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Q8U410

- PCKG_PYRFU

UniProt

Q8U410 - PCKG_PYRFU

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn(2+) ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881SubstrateUniRule annotation
Binding sitei224 – 2241Substrate; via amide nitrogenUniRule annotation
Metal bindingi231 – 2311ManganeseUniRule annotation
Binding sitei231 – 2311SubstrateUniRule annotation
Metal bindingi250 – 2501Manganese; via tele nitrogenUniRule annotation
Binding sitei272 – 2721SubstrateUniRule annotation
Active sitei274 – 2741UniRule annotation
Metal bindingi291 – 2911ManganeseUniRule annotation
Binding sitei388 – 3881GTPUniRule annotation
Binding sitei420 – 4201GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:PF0289
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 624624Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103618Add
BLAST

Proteomic databases

PRIDEiQ8U410.

Interactioni

Protein-protein interaction databases

STRINGi186497.PF0289.

Structurei

3D structure databases

ProteinModelPortaliQ8U410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni386 – 3883Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiSEFLLYR.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U410-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMNEALKKLE ELLDKDQFEK IKAINNPYLH SFLAEWIQWL EPSKVYVCTD
60 70 80 90 100
SEEDEEYVRW KALYYGEERM LEMVRHTVHY DNYYDQARDK QNTKLLVPKG
110 120 130 140 150
TNLPFLNTMD REDGLREIRE IMKGIMRGKE LFIGFFVLGP KNSVFTIPAV
160 170 180 190 200
QLTDSAYVMH SEFLLYRKGY EEFKRLGPTK NFLKFVHSAG ELDERKTSKN
210 220 230 240 250
LDKRRIYIDL VDETVYSANT QYGGNVIGLK KLAFRLTIQR AVREGWLSEH
260 270 280 290 300
MFLMRVNGPN GRKTYFTGAY PSMCGKTSTA MIPWENIVGD DLVFIKNLDG
310 320 330 340 350
VARAVNVEIG VFGIIEGINQ KDDPIIWQVL HSPVEIIFSN VLVKDGKPYW
360 370 380 390 400
NGMGIEIPDE GENHSGKWWR GKRDAEGKEI PPSHKNARFT VRLEAFPNLD
410 420 430 440 450
REALENPCGV EVGGMIFGGR DPDTWPPVRE SFNWDHGVIT MGAALESETT
460 470 480 490 500
AATLGKEGVR AFNPMSILDF LSVHIGDYLR NYLEFGRKLK KTPKIFAVNY
510 520 530 540 550
FLRENGQWLN EKLDKAVWLK WMELRVHNDV GAIETPIGYI PRYEDLKVLF
560 570 580 590 600
RQVLNKDYSR EDYEKQFKIR VPELLAKIDR IWKIYEPIDN IPEELFQELE
610 620
KERKRILEAR EKYGDYISPF ALEK
Length:624
Mass (Da):72,665
Last modified:June 1, 2002 - v1
Checksum:iEC6D925CF97E4C25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL80413.1.
RefSeqiNP_578018.1. NC_003413.1.
WP_011011403.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80413; AAL80413; PF0289.
GeneIDi1468123.
KEGGipfu:PF0289.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL80413.1 .
RefSeqi NP_578018.1. NC_003413.1.
WP_011011403.1. NC_003413.1.

3D structure databases

ProteinModelPortali Q8U410.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 186497.PF0289.

Proteomic databases

PRIDEi Q8U410.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL80413 ; AAL80413 ; PF0289 .
GeneIDi 1468123.
KEGGi pfu:PF0289.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi SEFLLYR.

Enzyme and pathway databases

UniPathwayi UPA00138 .

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Entry informationi

Entry nameiPCKG_PYRFU
AccessioniPrimary (citable) accession number: Q8U410
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2002
Last modified: November 26, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3