Reviewed, UniProtKB/Swiss-Prot Q8U642 (DNE23_AGRT5)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase 3
    EC=2.7.7.7

Gene names

Name:	dnaE2-3
Ordered Locus Names:	Atu6093
ORF Names:	AGR_pTi_175

Encoded on

Plasmid pTiC58

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	1087 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1087	1087	Error-prone DNA polymerase 3 HAMAP MF_01902		PRO_0000103364

Sequences

	Sequence		Length	Mass (Da)
	Q8U642-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 66C81289A3841EC6	FASTA	1,087	122,156
	10 20 30 40 50 60 MSYAELQVTT HFSFLRGASS AQELFETAKA LGIEALGVVD RNSLAGIVRA LEASRATGLR 70 80 90 100 110 120 LVIGCRLDLA DGMSVLVYPT DRAAYSRLTR LITLGKSRGG KNNCLLHWDD VVAYTDGMIG 130 140 150 160 170 180 ILVPDLPDDL CGIQLRKMAE LFGDRAYVSL CLRRRQNDQL RLHEISNLAT RFKVRTVVTN 190 200 210 220 230 240 DVLFHEPGRR QLQDIVTCIR TRTTIDEVGF ERERHADRYL KPPEEMERLF PRYRQALART 250 260 270 280 290 300 MEIVRRCTFS LEELTYQYPE EAIVPGKDAQ ASLEHYVWQC VPDRYPEGLP PDVLKVVRHE 310 320 330 340 350 360 LDLIRTMKYA PYFLTVFSIV RYARSQGILC QGRGSAANSA VCYILGITSI DPSTNDLLFE 370 380 390 400 410 420 RFVSQERDEP PDIDVDFEHE RREEVIQWIY RTYTREKAAL CATVTRYRAR GAIRDVGKAL 430 440 450 460 470 480 GLPEDVIKAL SSGMWSWSEE VCDRNVRELN LNPDDRRLVL TLKLAQQLMG APRHLGQHPG 490 500 510 520 530 540 GFVLTHDRLD DLVPIEPATM KDRQIIEWDK DDVEALKFMK VDILALGMLT CMAKAFDLIR 550 560 570 580 590 600 EHKDRDLDLS KIEQEDSVTY AMIRKADTLG TFQIESRAQM AMLPRLKPRT FYDLVVQVAI 610 620 630 640 650 660 VRPGPIQGDM VHPYLRRREG KEAVEYPTPE LEAVLGKTLG VPLFQESAMR VAMVCAGFTG 670 680 690 700 710 720 GEADQLRKSM ATFKFTGGVS QFKDKLVSGM VRNGYAPEFA EKTFSQLEGF GSYGFPESHA 730 740 750 760 770 780 ASFALIAYAS SYIKCHYPEA FCAALINSQP MGFYAPAQIV GDARAHGVEV RPVCINRSRW 790 800 810 820 830 840 DCTLERIGNS DRHAVRLGFR QVKGLAVADA ARVVAARMNN AFVSVDDMWR RSGVPSEALV 850 860 870 880 890 900 QLAKADAFLP SLKLERRDAL WAIKALRDEP LPLFAAAAER EMAAIAEQQE PGVALRQMTD 910 920 930 940 950 960 GHNVIEDYSH TGLTLRQHPI AFLRKDLSVR NIITCAEAMN SRDGRWVYTA GLVLVRQKPG 970 980 990 1000 1010 1020 SAKGVMFITI EDETGPANLV VWPTLFEKRR RAVLGSSMMA INGRIQREGE VVHLVAQQLF 1030 1040 1050 1060 1070 1080 DLSGDLTGLA DRDEEFKLPA GRGDEFAHGS PGSSDTRDKS KPVVAPRDIF TPDLHIDTLK IKSRNFH

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007871 Genomic DNA. Translation: AAK91053.1.
PIR	AC3239.
RefSeq	NP_396612.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1137416.
GenomeReviews	Gene locus Atu6093 in contig AE007871_GR.
KEGG	atc:AGR_pTi_175. atu:Atu6093.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8U642.
OMA	Q8U642. TRIVEAR.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. PHP_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNE23_AGRT5

Accession

Primary (citable) accession number: Q8U642
Secondary accession number(s): Q7D2J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)