Q8U446 (NTPA_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

PF0249

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 185	185	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178277
Regions
	Region	7 – 12	6	Substrate binding By similarity
	Region	65 – 66	2	Substrate binding By similarity
Sites
	Metal binding	36	1	Magnesium or manganese By similarity
	Metal binding	65	1	Magnesium or manganese By similarity
	Binding site	142	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	168	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8U446 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 49CB4C60631C7396	FASTA	185	21,217
	10 20 30 40 50 60 MELFFITSND GKVREAKKFL EPLGINVIKK PLEYPEIQAD TLEDVVVFGL NWLKDKVDKP 70 80 90 100 110 120 FIIEDSGLFI EALNGFPGVY SAYVYKTIGL DGILKLMEGI ENRKAYFKSV IGFYDGEIHL 130 140 150 160 170 180 FVGEVRGRIS NEKRGLHGFG YDPIFVPDGF DKTFAEMSTE EKNSVSHRGK ALKEFYRWMK ENLKK

References

[1]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009950 Genomic DNA. Translation: AAL80373.1.
RefSeq	NP_577978.1. NC_003413.1.
3D structure databases
ProteinModelPortal	Q8U446.
SMR	Q8U446. Positions 1-184.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000004434; EBPYRP00000004295; EBPYRG00000004434.
GeneID	1468083.
GenomeReviews	Gene locus PF0249 in contig AE009950_GR.
KEGG	pfu:PF0249.
NMPDR	fig|186497.1.peg.254.
Phylogenomic databases
GeneTree	EBGT00050000022850.
HOGENOM	HBG697237.
OMA	VEDSGFF.
PhylomeDB	Q8U446.
ProtClustDB	PRK14821.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_PYRFU

Accession

Primary (citable) accession number: Q8U446

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program