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Protein

Thermostable carboxypeptidase 1

Gene

PF0456

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues, but not Pro, Gly, Asp and Glu.1 Publication

Catalytic activityi

Release of a C-terminal amino acid with broad specificity, except for -Pro.1 Publication

Cofactori

Co2+2 Publications, Mn2+2 PublicationsNote: Binds 1 cobalt ion per subunit. Can also utilize Mn2+ (in vitro). Is not active with zinc ions.2 Publications

Enzyme regulationi

EDTA and DTT reversibly abolish carboxypeptidase activity.1 Publication

pH dependencei

Optimum pH is 6.2-6.6.1 Publication

Temperature dependencei

Optimum temperature is 90-100 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi269Cobalt; catalytic1 Publication1
Active sitei270Proton donor/acceptorBy similarity1
Metal bindingi273Cobalt; catalytic1 Publication1
Metal bindingi299Cobalt1 Publication1

GO - Molecular functioni

  • cobalt ion binding Source: UniProtKB
  • metallocarboxypeptidase activity Source: UniProtKB

GO - Biological processi

  • proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Cobalt, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.17.B5. 5243.

Protein family/group databases

MEROPSiM32.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermostable carboxypeptidase 1 (EC:3.4.17.19)
Alternative name(s):
Carboxypeptidase Pfu
Short name:
PfuCP
Gene namesi
Ordered Locus Names:PF0456
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004288341 – 499Thermostable carboxypeptidase 1Add BLAST499

Proteomic databases

PRIDEiQ8U3L0.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi186497.PF0456.

Structurei

Secondary structure

1499
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 36Combined sources29
Helixi40 – 42Combined sources3
Helixi43 – 61Combined sources19
Helixi64 – 74Combined sources11
Helixi81 – 99Combined sources19
Helixi102 – 124Combined sources23
Helixi128 – 130Combined sources3
Helixi132 – 149Combined sources18
Helixi155 – 163Combined sources9
Helixi169 – 192Combined sources24
Helixi201 – 203Combined sources3
Helixi209 – 223Combined sources15
Turni228 – 230Combined sources3
Beta strandi231 – 235Combined sources5
Beta strandi241 – 245Combined sources5
Beta strandi248 – 253Combined sources6
Helixi261 – 278Combined sources18
Helixi282 – 284Combined sources3
Helixi295 – 306Combined sources12
Turni307 – 311Combined sources5
Helixi313 – 326Combined sources14
Helixi328 – 332Combined sources5
Helixi335 – 342Combined sources8
Helixi351 – 353Combined sources3
Turni356 – 358Combined sources3
Helixi359 – 375Combined sources17
Helixi380 – 382Combined sources3
Helixi383 – 395Combined sources13
Helixi402 – 404Combined sources3
Turni405 – 407Combined sources3
Helixi411 – 414Combined sources4
Helixi421 – 440Combined sources20
Helixi444 – 450Combined sources7
Helixi454 – 463Combined sources10
Helixi465 – 467Combined sources3
Helixi473 – 481Combined sources9
Helixi488 – 498Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K9XX-ray2.30A/B/C/D1-499[»]
1KA2X-ray2.20A1-499[»]
1KA4X-ray3.00A1-499[»]
ProteinModelPortaliQ8U3L0.
SMRiQ8U3L0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U3L0.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M32 family.Curated

Phylogenomic databases

eggNOGiarCOG04247. Archaea.
COG2317. LUCA.
HOGENOMiHOG000040803.
KOiK01299.
OMAiEFGHALY.

Family and domain databases

CDDicd06460. M32_Taq. 1 hit.
InterProiIPR001333. Peptidase_M32_Taq.
[Graphical view]
PfamiPF02074. Peptidase_M32. 1 hit.
[Graphical view]
PIRSFiPIRSF006615. Zn_crbxpep_Taq. 1 hit.
PRINTSiPR00998. CRBOXYPTASET.

Sequencei

Sequence statusi: Complete.

Q8U3L0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEVFQNETI KQILAKYRRI WAIGHAQSVL GWDLEVNMPK EGILERSVAQ
60 70 80 90 100
GELSVLSHEL LLHPEFVNLV EKAKGLENLN EYERGIVRVL DRSIRIARAF
110 120 130 140 150
PPEFIREVSE TTSLATKAWE EAKAKDDFSK FEPWLDKIIS LAKRAAEYLG
160 170 180 190 200
YEEEPYDALL DLYEEGLRTR DVEKMFEVLE KKLKPLLDKI LEEGKVPREH
210 220 230 240 250
PLEKEKYERE WMERVNLWIL QKFGFPLGTR ARLDVSAHPF TTEFGIRDVR
260 270 280 290 300
ITTRYEGYDF RRTILSTVHE FGHALYELQQ DERFMFTPIA GGVSLGIHES
310 320 330 340 350
QSRFWENIIG RSKEFVELIY PVLKENLPFM SNYTPEDVYL YFNIVRPDFI
360 370 380 390 400
RTEADVVTYN FHILLRFKLE RLMVSEEIKA KDLPEMWNDE MERLLGIRPR
410 420 430 440 450
KYSEGILQDI HWAHGSIGYF PTYTIGTLLS AQLYYHIKKD IPDFEEKVAK
460 470 480 490
AEFDPIKAWL REKIHRWGSI YPPKELLKKA IGEDMDAEYF VRWVKEKYL
Length:499
Mass (Da):59,043
Last modified:June 1, 2002 - v1
Checksum:i627A8347CD899BC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL80580.1.
RefSeqiWP_011011573.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80580; AAL80580; PF0456.
GeneIDi1468297.
KEGGipfu:PF0456.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL80580.1.
RefSeqiWP_011011573.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K9XX-ray2.30A/B/C/D1-499[»]
1KA2X-ray2.20A1-499[»]
1KA4X-ray3.00A1-499[»]
ProteinModelPortaliQ8U3L0.
SMRiQ8U3L0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0456.

Protein family/group databases

MEROPSiM32.002.

Proteomic databases

PRIDEiQ8U3L0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80580; AAL80580; PF0456.
GeneIDi1468297.
KEGGipfu:PF0456.

Phylogenomic databases

eggNOGiarCOG04247. Archaea.
COG2317. LUCA.
HOGENOMiHOG000040803.
KOiK01299.
OMAiEFGHALY.

Enzyme and pathway databases

BRENDAi3.4.17.B5. 5243.

Miscellaneous databases

EvolutionaryTraceiQ8U3L0.

Family and domain databases

CDDicd06460. M32_Taq. 1 hit.
InterProiIPR001333. Peptidase_M32_Taq.
[Graphical view]
PfamiPF02074. Peptidase_M32. 1 hit.
[Graphical view]
PIRSFiPIRSF006615. Zn_crbxpep_Taq. 1 hit.
PRINTSiPR00998. CRBOXYPTASET.
ProtoNetiSearch...

Entry informationi

Entry nameiCBP1_PYRFU
AccessioniPrimary (citable) accession number: Q8U3L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.