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Q8U453 (SYW_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:PF0241
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 385385Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136729

Regions

Motif82 – 909"HIGH" region HAMAP-Rule MF_00140
Motif253 – 2575"KMSKS" region HAMAP-Rule MF_00140

Sequences

Sequence LengthMass (Da)Tools
Q8U453 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 3A7A628958200CCC

FASTA38545,178
        10         20         30         40         50         60 
MEEEFKVTPW EVEGIIDYNK LIEQFGTSPL TDDLLERTAR LTKSELPIFF RRKFFFSHRD 

        70         80         90        100        110        120 
YDKVLDDYEQ GKGFFLYTGR GPSGPMHIGH IIPFFATKWL QEKFDVNLYI QITDDEKFLF 

       130        140        150        160        170        180 
KENLTFEDTK YWAYQNILDI IAVGFDPDKT FIFQNSEFTK IYEMAIPIAK KINFSMAKAV 

       190        200        210        220        230        240 
FGFTEQSKIG MIFFPAIQAA PTFFEKKRCL IPAAIDQDPY WRLQRDFAES LGYYKTAALH 

       250        260        270        280        290        300 
SKFFPPLTGL EGKMSASKPE TAIYLTDNPE EAGKKIWKFA LTGGQPTLKE QREKGGNPEK 

       310        320        330        340        350        360 
CVVFKWLEIF FEPDDKKLME RYYACKNGEL LCGECKRYLI QRVQEFLKEH QEKRKKAEKL 

       370        380 
VEKFKYTGKL AQEQWNKAIP DPLKK 

« Hide

References

[1]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009950 Genomic DNA. Translation: AAL80365.1.
RefSeqNP_577970.1. NC_003413.1.

3D structure databases

ProteinModelPortalQ8U453.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING186497.PF0241.

Proteomic databases

PRIDEQ8U453.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL80365; AAL80365; PF0241.
GeneID1468075.
KEGGpfu:PF0241.

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000224742.
KOK01867.
OMAEFVVTPW.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_A. Trp_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR020653. Tryptophan-tRNA-ligase_arc.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_PYRFU
AccessionPrimary (citable) accession number: Q8U453
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries