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Protein

DNA translocase FtsK

Gene

ftsK

Organism
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi544 – 5496ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAGRO:ATU2759-MONOMER.
RETL1328306-WGS:GSTH-4060-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA translocase FtsK
Gene namesi
Name:ftsK
Ordered Locus Names:Atu2759
ORF Names:AGR_C_5003
OrganismiAgrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
Proteomesi
  • UP000000813 Componenti: Chromosome circular

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei26 – 4621HelicalSequence analysisAdd
BLAST
Transmembranei81 – 10121HelicalSequence analysisAdd
BLAST
Transmembranei114 – 13421HelicalSequence analysisAdd
BLAST
Transmembranei141 – 16121HelicalSequence analysisAdd
BLAST
Transmembranei169 – 18921HelicalSequence analysisAdd
BLAST
Transmembranei225 – 24521HelicalSequence analysisAdd
BLAST
Topological domaini246 – 891646CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 891891DNA translocase FtsKPRO_0000098234Add
BLAST

Interactioni

Subunit structurei

Homohexamer. Forms a ring that surrounds DNA (By similarity).By similarity

Protein-protein interaction databases

STRINGi176299.Atu2759.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini524 – 743220FtsKPROSITE-ProRule annotationAdd
BLAST

Domaini

Consists of an N-terminal domain, which is sufficient for the localization to the septal ring and is required for cell division, followed by a linker domain, and a C-terminal domain, which forms the translocation motor involved in chromosome segregation. The C-terminal domain can be further subdivided into alpha, beta and gamma subdomains. The alpha and beta subdomains multimerise to produce a hexameric ring, contain the nucleotide binding motif and form the DNA pump. The gamma subdomain is a regulatory subdomain that controls translocation of DNA by recognition of KOPS motifs and interacts with XerD recombinase (By similarity).By similarity

Sequence similaritiesi

Belongs to the FtsK/SpoIIIE/SftA family.Curated
Contains 1 FtsK domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CNU. Bacteria.
COG1674. LUCA.
HOGENOMiHOG000010003.
KOiK03466.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR025199. FtsK_4TM.
IPR002543. FtsK_dom.
IPR018541. Ftsk_gamma.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF13491. FtsK_4TM. 1 hit.
PF09397. Ftsk_gamma. 1 hit.
PF01580. FtsK_SpoIIIE. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00843. Ftsk_gamma. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50901. FTSK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U526-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRMHSPTFD GRHTRFVLTA FFVRQIMALA GFALLATIAL GIAALATWNV
60 70 80 90 100
ADPSLSYATG NQPTNLLGYS GAIFADIVMQ FLGLSAIIAF LPIIAWAIAL
110 120 130 140 150
IAGRKFNRIP ARLVAWVAGA IVCAASLGCF PAPVTWPLPN GIGGVIGDMI
160 170 180 190 200
LRFPALFIGA YPTGTIATIL GVIFAAPAAW LMLFAAGIVG GLDDELEREE
210 220 230 240 250
AVPVATSKAR AAREAEEDDE DDGEGFFANM LAFGAIAHYW YITQARLRRL
260 270 280 290 300
FGLKSKSLHT EFEHPYDFNE YEFGTLNEPS RLKTAINRLD QRSEPSFEER
310 320 330 340 350
AASRRQMSPP SIALDHDNNA DDEPPFDADG RRLPNGILSD DESDDKFTPR
360 370 380 390 400
QAPGRGQPRI TAPSARPKPS ERVAREAQAS FIAADGFQLP TVHLLAEPKN
410 420 430 440 450
VVRDNTLSEE VLEQNARLLE GVLEDFGVKG EIIHVRPGPV VTLYELEPAP
460 470 480 490 500
GIKSSRVIGL ADDIARSMSA IAARVAVVPG RNAIGIELPN QTRETVFLRE
510 520 530 540 550
LVGSRDFENS KAKLAMALGK TIGGEPVIAD LAKMPHLLVA GTTGSGKSVA
560 570 580 590 600
INTMILSLIY RMSPEQCRLI MIDPKMLELS IYDGIPHLLS PVVTDPKKAV
610 620 630 640 650
VALKWTVREM EERYKKMSKI GVRNIDGFNS RVQQALDKGE ILTRTVQTGF
660 670 680 690 700
DRQTGEAMYE TEEFDLKPLP YIVVIIDEMA DLMMVAGKDI EGAVQRLAQM
710 720 730 740 750
ARAAGIHVIM ATQRPSVDVI TGTIKANFPT RISFQVTSKI DSRTILGEQG
760 770 780 790 800
AEQLLGMGDM LYMAGGGRIQ RVHGPFVSDN EVEEIVAYLK TQGSPEYLEA
810 820 830 840 850
ITEEEDEDGA GSGPAGAGNF SDSEDPYDQA VAVVLRDGKA STSYVQRRLG
860 870 880 890
IGYNRAASLI ERMEQEGIIG PANHAGKREI LVPTEADIIE R
Length:891
Mass (Da):96,835
Last modified:April 16, 2014 - v3
Checksum:iE49EF79F664A293C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK88474.2.
PIRiA97690.
AF2915.
RefSeqiNP_355689.2. NC_003062.2.
WP_010972546.1. NC_003062.2.

Genome annotation databases

EnsemblBacteriaiAAK88474; AAK88474; Atu2759.
GeneIDi1134797.
KEGGiatu:Atu2759.
PATRICi20815311. VBIAgrTum91616_2768.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK88474.2.
PIRiA97690.
AF2915.
RefSeqiNP_355689.2. NC_003062.2.
WP_010972546.1. NC_003062.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176299.Atu2759.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK88474; AAK88474; Atu2759.
GeneIDi1134797.
KEGGiatu:Atu2759.
PATRICi20815311. VBIAgrTum91616_2768.

Phylogenomic databases

eggNOGiENOG4105CNU. Bacteria.
COG1674. LUCA.
HOGENOMiHOG000010003.
KOiK03466.

Enzyme and pathway databases

BioCyciAGRO:ATU2759-MONOMER.
RETL1328306-WGS:GSTH-4060-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR025199. FtsK_4TM.
IPR002543. FtsK_dom.
IPR018541. Ftsk_gamma.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF13491. FtsK_4TM. 1 hit.
PF09397. Ftsk_gamma. 1 hit.
PF01580. FtsK_SpoIIIE. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00843. Ftsk_gamma. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50901. FTSK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFTSK_AGRFC
AccessioniPrimary (citable) accession number: Q8U526
Secondary accession number(s): Q8UBU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: April 16, 2014
Last modified: September 7, 2016
This is version 85 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.