Q8U4E6 (ASGX_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative L-asparaginase
EC=3.5.1.1

Alternative name(s):
L-asparagine amidohydrolase

Cleaved into the following 2 chains:

1. Putative L-asparaginase subunit alpha
2. Putative L-asparaginase subunit beta

Gene names

Ordered Locus Names:

PF0142

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-asparagine + H2O = L-aspartate + NH3.
Post-translational modification	Autocleaved. Generates the alpha and beta subunits. The N-terminal residue of the beta subunit is thought to be responsible for the nucleophile hydrolase activity Potential.
Sequence similarities	Belongs to the Ntn-hydrolase family.

Ontologies

Keywords
Molecular function	Hydrolase Protease
PTM	Autocatalytic cleavage
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	asparaginase activity Inferred from electronic annotation. Source: EC peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 175	175	Putative L-asparaginase subunit alpha		PRO_0000184580
	Chain	176 – 306	131	Putative L-asparaginase subunit beta		PRO_0000329017
Sites
	Active site	176	1	Nucleophile By similarity
	Site	175 – 176	2	Cleavage; by autolysis Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8U4E6 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 68410D8FE6DE9EE2	FASTA	306	32,855
	10 20 30 40 50 60 MVAIIVHGGA GTIRKEERIP KIIEGVREAV LTGWRELKKG SALDAVEEAV KVLEDNPLFN 70 80 90 100 110 120 AGTGSVLTLD GKVEMDAAIM RGKTLDAGAV AGIWGVKNPI SVARKVMEKT DHVLLIGEGA 130 140 150 160 170 180 VKFARLMGFP EYDPTTEERR KQWEELRKKL LETGEIRHWK KLSELIKEYP EVLRSTVGAV 190 200 210 220 230 240 AFDGEEIVAG TSTGGVFLKM FGRVGDTPII GAGTYANEVA GASCTGLGEV AIKLSLAKTA 250 260 270 280 290 300 TDFVRLGLDA QAASEAAIRL ATKYFGPDTM GIIMVDSNGN VGFAKNTKHM SYAFMKEGMK EPEAGV

References

[1]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009950 Genomic DNA. Translation: AAL80266.1.
RefSeq	NP_577871.1. NC_003413.1.
3D structure databases
ProteinModelPortal	Q8U4E6.
ModBase	Search...
Protein family/group databases
MEROPS	T02.002.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000004210; EBPYRP00000004071; EBPYRG00000004210.
GeneID	1467974.
GenomeReviews	Gene locus PF0142 in contig AE009950_GR.
KEGG	pfu:PF0142.
NMPDR	fig|186497.1.peg.145.
Phylogenomic databases
GeneTree	EBGT00050000022719.
HOGENOM	HBG735787.
OMA	ATGHGEF.
PhylomeDB	Q8U4E6.
ProtClustDB	CLSK253204.
Family and domain databases
InterPro	IPR000246. Peptidase_T2. [Graphical view]
KO	K13051.
PANTHER	PTHR10188. Peptidase_T2. 1 hit.
Pfam	PF01112. Asparaginase_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASGX_PYRFU

Accession

Primary (citable) accession number: Q8U4E6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program