Q8U3M6 (PUR5_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	PF0431

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_A
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00741_A.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_A		PRO_0000148286

Sequences

	Sequence		Length	Mass (Da)
	Q8U3M6 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 981D538B9DE814FF	FASTA	334	36,754
	10 20 30 40 50 60 MLTYAQAGVD EEKTSKALRF IIEAARKTFE FRKGKLGEPG DIGHYSALLD FGNYYLAITT 70 80 90 100 110 120 DGVGTKVLVA EAVGKFDTIG IDMIAMNVND LICVGAEPIA LVDYFAIKEP NERVFEEVAK 130 140 150 160 170 180 GLYKGAKEAG IAIVGGETAV MPDLVNGYDL AGTAIGIVEK DKVITGEKIR PGDAVIGISS 190 200 210 220 230 240 SGIHSNGLTL ARKLLIPKYG LDYEYESRKL WEWLLEPTRI YVKPILELLK SVEVHGLAHI 250 260 270 280 290 300 TGGGLLNLKR ITNYGFRLNM PPINGIFKLI HENGVPLEEM FRVFNMGVGF VVVVSQEDKE 310 320 330 EALQILNRYY ESFELGTVSE RPGIIVENYG VKFI

References

[1]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009950 Genomic DNA. Translation: AAL80555.1.
RefSeq	NP_578160.1. NC_003413.1.
3D structure databases
ProteinModelPortal	Q8U3M6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000005896; EBPYRP00000005757; EBPYRG00000005895.
GeneID	1468269.
GenomeReviews	Gene locus PF0431 in contig AE009950_GR.
KEGG	pfu:PF0431.
NMPDR	fig|186497.1.peg.440.
Phylogenomic databases
GeneTree	EBGT00050000022555.
HOGENOM	HBG531222.
OMA	GIDMIAM.
PhylomeDB	Q8U3M6.
ProtClustDB	PRK05385.
Family and domain databases
HAMAP	MF_00741_A. AIRS_A. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
KO	K01933.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. PurM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_PYRFU

Accession

Primary (citable) accession number: Q8U3M6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program