Q8U4M2 (FLPA_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
EC=2.1.1.-

Gene names

Name:	flpA
Ordered Locus Names:	PF0059

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA By similarity. HAMAP MF_00351
Subunit structure	Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, flpA and nop5, plus a guide RNA By similarity. Ref.2
Sequence similarities	Belongs to the methyltransferase superfamily. Fibrillarin family.

Ontologies

Keywords
Biological process	rRNA processing tRNA processing
Ligand	RNA-binding
Molecular function	Methyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 227

227

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase HAMAP MF_00351

PRO_0000148545

Regions

Region

86 – 87

2

S-adenosyl-L-methionine binding By similarity

Region

105 – 106

2

S-adenosyl-L-methionine binding By similarity

Region

130 – 131

2

S-adenosyl-L-methionine binding By similarity

Sites

Binding site

57

1

S-adenosyl-L-methionine By similarity

Binding site

150

1

S-adenosyl-L-methionine By similarity

Secondary structure

1

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227

Helix Strand Turn

Details...

Beta strand

3 – 6

4

Beta strand

12 – 16

5

Beta strand

22 – 27

6

Beta strand

40 – 43

4

Beta strand

46 – 50

5

Turn

53 – 55

3

Helix

57 – 63

7

Beta strand

76 – 81

6

Helix

87 – 95

9

Beta strand

99 – 106

8

Helix

108 – 118

11

Beta strand

124 – 128

5

Helix

134 – 140

7

Beta strand

144 – 149

6

Helix

156 – 167

12

Beta strand

168 – 179

12

Helix

180 – 183

4

Beta strand

185 – 187

3

Helix

189 – 201

13

Beta strand

204 – 211

8

Turn

213 – 215

3

Beta strand

216 – 225

10

Sequences

	Sequence		Length	Mass (Da)
	Q8U4M2 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: A2190B2983FD6EF3	FASTA	227	25,762
	10 20 30 40 50 60 MVEVKKHKFP GVYVVIDDDG SEKIATKNLV PGQRVYGERV IKWEGEEYRI WNPHRSKLGA 70 80 90 100 110 120 AIVNGLKNFP IKPGKSVLYL GIASGTTASH VSDIVGWEGK IYGIEFSPRV LRELVPIVEE 130 140 150 160 170 180 RRNIIPILGD ATKPEEYRAL VTKVDVIFED VAQPTQAKIL IDNAKAYLKR GGYGMIAVKS 190 200 210 220 RSIDVTKEPE QVFKEVEREL SEYFEVIERL NLEPYEKDHA LFVVRKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]	"Structure determination of fibrillarin from the hyperthermophilic archaeon Pyrococcus furiosus." Deng L., Starostina N.G., Liu Z.J., Rose J.P., Terns R.M., Terns M.P., Wang B.C. Biochem. Biophys. Res. Commun. 315:726-732(2004) [PubMed: 14975761] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.97 ANGSTROMS), SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE009950 Genomic DNA. Translation: AAL80183.1.

RefSeq

NP_577788.1. NC_003413.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PRY	X-ray	1.97	A	1-227	[»]
2NNW	X-ray	2.70	B/D	1-227	[»]
3NMU	X-ray	2.73	F/J	1-227	[»]
3NVK	X-ray	3.21	I/J	1-227	[»]
3NVM	X-ray	3.41	B	1-227	[»]

ProteinModelPortal

Q8U4M2.

SMR

Q8U4M2. Positions 1-227.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBPYRT00000005196; EBPYRP00000005057; EBPYRG00000005195.

GeneID

1467888.

GenomeReviews

Gene locus PF0059 in contig AE009950_GR.

KEGG

pfu:PF0059.

NMPDR

fig|186497.1.peg.59.

Phylogenomic databases

GeneTree

EBGT00050000023119.

HOGENOM

HBG297404.

OMA

YERDHCI.

PhylomeDB

Q8U4M2.

ProtClustDB

PRK04266.

Family and domain databases

HAMAP

MF_00351. RNA_methyltransf_FlpA.
[Tree]

InterPro

IPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
[Graphical view]

KO

K04795.

PANTHER

PTHR10335. Fibrillarin. 1 hit.

Pfam

PF01269. Fibrillarin. 1 hit.
[Graphical view]

PIRSF

PIRSF006540. Nop17p. 1 hit.

PRINTS

PR00052. FIBRILLARIN.

PROSITE

PS00566. FIBRILLARIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

FLPA_PYRFU

Accession

Primary (citable) accession number: Q8U4M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program