Reviewed, UniProtKB/Swiss-Prot Q8U4E6 (ASGX_PYRFU)

Names and origin

Protein names

Recommended name:
    Putative L-asparaginase
    EC=3.5.1.1
Alternative name(s):
    L-asparagine amidohydrolase
Cleaved into the following 2 chains:
    1- Recommended name:
            Putative L-asparaginase subunit alpha
    2- Recommended name:
            Putative L-asparaginase subunit beta

Gene names

Ordered Locus Names:

PF0142

Organism

Pyrococcus furiosus [Complete proteome] [HAMAP]

Taxonomic identifier

2261 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-asparagine + H2O = L-aspartate + NH3.
Post-translational modification	Autocleaved. Generates the alpha and beta subunits. The N-terminal residue of the beta subunit is thought to be responsible for the nucleophile hydrolase activity Potential.
Sequence similarities	Belongs to the Ntn-hydrolase family.

Ontologies

Keywords
Molecular function	Hydrolase Protease
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	asparaginase activity Inferred from electronic annotation. Source: EC peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 175	175	Putative L-asparaginase subunit alpha		PRO_0000184580
	Chain	176 – 306	131	Putative L-asparaginase subunit beta		PRO_0000329017
Sites
	Active site	176	1	Nucleophile By similarity
	Site	175 – 176	2	Cleavage; by autolysis Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8U4E6-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 68410D8FE6DE9EE2	FASTA	306	32,855
	10 20 30 40 50 60 MVAIIVHGGA GTIRKEERIP KIIEGVREAV LTGWRELKKG SALDAVEEAV KVLEDNPLFN 70 80 90 100 110 120 AGTGSVLTLD GKVEMDAAIM RGKTLDAGAV AGIWGVKNPI SVARKVMEKT DHVLLIGEGA 130 140 150 160 170 180 VKFARLMGFP EYDPTTEERR KQWEELRKKL LETGEIRHWK KLSELIKEYP EVLRSTVGAV 190 200 210 220 230 240 AFDGEEIVAG TSTGGVFLKM FGRVGDTPII GAGTYANEVA GASCTGLGEV AIKLSLAKTA 250 260 270 280 290 300 TDFVRLGLDA QAASEAAIRL ATKYFGPDTM GIIMVDSNGN VGFAKNTKHM SYAFMKEGMK EPEAGV

References

[1]	"The complete sequence of the Pyrococcus furiosus genome." Weiss R.B., Dunn D.M., Robb F.T., Brown J.R. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009950 Genomic DNA. Translation: AAL80266.1.
RefSeq	NP_577871.1.
3D structure databases
HSSP	HSSP built from PDB template 9GAF based on UniProtKB Q47898.
ModBase	Search...
Protein family/group databases
MEROPS	T02.002.
Genome annotation databases
GeneID	1467974.
GenomeReviews	Gene locus PF0142 in contig AE009950_GR.
KEGG	pfu:PF0142.
NMPDR	fig|186497.1.peg.145.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8U4E6.
OMA	MVAIIVH.
Enzyme and pathway databases
BRENDA	3.5.1.1. 321.
Family and domain databases
InterPro	IPR000246. Peptidase_T2. [Graphical view]
PANTHER	PTHR10188. Peptidase_T2. 1 hit.
Pfam	PF01112. Asparaginase_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASGX_PYRFU

Accession

Primary (citable) accession number: Q8U4E6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)