Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q8U4D4 (Q8U4D4_PYRFU) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485
Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485
Gene names
Ordered Locus Names:PF0154
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3

Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site121For GATase activity By similarity PIRSR PIRSR000485-1
Metal binding2391Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding3831Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4321Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4351Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

Sequence LengthMass (Da)Tools
Q8U4D4 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 7D27AC751C1065B9

FASTA44950,172
        10         20         30         40         50         60 
MRGESIVIRE KCGIFAAYAD NAPEKAYYSL LSLQHRGQEG AGITFWENGI KTIKGIGLVP 

        70         80         90        100        110        120 
EVFKGGIESK SSLAIGHVRY STFGSLTEVQ PIEVKCCNYK LAIAHNGTLT NYLPLREKYE 

       130        140        150        160        170        180 
SEGFKFNSTI DTELIGVAFL KHYKELGDEF KAMEEVFNEV RGAYSVAILF DGKIIVARDP 

       190        200        210        220        230        240 
AGFRPLAYGE GDGFYFASEN SGLRMFTNKI RDVFPGEVFV VEEGEIYSKV LKKLPHSHCV 

       250        260        270        280        290        300 
FEYIYFARPD SNIEGVSVYL ARYRMGVELA RESPAPGDVV IAVPDSGRTA ALGFAHESGI 

       310        320        330        340        350        360 
PYMEGLIKNR YIGRTFIMPS GRELKVKLKL SPVREVIEGK RVVLVDDSIV RGTTIKRIVK 

       370        380        390        400        410        420 
MIRDAGAEEV HVRISSPPIR YPCYMGIDIP TRHELIAAWR TVEDIRREIG ADSLAYLSIE 

       430        440 
GLKRAVGIED LCMACLTGHY PEWAFDFEL

« Hide

References

[1]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE009950 Genomic DNA. Translation: AAL80278.1.
RefSeqNP_577883.1. NC_003413.1.

3D structure databases

HSSPHSSP built from PDB template 1AO0 based on UniProtKB P00497.
ProteinModelPortalQ8U4D4.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBPYRT00000004906; EBPYRP00000004767; EBPYRG00000004905.
GeneID1467986.
GenomeReviewsGene locus PF0154 in contig AE009950_GR.
KEGGpfu:PF0154.
NMPDRfig|186497.1.peg.157.

Phylogenomic databases

GeneTreeEBGT00050000022516.
HOGENOMHBG392416.
OMADIAQMIC.
PhylomeDBQ8U4D4.
ProtClustDBPRK08341.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR000583. GATase_2.
IPR017932. GATase_II.
IPR000836. PRibTrfase.
[Graphical view]
KOK00764.
PANTHERPTHR11907. Amd_phspho_trans. 1 hit.
PfamPF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. PurF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ8U4D4_PYRFU
AccessionPrimary (citable) accession number: Q8U4D4
Entry history
Integrated into UniProtKB/TrEMBL: June 1, 2002
Last sequence update: June 1, 2002
Last modified: January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info