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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei88SubstrateUniRule annotation1
Metal bindingi231ManganeseUniRule annotation1
Metal bindingi250Manganese; via tele nitrogenUniRule annotation1
Binding sitei272SubstrateUniRule annotation1
Active sitei274UniRule annotation1
Metal bindingi291ManganeseUniRule annotation1
Binding sitei388GTPUniRule annotation1
Binding sitei420GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi273 – 278GTPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:PF0289
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036181 – 624Phosphoenolpyruvate carboxykinase [GTP]Add BLAST624

Proteomic databases

PRIDEiQ8U410.

Interactioni

Protein-protein interaction databases

STRINGi186497.PF0289.

Structurei

3D structure databases

ProteinModelPortaliQ8U410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni222 – 224Substrate bindingUniRule annotation3
Regioni386 – 388Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG05865. Archaea.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiGNHRFAV.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U410-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNEALKKLE ELLDKDQFEK IKAINNPYLH SFLAEWIQWL EPSKVYVCTD
60 70 80 90 100
SEEDEEYVRW KALYYGEERM LEMVRHTVHY DNYYDQARDK QNTKLLVPKG
110 120 130 140 150
TNLPFLNTMD REDGLREIRE IMKGIMRGKE LFIGFFVLGP KNSVFTIPAV
160 170 180 190 200
QLTDSAYVMH SEFLLYRKGY EEFKRLGPTK NFLKFVHSAG ELDERKTSKN
210 220 230 240 250
LDKRRIYIDL VDETVYSANT QYGGNVIGLK KLAFRLTIQR AVREGWLSEH
260 270 280 290 300
MFLMRVNGPN GRKTYFTGAY PSMCGKTSTA MIPWENIVGD DLVFIKNLDG
310 320 330 340 350
VARAVNVEIG VFGIIEGINQ KDDPIIWQVL HSPVEIIFSN VLVKDGKPYW
360 370 380 390 400
NGMGIEIPDE GENHSGKWWR GKRDAEGKEI PPSHKNARFT VRLEAFPNLD
410 420 430 440 450
REALENPCGV EVGGMIFGGR DPDTWPPVRE SFNWDHGVIT MGAALESETT
460 470 480 490 500
AATLGKEGVR AFNPMSILDF LSVHIGDYLR NYLEFGRKLK KTPKIFAVNY
510 520 530 540 550
FLRENGQWLN EKLDKAVWLK WMELRVHNDV GAIETPIGYI PRYEDLKVLF
560 570 580 590 600
RQVLNKDYSR EDYEKQFKIR VPELLAKIDR IWKIYEPIDN IPEELFQELE
610 620
KERKRILEAR EKYGDYISPF ALEK
Length:624
Mass (Da):72,665
Last modified:June 1, 2002 - v1
Checksum:iEC6D925CF97E4C25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL80413.1.
RefSeqiWP_011011403.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80413; AAL80413; PF0289.
GeneIDi1468123.
KEGGipfu:PF0289.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL80413.1.
RefSeqiWP_011011403.1. NC_003413.1.

3D structure databases

ProteinModelPortaliQ8U410.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0289.

Proteomic databases

PRIDEiQ8U410.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80413; AAL80413; PF0289.
GeneIDi1468123.
KEGGipfu:PF0289.

Phylogenomic databases

eggNOGiarCOG05865. Archaea.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiGNHRFAV.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_PYRFU
AccessioniPrimary (citable) accession number: Q8U410
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2002
Last modified: November 11, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.