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Q8U410

- PCKG_PYRFU

UniProt

Q8U410 - PCKG_PYRFU

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei88 – 881SubstrateUniRule annotation
    Binding sitei224 – 2241Substrate; via amide nitrogenUniRule annotation
    Metal bindingi231 – 2311ManganeseUniRule annotation
    Binding sitei231 – 2311SubstrateUniRule annotation
    Metal bindingi250 – 2501Manganese; via tele nitrogenUniRule annotation
    Binding sitei272 – 2721SubstrateUniRule annotation
    Active sitei274 – 2741UniRule annotation
    Metal bindingi291 – 2911ManganeseUniRule annotation
    Binding sitei388 – 3881GTPUniRule annotation
    Binding sitei420 – 4201GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:PF0289
    OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    Taxonomic identifieri186497 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000001013: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 624624Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103618Add
    BLAST

    Proteomic databases

    PRIDEiQ8U410.

    Interactioni

    Protein-protein interaction databases

    STRINGi186497.PF0289.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8U410.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni386 – 3883Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiSEFLLYR.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8U410-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMNEALKKLE ELLDKDQFEK IKAINNPYLH SFLAEWIQWL EPSKVYVCTD    50
    SEEDEEYVRW KALYYGEERM LEMVRHTVHY DNYYDQARDK QNTKLLVPKG 100
    TNLPFLNTMD REDGLREIRE IMKGIMRGKE LFIGFFVLGP KNSVFTIPAV 150
    QLTDSAYVMH SEFLLYRKGY EEFKRLGPTK NFLKFVHSAG ELDERKTSKN 200
    LDKRRIYIDL VDETVYSANT QYGGNVIGLK KLAFRLTIQR AVREGWLSEH 250
    MFLMRVNGPN GRKTYFTGAY PSMCGKTSTA MIPWENIVGD DLVFIKNLDG 300
    VARAVNVEIG VFGIIEGINQ KDDPIIWQVL HSPVEIIFSN VLVKDGKPYW 350
    NGMGIEIPDE GENHSGKWWR GKRDAEGKEI PPSHKNARFT VRLEAFPNLD 400
    REALENPCGV EVGGMIFGGR DPDTWPPVRE SFNWDHGVIT MGAALESETT 450
    AATLGKEGVR AFNPMSILDF LSVHIGDYLR NYLEFGRKLK KTPKIFAVNY 500
    FLRENGQWLN EKLDKAVWLK WMELRVHNDV GAIETPIGYI PRYEDLKVLF 550
    RQVLNKDYSR EDYEKQFKIR VPELLAKIDR IWKIYEPIDN IPEELFQELE 600
    KERKRILEAR EKYGDYISPF ALEK 624
    Length:624
    Mass (Da):72,665
    Last modified:June 1, 2002 - v1
    Checksum:iEC6D925CF97E4C25
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009950 Genomic DNA. Translation: AAL80413.1.
    RefSeqiNP_578018.1. NC_003413.1.
    WP_011011403.1. NC_003413.1.

    Genome annotation databases

    EnsemblBacteriaiAAL80413; AAL80413; PF0289.
    GeneIDi1468123.
    KEGGipfu:PF0289.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009950 Genomic DNA. Translation: AAL80413.1 .
    RefSeqi NP_578018.1. NC_003413.1.
    WP_011011403.1. NC_003413.1.

    3D structure databases

    ProteinModelPortali Q8U410.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 186497.PF0289.

    Proteomic databases

    PRIDEi Q8U410.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL80413 ; AAL80413 ; PF0289 .
    GeneIDi 1468123.
    KEGGi pfu:PF0289.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi SEFLLYR.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
      Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
      Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

    Entry informationi

    Entry nameiPCKG_PYRFU
    AccessioniPrimary (citable) accession number: Q8U410
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 2, 2002
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3