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Protein

Inorganic pyrophosphatase

Gene

ppa

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Diphosphate + H2O = 2 phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 4 Mg2+ ions per subunit. Other metal ions can support activity, but at a lower rate. Two Mg2+ ions are required for the activation of the enzyme and are present before substrate binds, two additional Mg2+ ions form complexes with substrate and product.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi66 – 661Magnesium 1UniRule annotation
Metal bindingi71 – 711Magnesium 1UniRule annotation
Metal bindingi71 – 711Magnesium 2UniRule annotation
Metal bindingi103 – 1031Magnesium 1UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inorganic pyrophosphataseUniRule annotation (EC:3.6.1.1UniRule annotation)
Alternative name(s):
Pyrophosphate phospho-hydrolaseUniRule annotation
Short name:
PPaseUniRule annotation
Gene namesi
Name:ppaUniRule annotation
Ordered Locus Names:PF0257
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 178178Inorganic pyrophosphatasePRO_0000137556Add
BLAST

Proteomic databases

PRIDEiQ8U438.

Interactioni

Protein-protein interaction databases

STRINGi186497.PF0257.

Structurei

Secondary structure

1
178
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 64Combined sources
Turni13 – 153Combined sources
Beta strandi16 – 238Combined sources
Beta strandi28 – 336Combined sources
Turni35 – 373Combined sources
Beta strandi40 – 456Combined sources
Beta strandi47 – 493Combined sources
Beta strandi53 – 586Combined sources
Beta strandi71 – 744Combined sources
Beta strandi85 – 9814Combined sources
Beta strandi105 – 1106Combined sources
Helixi114 – 1163Combined sources
Helixi122 – 1243Combined sources
Helixi127 – 13913Combined sources
Helixi142 – 1443Combined sources
Beta strandi148 – 1558Combined sources
Helixi156 – 17217Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TWLX-ray2.20A2-178[»]
ProteinModelPortaliQ8U438.
SMRiQ8U438. Positions 2-173.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U438.

Family & Domainsi

Sequence similaritiesi

Belongs to the PPase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01711. Archaea.
COG0221. LUCA.
HOGENOMiHOG000236472.
KOiK01507.
OMAiPVDYGII.

Family and domain databases

CDDicd00412. pyrophosphatase. 1 hit.
Gene3Di3.90.80.10. 1 hit.
HAMAPiMF_00209. Inorganic_PPase. 1 hit.
InterProiIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERiPTHR10286. PTHR10286. 1 hit.
PfamiPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF50324. SSF50324. 1 hit.
PROSITEiPS00387. PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U438-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPFHDLEPG PDVPEVVYAI IEIPKGSRNK YELDKKTGLL KLDRVLYSPF
60 70 80 90 100
FYPVDYGIIP RTWYEDDDPF DIMVIMREPV YPLTIIEARP IGLFKMIDSG
110 120 130 140 150
DKDYKVLAVP VEDPYFKDWK DIDDVPKAFL DEIAHFFKRY KELQGKEIIV
160 170
EGWEGAEAAK REILRAIEMY KEKFGKKE
Length:178
Mass (Da):20,913
Last modified:June 1, 2002 - v1
Checksum:i5879D3422934C9D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL80381.1.
RefSeqiWP_011011372.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80381; AAL80381; PF0257.
GeneIDi1468091.
KEGGipfu:PF0257.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL80381.1.
RefSeqiWP_011011372.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TWLX-ray2.20A2-178[»]
ProteinModelPortaliQ8U438.
SMRiQ8U438. Positions 2-173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0257.

Proteomic databases

PRIDEiQ8U438.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80381; AAL80381; PF0257.
GeneIDi1468091.
KEGGipfu:PF0257.

Phylogenomic databases

eggNOGiarCOG01711. Archaea.
COG0221. LUCA.
HOGENOMiHOG000236472.
KOiK01507.
OMAiPVDYGII.

Miscellaneous databases

EvolutionaryTraceiQ8U438.

Family and domain databases

CDDicd00412. pyrophosphatase. 1 hit.
Gene3Di3.90.80.10. 1 hit.
HAMAPiMF_00209. Inorganic_PPase. 1 hit.
InterProiIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERiPTHR10286. PTHR10286. 1 hit.
PfamiPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF50324. SSF50324. 1 hit.
PROSITEiPS00387. PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIPYR_PYRFU
AccessioniPrimary (citable) accession number: Q8U438
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.