Q8U3N5 (PUR2_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	PF0422

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus furiosus

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138		PRO_0000151515
Regions
	Domain	109 – 317	209	ATP-grasp
	Nucleotide binding	136 – 195	60	ATP By similarity
Sites
	Metal binding	275	1	Magnesium or manganese 1 By similarity
	Metal binding	287	1	Magnesium or manganese 1 By similarity
	Metal binding	287	1	Magnesium or manganese 2 By similarity
	Metal binding	289	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8U3N5 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 7A5CDBB28A94096A	FASTA	439	48,925
	10 20 30 40 50 60 MVKVLLVGGG GREHAIGEAL VKGGAELYVV SKHKNPGLAR IAKDYGLARE TDVEKVVEFA 70 80 90 100 110 120 EKWKVDFAFI GPEAPLEAGV VNALEERGIP AVGPTKEAAR LETNKAWARE FMERNKIPGR 130 140 150 160 170 180 KLFRVFDDPK EMKEWIDEFG KPVVVKPLGL TGGKGVKVVG YQLKDNEEAK EYAEYLIRKD 190 200 210 220 230 240 GKVLIEERTD GVEFTFQVFS DGKKVVPMPL AQDYPHAYEG DVGPITGGMG SYSCSNHLLP 250 260 270 280 290 300 FITKSDWEKA LETLQKTVEA MYKEGYPYKG ILYGQFMLSK DGPVIIEYNA RFGDPEAINV 310 320 330 340 350 360 LSILEDNLVE ISERIIDGNL RDVKFSNMAT VVKYIAPQGY PENPIKGVRI EVNEDKIREE 370 380 390 400 410 420 GARLIFASID ENYTLLGSRA LAVVGVSENL EEAEKIAQSA IRHVKGPIFY RKDVGTRESI 430 EKRIKIMKSL RGDFDVNSC

References

[1]

"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009950 Genomic DNA. Translation: AAL80546.1.
RefSeq	NP_578151.1. NC_003413.1.
3D structure databases
ProteinModelPortal	Q8U3N5.
ModBase	Search...
Protein-protein interaction databases
STRING	186497.PF0422.
Proteomic databases
PRIDE	Q8U3N5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL80546; AAL80546; PF0422.
GeneID	1468259.
KEGG	pfu:PF0422.
Phylogenomic databases
eggNOG	COG0151.
HOGENOM	HOG000033464.
KO	K01945.
OMA	MGAYTPL.
ProtClustDB	PRK00885.
Enzyme and pathway databases
UniPathway	UPA00074; UER00125.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit. 3.90.600.10. 1 hit.
HAMAP	MF_00138. GARS.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp_dom. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_PYRFU

Accession

Primary (citable) accession number: Q8U3N5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program