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Reviewed, UniProtKB/Swiss-Prot Q8U3N5 (PUR2_PYRFU)

Last modified November 3, 2009. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase
Gene names
Name: purD
Ordered Locus Names: PF0422
OrganismPyrococcus furiosus [Complete proteome] [HAMAP]
Taxonomic identifier2261 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length439 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 439439Phosphoribosylamine--glycine ligase HAMAP MF_00138
PRO_0000151515

Regions

Domain109 – 317209ATP-grasp
Nucleotide binding136 – 19560ATP By similarity

Sites

Metal binding2751Magnesium or manganese 1 By similarity
Metal binding2871Magnesium or manganese 1 By similarity
Metal binding2871Magnesium or manganese 2 By similarity
Metal binding2891Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8U3N5-1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 7A5CDBB28A94096A

FASTA43948,925
        10         20         30         40         50         60 
MVKVLLVGGG GREHAIGEAL VKGGAELYVV SKHKNPGLAR IAKDYGLARE TDVEKVVEFA 

        70         80         90        100        110        120 
EKWKVDFAFI GPEAPLEAGV VNALEERGIP AVGPTKEAAR LETNKAWARE FMERNKIPGR 

       130        140        150        160        170        180 
KLFRVFDDPK EMKEWIDEFG KPVVVKPLGL TGGKGVKVVG YQLKDNEEAK EYAEYLIRKD 

       190        200        210        220        230        240 
GKVLIEERTD GVEFTFQVFS DGKKVVPMPL AQDYPHAYEG DVGPITGGMG SYSCSNHLLP 

       250        260        270        280        290        300 
FITKSDWEKA LETLQKTVEA MYKEGYPYKG ILYGQFMLSK DGPVIIEYNA RFGDPEAINV 

       310        320        330        340        350        360 
LSILEDNLVE ISERIIDGNL RDVKFSNMAT VVKYIAPQGY PENPIKGVRI EVNEDKIREE 

       370        380        390        400        410        420 
GARLIFASID ENYTLLGSRA LAVVGVSENL EEAEKIAQSA IRHVKGPIFY RKDVGTRESI 

       430 
EKRIKIMKSL RGDFDVNSC 

« Hide

References

[1]"The complete sequence of the Pyrococcus furiosus genome."
Weiss R.B., Dunn D.M., Robb F.T., Brown J.R.
Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases

AE009950 Genomic DNA. Translation: AAL80546.1.
RefSeqNP_578151.1.

3D structure databases

HSSPHSSP built from PDB template 1GSO based on UniProtKB P15640.
ModBaseSearch...

Genome annotation databases

GeneID1468259.
GenomeReviewsGene locus PF0422 in contig AE009950_GR.
KEGGpfu:PF0422.
NMPDRfig|186497.1.peg.430.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ8U3N5.
OMAIEYNCRF.

Enzyme and pathway databases

BRENDA6.3.4.13. 321.

Family and domain databases

HAMAPMF_00138.
[Tree]
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR013817. Pre-ATP_grasp.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N-dom.
[Graphical view]
Gene3DG3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit.
G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_PYRFU
AccessionPrimary (citable) accession number: Q8U3N5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: June 1, 2002
Last modified: November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents