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Q8U410

- PCKG_PYRFU

UniProt

Q8U410 - PCKG_PYRFU

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Protein
Phosphoenolpyruvate carboxykinase [GTP]
Gene
pckG, PF0289
Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881Substrate By similarity
Binding sitei224 – 2241Substrate; via amide nitrogen By similarity
Metal bindingi231 – 2311Manganese By similarity
Binding sitei231 – 2311Substrate By similarity
Metal bindingi250 – 2501Manganese; via tele nitrogen By similarity
Binding sitei272 – 2721Substrate By similarity
Active sitei274 – 2741 By similarity
Metal bindingi291 – 2911Manganese By similarity
Binding sitei388 – 3881GTP By similarity
Binding sitei420 – 4201GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEP carboxykinase
Short name:
PEPCK
Gene namesi
Name:pckG
Ordered Locus Names:PF0289
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 624624Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation
PRO_0000103618Add
BLAST

Proteomic databases

PRIDEiQ8U410.

Interactioni

Protein-protein interaction databases

STRINGi186497.PF0289.

Structurei

3D structure databases

ProteinModelPortaliQ8U410.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni386 – 3883Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiSEFLLYR.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U410-1 [UniParc]FASTAAdd to Basket

« Hide

MMNEALKKLE ELLDKDQFEK IKAINNPYLH SFLAEWIQWL EPSKVYVCTD    50
SEEDEEYVRW KALYYGEERM LEMVRHTVHY DNYYDQARDK QNTKLLVPKG 100
TNLPFLNTMD REDGLREIRE IMKGIMRGKE LFIGFFVLGP KNSVFTIPAV 150
QLTDSAYVMH SEFLLYRKGY EEFKRLGPTK NFLKFVHSAG ELDERKTSKN 200
LDKRRIYIDL VDETVYSANT QYGGNVIGLK KLAFRLTIQR AVREGWLSEH 250
MFLMRVNGPN GRKTYFTGAY PSMCGKTSTA MIPWENIVGD DLVFIKNLDG 300
VARAVNVEIG VFGIIEGINQ KDDPIIWQVL HSPVEIIFSN VLVKDGKPYW 350
NGMGIEIPDE GENHSGKWWR GKRDAEGKEI PPSHKNARFT VRLEAFPNLD 400
REALENPCGV EVGGMIFGGR DPDTWPPVRE SFNWDHGVIT MGAALESETT 450
AATLGKEGVR AFNPMSILDF LSVHIGDYLR NYLEFGRKLK KTPKIFAVNY 500
FLRENGQWLN EKLDKAVWLK WMELRVHNDV GAIETPIGYI PRYEDLKVLF 550
RQVLNKDYSR EDYEKQFKIR VPELLAKIDR IWKIYEPIDN IPEELFQELE 600
KERKRILEAR EKYGDYISPF ALEK 624
Length:624
Mass (Da):72,665
Last modified:June 1, 2002 - v1
Checksum:iEC6D925CF97E4C25
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009950 Genomic DNA. Translation: AAL80413.1.
RefSeqiNP_578018.1. NC_003413.1.
WP_011011403.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80413; AAL80413; PF0289.
GeneIDi1468123.
KEGGipfu:PF0289.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009950 Genomic DNA. Translation: AAL80413.1 .
RefSeqi NP_578018.1. NC_003413.1.
WP_011011403.1. NC_003413.1.

3D structure databases

ProteinModelPortali Q8U410.
ModBasei Search...

Protein-protein interaction databases

STRINGi 186497.PF0289.

Proteomic databases

PRIDEi Q8U410.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL80413 ; AAL80413 ; PF0289 .
GeneIDi 1468123.
KEGGi pfu:PF0289.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi SEFLLYR.

Enzyme and pathway databases

UniPathwayi UPA00138 .

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Entry informationi

Entry nameiPCKG_PYRFU
AccessioniPrimary (citable) accession number: Q8U410
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2002
Last modified: September 3, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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