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Q8U7H5

- SYE_AGRT5

UniProt

Q8U7H5 - SYE_AGRT5

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Protein

Glutamate--tRNA ligase

Gene
gltX, Atu4474, AGR_L_791
Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi109 – 1091Zinc By similarity
Metal bindingi111 – 1111Zinc By similarity
Metal bindingi136 – 1361Zinc By similarity
Metal bindingi138 – 1381Zinc By similarity
Binding sitei256 – 2561ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAGRO:ATU4474-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:gltX
Ordered Locus Names:Atu4474
ORF Names:AGR_L_791
OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000000813: Chromosome linear

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485Glutamate--tRNA ligaseUniRule annotationPRO_0000119491Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi176299.Atu4474.

Structurei

3D structure databases

ProteinModelPortaliQ8U7H5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 2211"HIGH" regionUniRule annotationAdd
BLAST
Motifi253 – 2575"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiHCLRASI.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U7H5-1 [UniParc]FASTAAdd to Basket

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MTTSGVRVRI APSPTGEPHV GTAYIALFNY LFAKKHGGEF ILRIEDTDAT    50
RSTLEYEQKV LEALRWTGLT WSEGPDVGGP YGPYRQSERK PMYWPYAEEL 100
LEKGHAFRCF CTPERLEQMR EAQRAAGKPP KYDGLCLHLK AEEVTTRVAA 150
GEANVVRMKI PTEGSCDFHD GVYGDVSIPW DSVDMQVLIK ADGMPTYHMA 200
NVIDDHLMKI THVARGEEWL ASVPKHILLY RYFGWDQPTF MHLSLMRNAD 250
KSKLSKRKNP TSISYYSALG YLPEALMNFL GLFFIQIAEG EELLTMEELA 300
AKFDPEALSK AGAIFDIQKL DWLNGRWLRE KLTPEDFIAR TLEWAMENSR 350
LTEGLKLAQS RISKLGELPN LAGFLVSSDV GLTPASFAGL KSKPEEIHEI 400
LTTVAADLEK MPDWNVEAIE AELRDIAERT GKKLRVVTPP LFVAVSGSSR 450
SLPLFDSMAL LGRSVVRQRL KVAIAVVATM VGSGS 485
Length:485
Mass (Da):54,228
Last modified:April 30, 2003 - v2
Checksum:i8E74D02F7F383660
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007870 Genomic DNA. Translation: AAK88968.2.
PIRiAF3106.
F98180.
RefSeqiNP_356183.2. NC_003063.2.

Genome annotation databases

EnsemblBacteriaiAAK88968; AAK88968; Atu4474.
GeneIDi1136348.
KEGGiatu:Atu4474.
PATRICi20818373. VBIAgrTum91616_4282.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007870 Genomic DNA. Translation: AAK88968.2 .
PIRi AF3106.
F98180.
RefSeqi NP_356183.2. NC_003063.2.

3D structure databases

ProteinModelPortali Q8U7H5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 176299.Atu4474.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK88968 ; AAK88968 ; Atu4474 .
GeneIDi 1136348.
KEGGi atu:Atu4474.
PATRICi 20818373. VBIAgrTum91616_4282.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252720.
KOi K01885.
OMAi HCLRASI.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci AGRO:ATU4474-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.

Entry informationi

Entry nameiSYE_AGRT5
AccessioniPrimary (citable) accession number: Q8U7H5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: May 14, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi