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Q8U4M2

- FLPA_PYRFU

UniProt

Q8U4M2 - FLPA_PYRFU

Protein

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase

Gene

flpA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.2 PublicationsUniRule annotation

    GO - Molecular functioni

    1. methyltransferase activity Source: UniProtKB-HAMAP
    2. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. rRNA processing Source: UniProtKB-HAMAP
    2. tRNA processing Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    rRNA processing, tRNA processing

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fibrillarin-like rRNA/tRNA 2'-O-methyltransferaseUniRule annotation (EC:2.1.1.-UniRule annotation)
    Gene namesi
    Name:flpAUniRule annotation
    Ordered Locus Names:PF0059
    OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    Taxonomic identifieri186497 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000001013: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 227227Fibrillarin-like rRNA/tRNA 2'-O-methyltransferasePRO_0000148545Add
    BLAST

    Proteomic databases

    PRIDEiQ8U4M2.

    Interactioni

    Subunit structurei

    Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA. These sRNP particles form homodimers, giving rise to an asymmetric holoenzyme.4 PublicationsUniRule annotation

    Protein-protein interaction databases

    DIPiDIP-60606N.
    STRINGi186497.PF0059.

    Structurei

    Secondary structure

    1
    227
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 64
    Beta strandi12 – 165
    Beta strandi18 – 203
    Beta strandi22 – 276
    Beta strandi29 – 324
    Beta strandi35 – 373
    Beta strandi40 – 434
    Beta strandi46 – 505
    Turni53 – 553
    Helixi57 – 637
    Beta strandi76 – 816
    Helixi87 – 959
    Turni96 – 983
    Beta strandi99 – 1068
    Helixi108 – 11811
    Beta strandi124 – 1285
    Beta strandi131 – 1333
    Helixi134 – 1407
    Beta strandi144 – 1496
    Helixi156 – 16712
    Beta strandi168 – 17912
    Helixi180 – 1834
    Beta strandi185 – 1873
    Helixi189 – 20113
    Beta strandi204 – 2118
    Turni213 – 2153
    Beta strandi216 – 22510

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PRYX-ray1.97A1-227[»]
    2NNWX-ray2.70B/D1-227[»]
    3NMUX-ray2.73F/J1-227[»]
    3NVKX-ray3.21I/J1-227[»]
    3NVMX-ray3.41B1-227[»]
    4BY9NMR-E/H/K/N1-227[»]
    ProteinModelPortaliQ8U4M2.
    SMRiQ8U4M2. Positions 1-227.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8U4M2.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni86 – 872S-adenosyl-L-methionine bindingUniRule annotation
    Regioni105 – 1062S-adenosyl-L-methionine binding
    Regioni130 – 1312S-adenosyl-L-methionine binding
    Regioni150 – 1534S-adenosyl-L-methionine binding

    Sequence similaritiesi

    Belongs to the methyltransferase superfamily. Fibrillarin family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1889.
    HOGENOMiHOG000106741.
    KOiK04795.
    OMAiWNPNKSK.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_00351. RNA_methyltransf_FlpA.
    InterProiIPR000692. Fibrillarin.
    IPR020813. Fibrillarin_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01269. Fibrillarin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006540. Nop17p. 1 hit.
    PRINTSiPR00052. FIBRILLARIN.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00566. FIBRILLARIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8U4M2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVEVKKHKFP GVYVVIDDDG SEKIATKNLV PGQRVYGERV IKWEGEEYRI    50
    WNPHRSKLGA AIVNGLKNFP IKPGKSVLYL GIASGTTASH VSDIVGWEGK 100
    IYGIEFSPRV LRELVPIVEE RRNIIPILGD ATKPEEYRAL VTKVDVIFED 150
    VAQPTQAKIL IDNAKAYLKR GGYGMIAVKS RSIDVTKEPE QVFKEVEREL 200
    SEYFEVIERL NLEPYEKDHA LFVVRKP 227
    Length:227
    Mass (Da):25,762
    Last modified:June 1, 2002 - v1
    Checksum:iA2190B2983FD6EF3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009950 Genomic DNA. Translation: AAL80183.1.
    RefSeqiNP_577788.1. NC_003413.1.
    WP_011011171.1. NC_003413.1.

    Genome annotation databases

    EnsemblBacteriaiAAL80183; AAL80183; PF0059.
    GeneIDi1467888.
    KEGGipfu:PF0059.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009950 Genomic DNA. Translation: AAL80183.1 .
    RefSeqi NP_577788.1. NC_003413.1.
    WP_011011171.1. NC_003413.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1PRY X-ray 1.97 A 1-227 [» ]
    2NNW X-ray 2.70 B/D 1-227 [» ]
    3NMU X-ray 2.73 F/J 1-227 [» ]
    3NVK X-ray 3.21 I/J 1-227 [» ]
    3NVM X-ray 3.41 B 1-227 [» ]
    4BY9 NMR - E/H/K/N 1-227 [» ]
    ProteinModelPortali Q8U4M2.
    SMRi Q8U4M2. Positions 1-227.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60606N.
    STRINGi 186497.PF0059.

    Proteomic databases

    PRIDEi Q8U4M2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL80183 ; AAL80183 ; PF0059 .
    GeneIDi 1467888.
    KEGGi pfu:PF0059.

    Phylogenomic databases

    eggNOGi COG1889.
    HOGENOMi HOG000106741.
    KOi K04795.
    OMAi WNPNKSK.

    Miscellaneous databases

    EvolutionaryTracei Q8U4M2.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_00351. RNA_methyltransf_FlpA.
    InterProi IPR000692. Fibrillarin.
    IPR020813. Fibrillarin_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01269. Fibrillarin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006540. Nop17p. 1 hit.
    PRINTSi PR00052. FIBRILLARIN.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00566. FIBRILLARIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
      Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
      Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
    2. "Structure determination of fibrillarin from the hyperthermophilic archaeon Pyrococcus furiosus."
      Deng L., Starostina N.G., Liu Z.J., Rose J.P., Terns R.M., Terns M.P., Wang B.C.
      Biochem. Biophys. Res. Commun. 315:726-732(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.97 ANGSTROMS), SUBUNIT.
    3. "Alternative conformations of the archaeal Nop56/58-fibrillarin complex imply flexibility in box C/D RNPs."
      Oruganti S., Zhang Y., Li H., Robinson H., Terns M.P., Terns R.M., Yang W., Li H.
      J. Mol. Biol. 371:1141-1150(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH NOP5, SUBUNIT.
    4. "Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle."
      Xue S., Wang R., Yang F., Terns R.M., Terns M.P., Zhang X., Maxwell E.S., Li H.
      Mol. Cell 39:939-949(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.73 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-METHIONINE; RPL7AE; NOP5 AND RNA SUBSTRATE, SUBUNIT, FUNCTION.
    5. "The structure of the box C/D enzyme reveals regulation of RNA methylation."
      Lapinaite A., Simon B., Skjaerven L., Rakwalska-Bange M., Gabel F., Carlomagno T.
      Nature 502:519-523(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR IN COMPLEX WITH RPL7AE; NOP5 AND RNA SUBSTRATE, FUNCTION, SUBUNIT.

    Entry informationi

    Entry nameiFLPA_PYRFU
    AccessioniPrimary (citable) accession number: Q8U4M2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 26, 2002
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3