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Q8U2E6

- SYL_PYRFU

UniProt

Q8U2E6 - SYL_PYRFU

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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei653 – 6531ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:PF0890
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 967967Leucine--tRNA ligasePRO_0000152139Add
BLAST

Proteomic databases

PRIDEiQ8U2E6.

Interactioni

Protein-protein interaction databases

STRINGi186497.PF0890.

Structurei

3D structure databases

ProteinModelPortaliQ8U2E6.
SMRiQ8U2E6. Positions 4-967.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi650 – 6545"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiIDKWLLH.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U2E6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPELNFKAIE EKWQKRWLEA KIFEPNIKDK PKEKKFYITV AFPYLSGHLH
60 70 80 90 100
VGHARTYTIP DVIARFKRMQ GYNVLFPMAW HITGSPIVGI AERIKNRDPQ
110 120 130 140 150
TIWIYRDVYK VPEDILWTFE DPVNIVKYFM KAAKETFIRA GFSVDWSREF
160 170 180 190 200
YTTSLFPPFS KFIEWQFWKL KEKGYIVKGT HRVRWDPVVG TPLGDHDLMD
210 220 230 240 250
GEDVPILEYI LIKFELKEGE ETVYLPAATL RPETVYGVTN MWLNPEATYV
260 270 280 290 300
KAKVKKGDKV ETWIISKEAA YKLSFQDREI EVVEEFKGEK LIGKFVRNPV
310 320 330 340 350
TGDEVIILPA EFVDPDNATG VVMSVPAHAP FDHVALEDLK KQTDILLKYD
360 370 380 390 400
IDPRIVENIT YISLIKLEGY GEFPAVEEVQ KLGVKSQKDK DKLEQATKTI
410 420 430 440 450
YRAEYHKGIF KIPPYEGKPV SEVKELIAKD LMEKGIGEIM YEFAEKNVIS
460 470 480 490 500
RFGNRAVIKI IHDQWFIDYG NPEWKEKARE ALKRMKILPE SRRAQFEAII
510 520 530 540 550
EWLDKKACAR KVGLGTPLPW DPDWVIESLS DSTIYMAYYT ISRHINKLRE
560 570 580 590 600
EGKLDPEKLT PEFFDYIFLE EFDEKKEKEL EEKTGISAEI IHEMKEEFEY
610 620 630 640 650
WYPLDWRCSA KDLIPNHLTF FIFNHVAIFP EKHWPKGIAV NGFGTLEGQK
660 670 680 690 700
MSKSKGNVLN FIDAIEENGA DVVRLYIMSL AEHDSDFDWR RKEVGRLRKQ
710 720 730 740 750
IERFYELISQ FAEYEARENV ELKDIDKWML HRLNKAIKGT TDALEEFRTR
760 770 780 790 800
TAVQWAFYTI MNDLRWYLRR TEGRDDEAKR YVLRTLADIW VRLMAPFTPH
810 820 830 840 850
ICEELWEKLG GEGFVSLAKW PDPVEEWWNE TIEAEEEYVK SVMEDIKEII
860 870 880 890 900
EVAKIENARR AYIYTAEDWK WKVVEVVAEK RDFKAAMSEL MKDQELRKRG
910 920 930 940 950
KEIAKIVERL IKDRAFEVKR IDEEKVLREA KDFIEKELGI EIIINPSEDK
960
GGKKKQAMPL KPAIFIE
Length:967
Mass (Da):113,720
Last modified:June 1, 2002 - v1
Checksum:iADABF8E6A4FAD7E7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009950 Genomic DNA. Translation: AAL81014.1.
RefSeqiNP_578619.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81014; AAL81014; PF0890.
GeneIDi1468752.
KEGGipfu:PF0890.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009950 Genomic DNA. Translation: AAL81014.1 .
RefSeqi NP_578619.1. NC_003413.1.

3D structure databases

ProteinModelPortali Q8U2E6.
SMRi Q8U2E6. Positions 4-967.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 186497.PF0890.

Proteomic databases

PRIDEi Q8U2E6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL81014 ; AAL81014 ; PF0890 .
GeneIDi 1468752.
KEGGi pfu:PF0890.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000216620.
KOi K01869.
OMAi IDKWLLH.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_A. Leu_tRNA_synth_A.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsi TIGR00395. leuS_arch. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Entry informationi

Entry nameiSYL_PYRFU
AccessioniPrimary (citable) accession number: Q8U2E6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3