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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

glmS

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.UniRule annotation

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile; for GATase activityUniRule annotation
Active sitei593 – 5931For Fru-6P isomerization activityUniRule annotation

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. glutamine metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Protein family/group databases

MEROPSiC44.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]UniRule annotation (EC:2.6.1.16UniRule annotation)
Alternative name(s):
D-fructose-6-phosphate amidotransferaseUniRule annotation
GFATUniRule annotation
Glucosamine-6-phosphate synthaseUniRule annotation
Hexosephosphate aminotransferaseUniRule annotation
L-glutamine--D-fructose-6-phosphate amidotransferaseUniRule annotation
Gene namesi
Name:glmSUniRule annotation
Ordered Locus Names:PF0157
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedUniRule annotation
Chaini2 – 598597Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135430Add
BLAST

Proteomic databases

PRIDEiQ8U4D1.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi186497.PF0157.

Structurei

3D structure databases

ProteinModelPortaliQ8U4D1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 219218Glutamine amidotransferase type-2UniRule annotationAdd
BLAST
Domaini280 – 420141SIS 1UniRule annotationAdd
BLAST
Domaini449 – 588140SIS 2UniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation
Contains 2 SIS domains.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258898.
KOiK00820.
OMAiDCLEVFK.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8U4D1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGIIGYIGP RRASPILVEG LKRLEYRGYD SAGIAVLNEG RIEIRKGAGK
60 70 80 90 100
IDELVGKLKF QEIPGNIGIG HTRWATHGVP NDINAHPHTD CTGKIVVVHN
110 120 130 140 150
GIVENFHELK EELLKKGHVF RSDTDTELIA HLIEENLRII KNFEDAFRMA
160 170 180 190 200
LLRLKGSYAL VVLFADDPER LYIARKDSPL IIGIGNGEMF VASDIPAFLA
210 220 230 240 250
YTNKAVFLDD GEYGIVSRYE FIVKDIVTGF PKIKDVVTIS WTLEMAEKGG
260 270 280 290 300
FDHFMLKEIY EQPRAIKDAI YGNIEAIKEV AEVVSNYDKI IMIGMGTSYH
310 320 330 340 350
AALVGKYLFQ RFTKTPVIVE EASEFRYEYE DIIDKDTLVV AITQSGETAD
360 370 380 390 400
TVAGIKLAKN KGAKVLGIVN VVGSLATRIA DITLYTHAGP EIGVAATKTY
410 420 430 440 450
TTQLMVLTLL AMELGKKIGT DVSEIENILP RLPEIIDSAL GLNEKIKGIA
460 470 480 490 500
KNILPKHDLF YIGRGINVPT ALEGALKIKE IAYVHAEGLS AGELKHGPLA
510 520 530 540 550
LIEEGVPVIS IAPSGKTFEK MLSNIEEAKA RGGYIISLGD DIRLSEISDE
560 570 580 590
WIRLPKLPEE ITPIVYIVPL QLLAYHLAVL RGNNPDRPRN LAKSVTVE
Length:598
Mass (Da):65,884
Last modified:January 23, 2007 - v3
Checksum:i7896717DB2143121
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL80281.1.
RefSeqiNP_577886.1. NC_003413.1.
WP_011011270.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80281; AAL80281; PF0157.
GeneIDi1467989.
KEGGipfu:PF0157.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL80281.1.
RefSeqiNP_577886.1. NC_003413.1.
WP_011011270.1. NC_003413.1.

3D structure databases

ProteinModelPortaliQ8U4D1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0157.

Protein family/group databases

MEROPSiC44.971.

Proteomic databases

PRIDEiQ8U4D1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80281; AAL80281; PF0157.
GeneIDi1467989.
KEGGipfu:PF0157.

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258898.
KOiK00820.
OMAiDCLEVFK.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Entry informationi

Entry nameiGLMS_PYRFU
AccessioniPrimary (citable) accession number: Q8U4D1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: January 23, 2007
Last modified: February 4, 2015
This is version 77 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.