Q8U491 (PURL_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	PF0199

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	704 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 704	704	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100521
Regions
	Nucleotide binding	89 – 100	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8U491 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 9BE12F5AABE25978	FASTA	704	78,229
	10 20 30 40 50 60 MFPHEEKLIR EKLGREPNDL EKAMLEVMWS EHVSYKSSRK WLKLLPTKNE HVILGPGEDA 70 80 90 100 110 120 GIIKFDDKTW IVIGIESHNH PSAVEPYGGA ATGVGGIVRD ILCMGARPIA LLDPIRFGPL 130 140 150 160 170 180 EKEKNRYLFE YVVKGIADYG NRIGVPTVGG ETEFDESLDN YTLVNVACIG IMRPEHLVHS 190 200 210 220 230 240 YVTEPGLKLV IVGNRTGRDG IHGVTFASEE LGENAEEEDR SAVQIPDPFT EKLLIEATLE 250 260 270 280 290 300 AVYTGKVKAL KDLGGGGLTC AASEMVGKRG FGAIIYADKV PLREPGMTPL EVMISESQER 310 320 330 340 350 360 MLFAIKPEDV EELGKIFEKY ELEWSVVGEV IEEPKFIVYW KGRKVAELPI ELLTNVPTIE 370 380 390 400 410 420 WPMKEYRIEE DVETPQISLE EAFEKVWRSP NVISKRWVWE QYDHEVQGRT VIKPGFDSAV 430 440 450 460 470 480 LKINEEYGLA ITADGNPTHC YLNPYHGAMG VVVEVVRNLV SVGAKPLALV DNLNFASPER 490 500 510 520 530 540 PEVYWSFVET IKGLADAAKA FGLAYVSGNV SFYNEVVNKP VKPTPVVAGV GKVKLEKIPR 550 560 570 580 590 600 GPREGDLIGL IGETRKELGG SELYRVLGVS KGIAPRVDLE VEKRNAESVL KLIEEGLVSF 610 620 630 640 650 660 VHDVSRGGVA VALAELSTWF NVGIKSEITT SLLPLDFAFS ESHGRYIVTF PEENLEAVKK 670 680 690 700 IAPITLLGRI GGEKFELKIN GEKVSKTVKW LSDVHWNELY RIMD

References

[1]

"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009950 Genomic DNA. Translation: AAL80323.1.
RefSeq	NP_577928.1. NC_003413.1.
3D structure databases
ProteinModelPortal	Q8U491.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000005246; EBPYRP00000005107; EBPYRG00000005245.
GeneID	1468031.
GenomeReviews	Gene locus PF0199 in contig AE009950_GR.
KEGG	pfu:PF0199.
NMPDR	fig|186497.1.peg.202.
Phylogenomic databases
GeneTree	EBGT00050000022555.
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q8U491.
ProtClustDB	PRK01213.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_PYRFU

Accession

Primary (citable) accession number: Q8U491

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program