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Q8U7P1

- NAPA_AGRT5

UniProt

Q8U7P1 - NAPA_AGRT5

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Protein

Periplasmic nitrate reductase

Gene
napA, Atu4408, AGR_L_917
Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi50 – 501Iron-sulfur (4Fe-4S) By similarity
Metal bindingi53 – 531Iron-sulfur (4Fe-4S) By similarity
Metal bindingi57 – 571Iron-sulfur (4Fe-4S) By similarity
Metal bindingi85 – 851Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:Atu4408
ORF Names:AGR_L_917
OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000000813: Chromosome linear

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signal Reviewed predictionAdd
BLAST
Chaini32 – 834803Periplasmic nitrate reductaseUniRule annotationPRO_0000045976Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi176299.Atu4408.

Structurei

3D structure databases

ProteinModelPortaliQ8U7P1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 99574Fe-4S Mo/W bis-MGD-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiHQLVEAP.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8U7P1-1 [UniParc]FASTAAdd to Basket

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MSSELTRRNL LKAHAAGIAA ATAGIALPAA AQPVPGGVSA LQIKWSKAPC    50
RFCGTGCGVM VGVKEGKVVA THGDMQAEVN RGLNCIKGYF LSKIMYGKDR 100
LETPLLRKKN GVYAKDGEFE PVSWDEAFDV MAQQCKRVLK EKGPTAVGMF 150
GSGQWTIFEG YAATKLMRAG FRSNNLDPNA RHCMASAAYA FMRTFGMDEP 200
MGCYDDFEHA DAFVLWGSNM AEMHPILWTR IADRRLGFDH VRVAVLSTFT 250
HRSMDLADIP MVFKPGTDLV ILNYIANHII KTGRVNEDFV RNHTKFVRGV 300
TDIGYGLRPD DPVEVNAANS ADPTKTEAID FETFKEFVSE YTLEKTAAMT 350
GVEAGFLEEL AELYADPKRK VMSLWTMGFN QHVRGVWANQ MVYNIHLLTG 400
KISEPGNSPF SLTGQPSACG TAREVGTFAH RLPADMTVTN PEHRKHAEEI 450
WRIPHGIIPE KPGYHAVQQD RMLHDGKLNF YWVQVNNNVQ AGPNTKNETY 500
LGYRNPENFI VVSDAYPTIT AMSADLILPA AMWVEKEGAY GNAERRTHVW 550
HQLVEASGEA RSDLWQLVEF SKRFTTDEVW PAEILDANPA YRGKTLYEVL 600
YKDSDVGKFP LSEINADYKN QESSDFGFYL QKGLFEEYAA FGRGHGHDLA 650
PYDAYHEVRG MRWPVVDGKE TLWRYREGYD PYVKPGEGVK FYGNKDGRAV 700
IIAVPYEPPA ESPDQEFDTW LVTGRVLEHW HSGSMTMRVP ELYKAFPGAR 750
CFMNADDARK RGLNQGAEIR IVSRRGEIRS RVETRGRNRM PPGVIFVPWF 800
DASQLINKVT LDATDPISKQ TDFKKCAVKI EPVA 834
Length:834
Mass (Da):93,527
Last modified:April 16, 2014 - v4
Checksum:iFF707BC71CA08B9E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007870 Genomic DNA. Translation: AAK89031.1.
PIRiAD3098.
E98188.
RefSeqiNP_356246.1. NC_003063.2.

Genome annotation databases

EnsemblBacteriaiAAK89031; AAK89031; Atu4408.
GeneIDi1136282.
KEGGiatu:Atu4408.
PATRICi20818241. VBIAgrTum91616_4216.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007870 Genomic DNA. Translation: AAK89031.1 .
PIRi AD3098.
E98188.
RefSeqi NP_356246.1. NC_003063.2.

3D structure databases

ProteinModelPortali Q8U7P1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 176299.Atu4408.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK89031 ; AAK89031 ; Atu4408 .
GeneIDi 1136282.
KEGGi atu:Atu4408.
PATRICi 20818241. VBIAgrTum91616_4216.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi HQLVEAP.
OrthoDBi EOG6CVV7G.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.

Entry informationi

Entry nameiNAPA_AGRT5
AccessioniPrimary (citable) accession number: Q8U7P1
Secondary accession number(s): Q7CUV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: April 16, 2014
Last modified: May 14, 2014
This is version 78 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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