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Q8U7H5 (SYE_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:Atu4474
ORF Names:AGR_L_791
OrganismAgrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]
Taxonomic identifier176299 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

Protein attributes

Sequence length485 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 485485Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000119491

Regions

Motif12 – 2211"HIGH" region HAMAP-Rule MF_00022
Motif253 – 2575"KMSKS" region HAMAP-Rule MF_00022

Sites

Metal binding1091Zinc By similarity
Metal binding1111Zinc By similarity
Metal binding1361Zinc By similarity
Metal binding1381Zinc By similarity
Binding site2561ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8U7H5 [UniParc].

Last modified April 30, 2003. Version 2.
Checksum: 8E74D02F7F383660

FASTA48554,228
        10         20         30         40         50         60 
MTTSGVRVRI APSPTGEPHV GTAYIALFNY LFAKKHGGEF ILRIEDTDAT RSTLEYEQKV 

        70         80         90        100        110        120 
LEALRWTGLT WSEGPDVGGP YGPYRQSERK PMYWPYAEEL LEKGHAFRCF CTPERLEQMR 

       130        140        150        160        170        180 
EAQRAAGKPP KYDGLCLHLK AEEVTTRVAA GEANVVRMKI PTEGSCDFHD GVYGDVSIPW 

       190        200        210        220        230        240 
DSVDMQVLIK ADGMPTYHMA NVIDDHLMKI THVARGEEWL ASVPKHILLY RYFGWDQPTF 

       250        260        270        280        290        300 
MHLSLMRNAD KSKLSKRKNP TSISYYSALG YLPEALMNFL GLFFIQIAEG EELLTMEELA 

       310        320        330        340        350        360 
AKFDPEALSK AGAIFDIQKL DWLNGRWLRE KLTPEDFIAR TLEWAMENSR LTEGLKLAQS 

       370        380        390        400        410        420 
RISKLGELPN LAGFLVSSDV GLTPASFAGL KSKPEEIHEI LTTVAADLEK MPDWNVEAIE 

       430        440        450        460        470        480 
AELRDIAERT GKKLRVVTPP LFVAVSGSSR SLPLFDSMAL LGRSVVRQRL KVAIAVVATM 


VGSGS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE007870 Genomic DNA. Translation: AAK88968.2.
PIRAF3106.
F98180.
RefSeqNP_356183.2. NC_003063.2.

3D structure databases

ProteinModelPortalQ8U7H5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176299.Atu4474.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK88968; AAK88968; Atu4474.
GeneID1136348.
KEGGatu:Atu4474.
PATRIC20818373. VBIAgrTum91616_4282.

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK01885.
OMAHCLRASI.
OrthoDBEOG6DRPF7.

Enzyme and pathway databases

BioCycAGRO:ATU4474-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_AGRT5
AccessionPrimary (citable) accession number: Q8U7H5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: May 14, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries