Q8U4H6 (PRNK_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polyribonucleotide 5'-hydroxyl-kinase PF0112
EC=2.7.1.78

Alternative name(s):
Polynucleotide kinase PF0112

Gene names

Ordered Locus Names:

PF0112

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of both single-stranded RNA (ssRNA) and single-stranded DNA (ssDNA). Exhibits a strong preference for ssRNA By similarity.
Catalytic activity	ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA. ATP + 5'-dephospho-RNA = ADP + 5'-phospho-RNA.
Cofactor	Divalent cation By similarity.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Polyribonucleotide 5'-hydroxyl-kinase PF0112		PRO_0000376016
Regions
	Nucleotide binding	36 – 43	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8U4H6 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: EF59DC8A90A040A6	FASTA	354	40,054
	10 20 30 40 50 60 MEVNKASLTY DVPEDREYAT KRILSLKRPS KIMVIGDVDT GKTTLIVYLA NELISRGFKV 70 80 90 100 110 120 AIVDADVGQK GILPPATISL ALADMKFSSL SELKPLIHYF VGSITPSQFF GEMIVGTMRL 130 140 150 160 170 180 SEIGKKFADY VLIDTTGMIY GSGVELKRLK IEAVKPDLIL ALEKKEELNP IVSGFEDKTI 190 200 210 220 230 240 KLKVSENARS YSRSERRQIR QEKWRKYFEN AKIVSFSLEN VVVTGTSLFQ GSDIREEEKS 250 260 270 280 290 300 LLERLFKWVI LHGRRIGDKY FVVKADVAEV PRVVDKNVVR YFDFEKLSNL LVGLLNEEGL 310 320 330 340 350 CLGVGIIKGI NFGEKRIDIL TPVSEIENVR EIRFGRIRVR EDGEELGILD REAL

References

[1]

"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009950 Genomic DNA. Translation: AAL80236.1.
RefSeq	NP_577841.1. NC_003413.1.
3D structure databases
ProteinModelPortal	Q8U4H6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000005826; EBPYRP00000005687; EBPYRG00000005825.
GeneID	1467941.
GenomeReviews	Gene locus PF0112 in contig AE009950_GR.
KEGG	pfu:PF0112.
NMPDR	fig|186497.1.peg.112.
Phylogenomic databases
GeneTree	EBGT00050000023291.
HOGENOM	HBG538965.
OMA	NDLGPPT.
ProtClustDB	CLSK544691.
Family and domain databases
InterPro	IPR010655. Pre-mRNA_cleavage_cplxII_Clp1. [Graphical view]
KO	K06947.
Pfam	PF06807. Clp1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRNK_PYRFU

Accession

Primary (citable) accession number: Q8U4H6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program