Reviewed, UniProtKB/Swiss-Prot Q8U4H6 (PRNK_PYRFU)

Names and origin

Protein names

Recommended name:
    Polyribonucleotide 5'-hydroxyl-kinase PF0112
    EC=2.7.1.78
Alternative name(s):
    Polynucleotide kinase PF0112

Gene names

Ordered Locus Names:

PF0112

Organism

Pyrococcus furiosus [Complete proteome] [HAMAP]

Taxonomic identifier

2261 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of both single-stranded RNA (ssRNA) and single-stranded DNA (ssDNA). Exhibits a strong preference for ssRNA By similarity.
Catalytic activity	ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA. ATP + 5'-dephospho-RNA = ADP + 5'-phospho-RNA.
Cofactor	Divalent cation By similarity.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity Inferred from electronic annotation. Source: EC GTP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Polyribonucleotide 5'-hydroxyl-kinase PF0112		PRO_0000376016
Regions
	Nucleotide binding	36 – 43	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8U4H6-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: EF59DC8A90A040A6	FASTA	354	40,054
	10 20 30 40 50 60 MEVNKASLTY DVPEDREYAT KRILSLKRPS KIMVIGDVDT GKTTLIVYLA NELISRGFKV 70 80 90 100 110 120 AIVDADVGQK GILPPATISL ALADMKFSSL SELKPLIHYF VGSITPSQFF GEMIVGTMRL 130 140 150 160 170 180 SEIGKKFADY VLIDTTGMIY GSGVELKRLK IEAVKPDLIL ALEKKEELNP IVSGFEDKTI 190 200 210 220 230 240 KLKVSENARS YSRSERRQIR QEKWRKYFEN AKIVSFSLEN VVVTGTSLFQ GSDIREEEKS 250 260 270 280 290 300 LLERLFKWVI LHGRRIGDKY FVVKADVAEV PRVVDKNVVR YFDFEKLSNL LVGLLNEEGL 310 320 330 340 350 CLGVGIIKGI NFGEKRIDIL TPVSEIENVR EIRFGRIRVR EDGEELGILD REAL

References

[1]	"The complete sequence of the Pyrococcus furiosus genome." Weiss R.B., Dunn D.M., Robb F.T., Brown J.R. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009950 Genomic DNA. Translation: AAL80236.1.
RefSeq	NP_577841.1.
3D structure databases
SMR	Q8U4H6. Positions 29-325.
ModBase	Search...
Genome annotation databases
GeneID	1467941.
GenomeReviews	Gene locus PF0112 in contig AE009950_GR.
KEGG	pfu:PF0112.
NMPDR	fig|186497.1.peg.112.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG538965.
OMA	NDLGPPT.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

PRNK_PYRFU

Accession

Primary (citable) accession number: Q8U4H6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	June 1, 2002
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)