Reviewed, UniProtKB/Swiss-Prot Q8U7G6 (XYLA_AGRT5)

Names and origin

Protein names

Recommended name:
    Xylose isomerase
    EC=5.3.1.5

Gene names

Name:	xylA
Ordered Locus Names:	Atu4483
ORF Names:	AGR_L_774

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-xylose = D-xylulose. HAMAP MF_00455
Cofactor	Binds 2 magnesium ions per subunit By similarity. HAMAP MF_00455
Subunit structure	Homotetramer By similarity. HAMAP MF_00455
Subcellular location	Cytoplasm By similarity HAMAP MF_00455.
Sequence similarities	Belongs to the xylose isomerase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Pentose shunt Xylose metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	D-xylose metabolic process Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP xylose isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	Xylose isomerase HAMAP MF_00455		PRO_0000195758
Sites
	Active site	100	1	By similarity
	Active site	103	1	By similarity
	Metal binding	231	1	Magnesium 1 By similarity
	Metal binding	267	1	Magnesium 1 By similarity
	Metal binding	267	1	Magnesium 2 By similarity
	Metal binding	270	1	Magnesium 2 By similarity
	Metal binding	295	1	Magnesium 1 By similarity
	Metal binding	306	1	Magnesium 2 By similarity
	Metal binding	308	1	Magnesium 2 By similarity
	Metal binding	338	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8U7G6-1 [UniParc]. Last modified July 5, 2004. Version 2. Checksum: 5B8759CF40D9F2E1	FASTA	436	48,968
	10 20 30 40 50 60 MSTGFFGDIA KIKYEGPDST NPLAFRHYNP DEIVGGKRME DHLRFAVAYW HTFTWPGGDP 70 80 90 100 110 120 FGGQTFQRPW FEDTMQAAKL KADVAFEFFS LLGSPFYCFH DADVRPEGKN FAENTKNLNE 130 140 150 160 170 180 IVDYFAQKQA DTGVKLLWGT ANLFSNRRFM SGAATNPDPD VFAFSAATVK TCMDATKTLG 190 200 210 220 230 240 GANYVLWGGR EGYETLLNTD LSRELDQLGR FLNLVVEYKY KIGFEGTILI EPKPQEPTKH 250 260 270 280 290 300 QYDYDVATVY AFLQKNGLEK EVKVNIEQGH AILAGHSFEH ELAMANAFGI FGSIDMNRND 310 320 330 340 350 360 YQSGWDTDQF PNNVPEMALA YYHVLAGGGF KNGGTNFDSK LRRQSLDPQD LLIGHIGGMD 370 380 390 400 410 420 CCARGLKAAA KMIEDGALSK PLSERYAKWD SPEAQKMLRG ELKLEEIAAL VERDDINPEP 430 KPGRQEYLEN VVNRYV

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007870 Genomic DNA. Translation: AAK88959.2.
PIR	AG3107. E98179.
RefSeq	NP_356174.2.
3D structure databases
SMR	Q8U7G6. Positions 4-436.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8U7G6.
Genome annotation databases
GeneID	1136357.
GenomeReviews	Gene locus Atu4483 in contig AE007870_GR.
KEGG	atc:AGR_L_774. atu:Atu4483.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2115.
HOGENOM	HBG297199.
OMA	LLGWDTD.
PhylomeDB	Q8U7G6.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU4483-MONOMER.
Family and domain databases
HAMAP	MF_00455. Xylose_isom_A. [Tree]
InterPro	IPR013022. Xyl_isomerase-like_TIM-brl. IPR012307. Xyl_isomerase_TIM-brl. IPR013452. Xylose_isom_bac. IPR001998. Xylose_isomerase. [Graphical view]
Gene3D	G3DSA:3.20.20.150. Xyl_isomerase-like_TIM-brl. 1 hit.
Pfam	PF01261. AP_endonuc_2. 1 hit. [Graphical view]
PRINTS	PR00688. XYLOSISMRASE.
TIGRFAMs	TIGR02630. xylose_isom_A. 1 hit.
PROSITE	PS51415. XYLOSE_ISOMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLA_AGRT5

Accession

Primary (citable) accession number: Q8U7G6
Secondary accession number(s): Q7CV26

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)