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Q8U4M2 (FLPA_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase

EC=2.1.1.-
Gene names
Name:flpA
Ordered Locus Names:PF0059
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Ref.4 Ref.5

Subunit structure

Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA. These sRNP particles form homodimers, giving rise to an asymmetric holoenzyme. Ref.2 Ref.3 Ref.4 Ref.5

Sequence similarities

Belongs to the methyltransferase superfamily. Fibrillarin family.

Ontologies

Keywords
   Biological processrRNA processing
tRNA processing
   LigandRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processrRNA processing

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA processing

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 227227Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase HAMAP-Rule MF_00351
PRO_0000148545

Regions

Region86 – 872S-adenosyl-L-methionine binding By similarity
Region105 – 1062S-adenosyl-L-methionine binding HAMAP-Rule MF_00351
Region130 – 1312S-adenosyl-L-methionine binding HAMAP-Rule MF_00351
Region150 – 1534S-adenosyl-L-methionine binding HAMAP-Rule MF_00351

Secondary structure

................................................. 227
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8U4M2 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: A2190B2983FD6EF3

FASTA22725,762
        10         20         30         40         50         60 
MVEVKKHKFP GVYVVIDDDG SEKIATKNLV PGQRVYGERV IKWEGEEYRI WNPHRSKLGA 

        70         80         90        100        110        120 
AIVNGLKNFP IKPGKSVLYL GIASGTTASH VSDIVGWEGK IYGIEFSPRV LRELVPIVEE 

       130        140        150        160        170        180 
RRNIIPILGD ATKPEEYRAL VTKVDVIFED VAQPTQAKIL IDNAKAYLKR GGYGMIAVKS 

       190        200        210        220 
RSIDVTKEPE QVFKEVEREL SEYFEVIERL NLEPYEKDHA LFVVRKP 

« Hide

References

« Hide 'large scale' references
[1]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]"Structure determination of fibrillarin from the hyperthermophilic archaeon Pyrococcus furiosus."
Deng L., Starostina N.G., Liu Z.J., Rose J.P., Terns R.M., Terns M.P., Wang B.C.
Biochem. Biophys. Res. Commun. 315:726-732(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.97 ANGSTROMS), SUBUNIT.
[3]"Alternative conformations of the archaeal Nop56/58-fibrillarin complex imply flexibility in box C/D RNPs."
Oruganti S., Zhang Y., Li H., Robinson H., Terns M.P., Terns R.M., Yang W., Li H.
J. Mol. Biol. 371:1141-1150(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH NOP5, SUBUNIT.
[4]"Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle."
Xue S., Wang R., Yang F., Terns R.M., Terns M.P., Zhang X., Maxwell E.S., Li H.
Mol. Cell 39:939-949(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.73 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-METHIONINE; RPL7AE; NOP5 AND RNA SUBSTRATE, SUBUNIT, FUNCTION.
[5]"The structure of the box C/D enzyme reveals regulation of RNA methylation."
Lapinaite A., Simon B., Skjaerven L., Rakwalska-Bange M., Gabel F., Carlomagno T.
Nature 502:519-523(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR IN COMPLEX WITH RPL7AE; NOP5 AND RNA SUBSTRATE, FUNCTION, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009950 Genomic DNA. Translation: AAL80183.1.
RefSeqNP_577788.1. NC_003413.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1PRYX-ray1.97A1-227[»]
2NNWX-ray2.70B/D1-227[»]
3NMUX-ray2.73F/J1-227[»]
3NVKX-ray3.21I/J1-227[»]
3NVMX-ray3.41B1-227[»]
4BY9NMR-E/H/K/N1-227[»]
ProteinModelPortalQ8U4M2.
SMRQ8U4M2. Positions 1-227.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60606N.
STRING186497.PF0059.

Proteomic databases

PRIDEQ8U4M2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL80183; AAL80183; PF0059.
GeneID1467888.
KEGGpfu:PF0059.

Phylogenomic databases

eggNOGCOG1889.
HOGENOMHOG000106741.
KOK04795.
OMAWNPNKSK.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_00351. RNA_methyltransf_FlpA.
InterProIPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF01269. Fibrillarin. 1 hit.
[Graphical view]
PIRSFPIRSF006540. Nop17p. 1 hit.
PRINTSPR00052. FIBRILLARIN.
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS00566. FIBRILLARIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8U4M2.

Entry information

Entry nameFLPA_PYRFU
AccessionPrimary (citable) accession number: Q8U4M2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references