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Q8U3N5

- PUR2_PYRFU

UniProt

Q8U3N5 - PUR2_PYRFU

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Protein

Phosphoribosylamine--glycine ligase

Gene
purD, PF0422
Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi275 – 2751Magnesium or manganese 1 By similarity
Metal bindingi287 – 2871Magnesium or manganese 1 By similarity
Metal bindingi287 – 2871Magnesium or manganese 2 By similarity
Metal bindingi289 – 2891Magnesium or manganese 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi136 – 19560ATP By similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. manganese ion binding Source: UniProtKB-HAMAP
  4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene namesi
Name:purD
Ordered Locus Names:PF0422
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 439439Phosphoribosylamine--glycine ligaseUniRule annotationPRO_0000151515Add
BLAST

Proteomic databases

PRIDEiQ8U3N5.

Interactioni

Protein-protein interaction databases

STRINGi186497.PF0422.

Structurei

3D structure databases

ProteinModelPortaliQ8U3N5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini109 – 317209ATP-graspAdd
BLAST

Sequence similaritiesi

Belongs to the GARS family.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000033464.
KOiK01945.
OMAiFIDEYDG.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPiMF_00138. GARS.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U3N5-1 [UniParc]FASTAAdd to Basket

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MVKVLLVGGG GREHAIGEAL VKGGAELYVV SKHKNPGLAR IAKDYGLARE    50
TDVEKVVEFA EKWKVDFAFI GPEAPLEAGV VNALEERGIP AVGPTKEAAR 100
LETNKAWARE FMERNKIPGR KLFRVFDDPK EMKEWIDEFG KPVVVKPLGL 150
TGGKGVKVVG YQLKDNEEAK EYAEYLIRKD GKVLIEERTD GVEFTFQVFS 200
DGKKVVPMPL AQDYPHAYEG DVGPITGGMG SYSCSNHLLP FITKSDWEKA 250
LETLQKTVEA MYKEGYPYKG ILYGQFMLSK DGPVIIEYNA RFGDPEAINV 300
LSILEDNLVE ISERIIDGNL RDVKFSNMAT VVKYIAPQGY PENPIKGVRI 350
EVNEDKIREE GARLIFASID ENYTLLGSRA LAVVGVSENL EEAEKIAQSA 400
IRHVKGPIFY RKDVGTRESI EKRIKIMKSL RGDFDVNSC 439
Length:439
Mass (Da):48,925
Last modified:June 1, 2002 - v1
Checksum:i7A5CDBB28A94096A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009950 Genomic DNA. Translation: AAL80546.1.
RefSeqiNP_578151.1. NC_003413.1.
WP_011011536.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80546; AAL80546; PF0422.
GeneIDi1468259.
KEGGipfu:PF0422.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009950 Genomic DNA. Translation: AAL80546.1 .
RefSeqi NP_578151.1. NC_003413.1.
WP_011011536.1. NC_003413.1.

3D structure databases

ProteinModelPortali Q8U3N5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 186497.PF0422.

Proteomic databases

PRIDEi Q8U3N5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL80546 ; AAL80546 ; PF0422 .
GeneIDi 1468259.
KEGGi pfu:PF0422.

Phylogenomic databases

eggNOGi COG0151.
HOGENOMi HOG000033464.
KOi K01945.
OMAi FIDEYDG.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPi MF_00138. GARS.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR00877. purD. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Entry informationi

Entry nameiPUR2_PYRFU
AccessioniPrimary (citable) accession number: Q8U3N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: June 1, 2002
Last modified: September 3, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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