Reviewed, UniProtKB/Swiss-Prot Q8U4A6 (VATA_PYRFU)

Names and origin

Protein names

Recommended name:
    V-type ATP synthase alpha chain
    EC=3.6.3.14
Alternative name(s):
    V-ATPase subunit A
Cleaved into the following chain:
    1- Recommended name:
            Endonuclease PI-Pfu2
              EC=3.1.-.-
        Alternative name(s):
            Pfu atpA intein
            Pfu VMA intein

Gene names

Name:	atpA
Ordered Locus Names:	PF0182

Organism

Pyrococcus furiosus [Complete proteome] [HAMAP]

Taxonomic identifier

2261 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	1013 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit. HAMAP MF_00309
Catalytic activity	ATP + H2O + H+(In) = ADP + phosphate + H+(Out). HAMAP MF_00309
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the VDE intervening region (intein) followed by peptide ligation Potential.
Miscellaneous	The intein interrupts the ATP-binding site. HAMAP MF_00309
Sequence similarities	Belongs to the ATPase alpha/beta chains family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	ATP synthesis Hydrogen ion transport Intron homing Ion transport Transport
Ligand	ATP-binding Nucleotide-binding
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane ATP synthesis coupled proton transport Inferred from electronic annotation. Source: HAMAP
Cellular component	proton-transporting two-sector ATPase complex, catalytic domain Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW hydrogen ion transporting ATP synthase activity, rotational mechanism Inferred from electronic annotation. Source: HAMAP proton-transporting ATPase activity, rotational mechanism Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	V-type ATP synthase alpha chain, 1st part Potential		PRO_0000002464
	Chain	241 – 665	425	Endonuclease PI-Pfu2 Potential		PRO_0000002465
	Chain	666 – 1013	348	V-type ATP synthase alpha chain, 2nd part Potential		PRO_0000002466
Regions
	Domain	392 – 525	134	DOD-type homing endonuclease

Sequences

	Sequence		Length	Mass (Da)
	Q8U4A6-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: CAC1CB86566C81CD	FASTA	1,013	113,452
	10 20 30 40 50 60 MPAKGRIIRV TGPLVIADGM KGAKMYEVVR VGELGLIGEI IRLEGDKAVI QVYEETAGLK 70 80 90 100 110 120 PGEPVEGTGS SLSVELGPGL LTSIYDGIQR PLEVLREKSG HFIARGISAP ALPRDKKWHF 130 140 150 160 170 180 TPKVKVGDKV VGGDIIGEVP ETSIIVHKIM VPPGIEGEIV EIADEGEYTI EEVIAKVKTP 190 200 210 220 230 240 SGEIKELKMY QRWPVRVKRP YKEKLPPEVP LVTGQRVIDT FFPQAKGGTA AIPGPFGSGK 250 260 270 280 290 300 CVDGDTLILT KEFGLIKIKD LYEKLDGKGR KTVEGNEEWT ELEEPITVYG YKNGKIVEIK 310 320 330 340 350 360 ATHVYKGASS GMIEIKTRTG RKIKVTPIHK LFTGRVTKDG LVLEEVMAMH IKPGDRIAVV 370 380 390 400 410 420 KKIDGGEYVK LDTSSVTKIK VPEVLNEELA EFLGYVIGDG TLKPRTVAIY NNDESLLKRA 430 440 450 460 470 480 NFLAMKLFGV SGKIVQERTV KALLIHSKYL VDFLKKLGIP GNKKARTWKV PKELLLSPPS 490 500 510 520 530 540 VVKAFINAYI ACDGYYNKEK GEIEIVTASE EGAYGLTYLL AKLGIYATIR RKTINGREYY 550 560 570 580 590 600 RVVISGKANL EKLGVKREAR GYTSIDVVPV DVESIYEALG RPYSELKKEG IEIHNYLSGE 610 620 630 640 650 660 NMSYETFRKF AKVVGLEEIA ENHLQHILFD EVVEVNYISE PQEVYDITTE THNFVGGNMP 670 680 690 700 710 720 TLLHNTVTQH QLAKWSDAQV VVYIGCGERG NEMTDVLEEF PKLKDPNTGK PLMERTVLIA 730 740 750 760 770 780 NTSNMPVAAR EASIYTGITI AEYFRDMGYD VALMADSTSR WAEALREISG RLEEMPGEEG 790 800 810 820 830 840 YPAYLASRLA EFYERAGRVV TLGSDYRVGS VSVIGAVSPP GGDFSEPVVQ NTLRVVKVFW 850 860 870 880 890 900 ALDADLARRR HFPAINWLTS YSLYVDAVQD WWHKNVDPEW RRMRDKAMEL LQKEAELQEI 910 920 930 940 950 960 VRIVGPDALP ERERAILLVA RMLREDYLQQ DAFDEVDTYC PPQKQVTMMR VLMTFYERTM 970 980 990 1000 1010 DAISRGVPLE EIAKLPVREE IGRMKFEPDI EKIRALIDKT NEQFDELLKK YGA

References

[1]	"The complete sequence of the Pyrococcus furiosus genome." Weiss R.B., Dunn D.M., Robb F.T., Brown J.R. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009950 Genomic DNA. Translation: AAL80306.1.
RefSeq	NP_577911.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1468014.
GenomeReviews	Gene locus PF0182 in contig AE009950_GR.
KEGG	pfu:PF0182.
NMPDR	fig|186497.1.peg.185.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288114.
OMA	HNCGERG.
Enzyme and pathway databases
BRENDA	3.6.3.14. 321.
Family and domain databases
HAMAP	MF_00309. Divergent sequence. [Tree]
InterPro	IPR018118. ATPase_F1/A1-cplx_a/bsu_N. IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C. IPR004100. ATPase_F1/V1/A1-cplx_a/bsu_N. IPR020003. ATPase_F1/V1/A1_a/bsu_AS. IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. [Graphical view]
Pfam	PF00006. ATP-synt_ab. 1 hit. PF00306. ATP-synt_ab_C. 1 hit. PF02874. ATP-synt_ab_N. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
PROSITE	PS00152. ATPASE_ALPHA_BETA. 1 hit. PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VATA_PYRFU

Accession

Primary (citable) accession number: Q8U4A6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	June 1, 2002
Last modified:	December 15, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)