Q8U3I5 (IF2B_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translation initiation factor 2 subunit beta

Alternative name(s):
aIF2-beta
eIF-2-beta

Gene names

Name:	eif2b
Ordered Locus Names:	PF0481

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus furiosus

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA By similarity. HAMAP-Rule MF_00232
Subunit structure	Heterotrimer composed of an alpha, a beta and a gamma chain By similarity.
Sequence similarities	Belongs to the eIF-2-beta/eIF-5 family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Initiation factor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 140

140

Translation initiation factor 2 subunit beta HAMAP-Rule MF_00232

PRO_0000137430

Secondary structure

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140

Helix Strand Turn

Details...

Helix

8 – 13

6

Turn

14 – 16

3

Beta strand

17 – 19

3

Helix

21 – 24

4

Beta strand

25 – 27

3

Beta strand

37 – 40

4

Beta strand

43 – 47

5

Helix

49 – 56

8

Helix

61 – 70

10

Beta strand

75 – 78

4

Beta strand

81 – 86

6

Helix

90 – 104

15

Beta strand

108 – 110

3

Beta strand

119 – 124

6

Beta strand

129 – 131

3

Sequences

	Sequence		Length	Mass (Da)
	Q8U3I5 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 771153C98A871359	FASTA	140	16,234
	10 20 30 40 50 60 MEIDYYDYEK LLEKAYQELP ENVKHHKSRF EVPGALVTIE GNKTIIENFK DIADALNRDP 70 80 90 100 110 120 QHLLKFLLRE IATAGTLEGR RVVLQGRFTP YLIANKLKKY IKEYVICPVC GSPDTKIIKR 130 140 DRFHFLKCEA CGAETPIQHL

References

[1]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE009950 Genomic DNA. Translation: AAL80605.1.

RefSeq

NP_578210.1. NC_003413.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2D74	X-ray	2.80	B	1-140	[»]
2DCU	X-ray	3.40	B	1-140	[»]

ProteinModelPortal

Q8U3I5.

SMR

Q8U3I5. Positions 3-139.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8U3I5. 1 interaction.

STRING

186497.PF0481.

Proteomic databases

PRIDE

Q8U3I5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAL80605; AAL80605; PF0481.

GeneID

1468322.

KEGG

pfu:PF0481.

Phylogenomic databases

eggNOG

COG1601.

HOGENOM

HOG000107197.

KO

K03238.

OMA

CNSIDTE.

ProtClustDB

PRK03988.

Family and domain databases

Gene3D

3.30.30.50. 1 hit.

HAMAP

MF_00232. eIF-2-beta.

InterPro

IPR004458. TIF2_bsu_arc.
IPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
[Graphical view]

Pfam

PF01873. eIF-5_eIF-2B. 1 hit.
[Graphical view]

SMART

SM00653. eIF2B_5. 1 hit.
[Graphical view]

SUPFAM

SSF100966. Transl_init_fac_IF2/IF5_N. 1 hit.
SSF75689. Transl_init_fac_IF2/IF5_Zn-bd. 1 hit.

TIGRFAMs

TIGR00311. aIF-2beta. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q8U3I5.

Entry information

Entry name

IF2B_PYRFU

Accession

Primary (citable) accession number: Q8U3I5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program