Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RNA 2',3'-cyclic phosphodiesterase

Gene

PF0027

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester. In vitro, ligates 5' and 3' half-tRNA molecules with 2',3'-cyclic phosphate and 5'-hydroxyl termini, respectively, to the product containing the 2'-5' phosphodiester linkage. Ligase activity requires GTP, but GTP hydrolysis is not required for the reaction, which is reversible. Ligase activity is weak compared to the phosphodiesterase activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei40Proton donorUniRule annotation1
Active sitei125Proton acceptorUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi6.5.1.B3. 5243.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 2',3'-cyclic phosphodiesteraseUniRule annotation (EC:3.1.4.-UniRule annotation1 Publication)
Short name:
RNA 2',3'-CPDaseUniRule annotation
Gene namesi
Ordered Locus Names:PF0027Imported
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004318031 – 184RNA 2',3'-cyclic phosphodiesteraseAdd BLAST184

Proteomic databases

PRIDEiQ8U4Q3.

Interactioni

Protein-protein interaction databases

STRINGi186497.PF0027.

Structurei

Secondary structure

1184
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi11 – 24Combined sources14
Beta strandi26 – 29Combined sources4
Beta strandi31 – 33Combined sources3
Helixi36 – 38Combined sources3
Beta strandi41 – 48Combined sources8
Helixi53 – 67Combined sources15
Beta strandi72 – 83Combined sources12
Beta strandi86 – 96Combined sources11
Helixi98 – 114Combined sources17
Beta strandi125 – 131Combined sources7
Helixi137 – 146Combined sources10
Turni147 – 149Combined sources3
Beta strandi151 – 156Combined sources6
Beta strandi159 – 168Combined sources10
Beta strandi171 – 180Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FYHNMR-A1-184[»]
ProteinModelPortaliQ8U4Q3.
SMRiQ8U4Q3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U4Q3.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi40 – 43HXTX 1UniRule annotation1 Publication4
Motifi125 – 128HXTX 2UniRule annotation1 Publication4

Sequence similaritiesi

Belongs to the 2H phosphoesterase superfamily. ThpR family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01736. Archaea.
COG1514. LUCA.
HOGENOMiHOG000226389.
KOiK01975.
OMAiIGVFPNP.

Family and domain databases

Gene3Di3.90.1140.10. 1 hit.
HAMAPiMF_01940. RNA_CPDase. 1 hit.
InterProiIPR014051. Phosphoesterase_HXTX.
IPR004175. RNA_CPDase.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PfamiPF02834. LigT_PEase. 2 hits.
[Graphical view]
SUPFAMiSSF55144. SSF55144. 1 hit.
TIGRFAMsiTIGR02258. 2_5_ligase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8U4Q3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAFIAIDVS ESVRDALVRA QDYIGSKEAK IKFVERENFH ITLKFLGEIT
60 70 80 90 100
EEQAEEIKKI LEKIAKKYKK HEVNVRGIGV FPNPNYVRVI WAGVENDEII
110 120 130 140 150
KKIAKEIDDE LAKLGFKKEG NFVAHITLGR VKFVKDKLGL AMKLKELANE
160 170 180
DFGSFIVEAI ELKKSTLTPK GPIYETLARF ELSE
Length:184
Mass (Da):20,995
Last modified:June 1, 2002 - v1
Checksum:i99B45A0210A31E33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL80151.1.
RefSeqiWP_011011139.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80151; AAL80151; PF0027.
GeneIDi1467856.
KEGGipfu:PF0027.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL80151.1.
RefSeqiWP_011011139.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FYHNMR-A1-184[»]
ProteinModelPortaliQ8U4Q3.
SMRiQ8U4Q3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0027.

Proteomic databases

PRIDEiQ8U4Q3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80151; AAL80151; PF0027.
GeneIDi1467856.
KEGGipfu:PF0027.

Phylogenomic databases

eggNOGiarCOG01736. Archaea.
COG1514. LUCA.
HOGENOMiHOG000226389.
KOiK01975.
OMAiIGVFPNP.

Enzyme and pathway databases

BRENDAi6.5.1.B3. 5243.

Miscellaneous databases

EvolutionaryTraceiQ8U4Q3.

Family and domain databases

Gene3Di3.90.1140.10. 1 hit.
HAMAPiMF_01940. RNA_CPDase. 1 hit.
InterProiIPR014051. Phosphoesterase_HXTX.
IPR004175. RNA_CPDase.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PfamiPF02834. LigT_PEase. 2 hits.
[Graphical view]
SUPFAMiSSF55144. SSF55144. 1 hit.
TIGRFAMsiTIGR02258. 2_5_ligase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHPR_PYRFU
AccessioniPrimary (citable) accession number: Q8U4Q3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 4, 2015
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.