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Q8U7P1 (NAPA_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:Atu4408
ORF Names:AGR_L_917
OrganismAgrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]
Taxonomic identifier176299 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

Protein attributes

Sequence length834 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131Tat-type signal Potential
Chain32 – 834803Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000045976

Regions

Domain43 – 99574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding501Iron-sulfur (4Fe-4S) By similarity
Metal binding531Iron-sulfur (4Fe-4S) By similarity
Metal binding571Iron-sulfur (4Fe-4S) By similarity
Metal binding851Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8U7P1 [UniParc].

Last modified April 16, 2014. Version 4.
Checksum: FF707BC71CA08B9E

FASTA83493,527
        10         20         30         40         50         60 
MSSELTRRNL LKAHAAGIAA ATAGIALPAA AQPVPGGVSA LQIKWSKAPC RFCGTGCGVM 

        70         80         90        100        110        120 
VGVKEGKVVA THGDMQAEVN RGLNCIKGYF LSKIMYGKDR LETPLLRKKN GVYAKDGEFE 

       130        140        150        160        170        180 
PVSWDEAFDV MAQQCKRVLK EKGPTAVGMF GSGQWTIFEG YAATKLMRAG FRSNNLDPNA 

       190        200        210        220        230        240 
RHCMASAAYA FMRTFGMDEP MGCYDDFEHA DAFVLWGSNM AEMHPILWTR IADRRLGFDH 

       250        260        270        280        290        300 
VRVAVLSTFT HRSMDLADIP MVFKPGTDLV ILNYIANHII KTGRVNEDFV RNHTKFVRGV 

       310        320        330        340        350        360 
TDIGYGLRPD DPVEVNAANS ADPTKTEAID FETFKEFVSE YTLEKTAAMT GVEAGFLEEL 

       370        380        390        400        410        420 
AELYADPKRK VMSLWTMGFN QHVRGVWANQ MVYNIHLLTG KISEPGNSPF SLTGQPSACG 

       430        440        450        460        470        480 
TAREVGTFAH RLPADMTVTN PEHRKHAEEI WRIPHGIIPE KPGYHAVQQD RMLHDGKLNF 

       490        500        510        520        530        540 
YWVQVNNNVQ AGPNTKNETY LGYRNPENFI VVSDAYPTIT AMSADLILPA AMWVEKEGAY 

       550        560        570        580        590        600 
GNAERRTHVW HQLVEASGEA RSDLWQLVEF SKRFTTDEVW PAEILDANPA YRGKTLYEVL 

       610        620        630        640        650        660 
YKDSDVGKFP LSEINADYKN QESSDFGFYL QKGLFEEYAA FGRGHGHDLA PYDAYHEVRG 

       670        680        690        700        710        720 
MRWPVVDGKE TLWRYREGYD PYVKPGEGVK FYGNKDGRAV IIAVPYEPPA ESPDQEFDTW 

       730        740        750        760        770        780 
LVTGRVLEHW HSGSMTMRVP ELYKAFPGAR CFMNADDARK RGLNQGAEIR IVSRRGEIRS 

       790        800        810        820        830 
RVETRGRNRM PPGVIFVPWF DASQLINKVT LDATDPISKQ TDFKKCAVKI EPVA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE007870 Genomic DNA. Translation: AAK89031.1.
PIRAD3098.
E98188.
RefSeqNP_356246.1. NC_003063.2.

3D structure databases

ProteinModelPortalQ8U7P1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176299.Atu4408.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK89031; AAK89031; Atu4408.
GeneID1136282.
KEGGatu:Atu4408.
PATRIC20818241. VBIAgrTum91616_4216.

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAHQLVEAP.
OrthoDBEOG6CVV7G.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_AGRT5
AccessionPrimary (citable) accession number: Q8U7P1
Secondary accession number(s): Q7CUV7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: April 16, 2014
Last modified: May 14, 2014
This is version 78 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families