Reviewed, UniProtKB/Swiss-Prot Q8U3Y2 (GYAR_PYRFU)

Names and origin

Protein names

Recommended name:
    Glyoxylate reductase
    EC=1.1.1.26

Gene names

Name:	gyaR
Ordered Locus Names:	PF0319

Organism

Pyrococcus furiosus [Complete proteome] [HAMAP]

Taxonomic identifier

2261 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Glycolate + NAD+ = glyoxylate + NADH. HAMAP MF_00776
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Glyoxylate reductase HAMAP MF_00776		PRO_0000075948
Regions
	Nucleotide binding	158 – 161	4	NADP By similarity
	Nucleotide binding	180 – 182	3	NADP By similarity
	Nucleotide binding	239 – 241	3	NADP By similarity
	Nucleotide binding	288 – 290	3	NADP By similarity
Sites
	Active site	241	1	By similarity
	Active site	270	1	By similarity
	Active site	288	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8U3Y2-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: CFC48402FD2073B5	FASTA	336	38,342
	10 20 30 40 50 60 MKPKVFITRA IPENGINMLE EEFEVEVWEE EREIPREKLL EKVKDVDALV TMLSERIDQE 70 80 90 100 110 120 VFENAPRLRI VANYAVGYDN IDVEEATRRG IYVTNTPDVL TNATADHAFA LLLATARHVV 130 140 150 160 170 180 KGDKFVRSGE WKRKGIAWHP KWFLGYELYG KTIGIVGFGR IGQAIARRAK GFNMRILYYS 190 200 210 220 230 240 RTRKSQAEKE LGAEYRPLEE VLKESDFVIL AVPLTKETMY MINEERLKLM KPTAILVNIA 250 260 270 280 290 300 RGKVVDTKAL IKALKEGWIA GAGLDVFEEE PYYNEELFSL DNVVLTPHIG SATFEAREAM 310 320 330 AELVARNLIA FKRGEIPPTL VNKEVIKIRK PGFNEQ

References

[1]	"The complete sequence of the Pyrococcus furiosus genome." Weiss R.B., Dunn D.M., Robb F.T., Brown J.R. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010156 Genomic DNA. Translation: AAL80443.1.
RefSeq	NP_578048.1.
3D structure databases
HSSP	HSSP built from PDB template 1GDH based on UniProtKB P36234.
SMR	Q8U3Y2. Positions 1-333.
ModBase	Search...
Genome annotation databases
GeneID	1468154.
GenomeReviews	Gene locus PF0319 in contig AE009950_GR.
KEGG	pfu:PF0319.
NMPDR	fig|186497.1.peg.325.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8U3Y2.
OMA	Q8U3Y2. DFGFALM.
Enzyme and pathway databases
BRENDA	1.1.1.26. 321.
Family and domain databases
HAMAP	MF_00776. [Tree]
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYAR_PYRFU

Accession

Primary (citable) accession number: Q8U3Y2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)