Reviewed,
UniProtKB/Swiss-Prot Q8U7P1 (NAPA_AGRT5)
Last modified
February 9, 2010.
Version 49.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Periplasmic nitrate reductase EC=1.7.99.4 | ||||||
| Gene names |
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| Organism | Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 176299 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium |
Protein attributes
| Sequence length | 833 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP MF_01630 |
| Catalytic activity | Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630 |
| Cofactor | Binds 1 4Fe-4S cluster By similarity. HAMAP MF_01630 Binds 1 molybdenum ion per subunit By similarity. HAMAP MF_01630 Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity. HAMAP MF_01630 |
| Subunit structure | Interacts with napB By similarity. HAMAP MF_01630 |
| Subcellular location | Periplasm By similarity HAMAP MF_01630. |
| Post-translational modification | Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_01630 |
| Sequence similarities | Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 30 | 30 | Tat-type signal Potential | ||||||
| Chain | 31 – 833 | 803 | Periplasmic nitrate reductase HAMAP MF_01630 | PRO_0000045976 | |||||
Sites | |||||||||
| Metal binding | 49 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 52 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 56 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 84 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
Sequences
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References
| [1] | "The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. Nester E.W.Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [2] | "Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. Slater S.Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE007870 Genomic DNA. Translation: AAK89031.1. |
| PIR | AD3098. E98188. |
| RefSeq | NP_356246.1. |
3D structure databases | |
| SMR | Q8U7P1. Positions 42-830. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q8U7P1. |
Genome annotation databases | |
| GeneID | 1136282. |
| GenomeReviews | Gene locus Atu4408 in contig AE007870_GR. |
| KEGG | atc:AGR_L_917. atu:Atu4408. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | COG0243. |
| HOGENOM | HBG715033. |
| OMA | QFWHQLV. |
| PhylomeDB | Q8U7P1. |
Enzyme and pathway databases | |
| BioCyc | ATUM176299-1:ATU4408-MONOMER. |
Family and domain databases | |
| HAMAP | MF_01630. Nitrate_reduct. [Tree] |
| InterPro | IPR009010. Asp_de-COase-like_fold. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view] |
| Gene3D | G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit. |
| Pfam | PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit. |
| PROSITE | PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NAPA_AGRT5 | ||||||||
| Accession | Primary (citable) accession number: Q8U7P1 Secondary accession number(s): Q7CUV7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||

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