7G.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C58 / ATCC 33970.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C58 / ATCC 33970.

    Entry informationi

    Entry nameiNAPA_AGRT5
    AccessioniPrimary (citable) accession number: Q8U7P1
    Secondary accession number(s): Q7CUV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: April 16, 2014
    Last modified: October 1, 2014
    This is version 79 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3