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Protein

RNA 2',3'-cyclic phosphodiesterase

Gene

PF0027

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester. In vitro, ligates 5' and 3' half-tRNA molecules with 2',3'-cyclic phosphate and 5'-hydroxyl termini, respectively, to the product containing the 2'-5' phosphodiester linkage. Ligase activity requires GTP, but GTP hydrolysis is not required for the reaction, which is reversible. Ligase activity is weak compared to the phosphodiesterase activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei40Proton donorUniRule annotation1
Active sitei125Proton acceptorUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BRENDAi6.5.1.B3 5243

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 2',3'-cyclic phosphodiesteraseUniRule annotation (EC:3.1.4.-UniRule annotation1 Publication)
Short name:
RNA 2',3'-CPDaseUniRule annotation
Gene namesi
Ordered Locus Names:PF0027Imported
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004318031 – 184RNA 2',3'-cyclic phosphodiesteraseAdd BLAST184

Proteomic databases

PRIDEiQ8U4Q3

Interactioni

Protein-protein interaction databases

STRINGi186497.PF0027

Structurei

Secondary structure

1184
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi11 – 24Combined sources14
Beta strandi26 – 29Combined sources4
Beta strandi31 – 33Combined sources3
Helixi36 – 38Combined sources3
Beta strandi41 – 48Combined sources8
Helixi53 – 67Combined sources15
Beta strandi72 – 83Combined sources12
Beta strandi86 – 96Combined sources11
Helixi98 – 114Combined sources17
Beta strandi125 – 131Combined sources7
Helixi137 – 146Combined sources10
Turni147 – 149Combined sources3
Beta strandi151 – 156Combined sources6
Beta strandi159 – 168Combined sources10
Beta strandi171 – 180Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FYHNMR-A1-184[»]
ProteinModelPortaliQ8U4Q3
SMRiQ8U4Q3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U4Q3

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi40 – 43HXTX 1UniRule annotation1 Publication4
Motifi125 – 128HXTX 2UniRule annotation1 Publication4

Sequence similaritiesi

Belongs to the 2H phosphoesterase superfamily. ThpR family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01736 Archaea
COG1514 LUCA
HOGENOMiHOG000226389
KOiK01975
OMAiLTPRGPI
OrthoDBiPOG093Z0B37

Family and domain databases

HAMAPiMF_01940 RNA_CPDase, 1 hit
InterProiView protein in InterPro
IPR009097 cNuc_Pdiesterase
IPR014051 Phosphoesterase_HXTX
IPR004175 RNA_CPDase
PANTHERiPTHR35561 PTHR35561, 1 hit
PfamiView protein in Pfam
PF02834 LigT_PEase, 2 hits
SUPFAMiSSF55144 SSF55144, 1 hit
TIGRFAMsiTIGR02258 2_5_ligase, 1 hit

Sequencei

Sequence statusi: Complete.

Q8U4Q3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAFIAIDVS ESVRDALVRA QDYIGSKEAK IKFVERENFH ITLKFLGEIT
60 70 80 90 100
EEQAEEIKKI LEKIAKKYKK HEVNVRGIGV FPNPNYVRVI WAGVENDEII
110 120 130 140 150
KKIAKEIDDE LAKLGFKKEG NFVAHITLGR VKFVKDKLGL AMKLKELANE
160 170 180
DFGSFIVEAI ELKKSTLTPK GPIYETLARF ELSE
Length:184
Mass (Da):20,995
Last modified:June 1, 2002 - v1
Checksum:i99B45A0210A31E33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA Translation: AAL80151.1
RefSeqiWP_011011139.1, NC_003413.1

Genome annotation databases

EnsemblBacteriaiAAL80151; AAL80151; PF0027
GeneIDi1467856
KEGGipfu:PF0027
PATRICifig|186497.12.peg.29

Similar proteinsi

Entry informationi

Entry nameiTHPR_PYRFU
AccessioniPrimary (citable) accession number: Q8U4Q3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 4, 2015
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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