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Q8U2E6 (SYL_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PF0890
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length967 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 967967Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152139

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif650 – 6545"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6531ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8U2E6 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: ADABF8E6A4FAD7E7

FASTA967113,720
        10         20         30         40         50         60 
MPELNFKAIE EKWQKRWLEA KIFEPNIKDK PKEKKFYITV AFPYLSGHLH VGHARTYTIP 

        70         80         90        100        110        120 
DVIARFKRMQ GYNVLFPMAW HITGSPIVGI AERIKNRDPQ TIWIYRDVYK VPEDILWTFE 

       130        140        150        160        170        180 
DPVNIVKYFM KAAKETFIRA GFSVDWSREF YTTSLFPPFS KFIEWQFWKL KEKGYIVKGT 

       190        200        210        220        230        240 
HRVRWDPVVG TPLGDHDLMD GEDVPILEYI LIKFELKEGE ETVYLPAATL RPETVYGVTN 

       250        260        270        280        290        300 
MWLNPEATYV KAKVKKGDKV ETWIISKEAA YKLSFQDREI EVVEEFKGEK LIGKFVRNPV 

       310        320        330        340        350        360 
TGDEVIILPA EFVDPDNATG VVMSVPAHAP FDHVALEDLK KQTDILLKYD IDPRIVENIT 

       370        380        390        400        410        420 
YISLIKLEGY GEFPAVEEVQ KLGVKSQKDK DKLEQATKTI YRAEYHKGIF KIPPYEGKPV 

       430        440        450        460        470        480 
SEVKELIAKD LMEKGIGEIM YEFAEKNVIS RFGNRAVIKI IHDQWFIDYG NPEWKEKARE 

       490        500        510        520        530        540 
ALKRMKILPE SRRAQFEAII EWLDKKACAR KVGLGTPLPW DPDWVIESLS DSTIYMAYYT 

       550        560        570        580        590        600 
ISRHINKLRE EGKLDPEKLT PEFFDYIFLE EFDEKKEKEL EEKTGISAEI IHEMKEEFEY 

       610        620        630        640        650        660 
WYPLDWRCSA KDLIPNHLTF FIFNHVAIFP EKHWPKGIAV NGFGTLEGQK MSKSKGNVLN 

       670        680        690        700        710        720 
FIDAIEENGA DVVRLYIMSL AEHDSDFDWR RKEVGRLRKQ IERFYELISQ FAEYEARENV 

       730        740        750        760        770        780 
ELKDIDKWML HRLNKAIKGT TDALEEFRTR TAVQWAFYTI MNDLRWYLRR TEGRDDEAKR 

       790        800        810        820        830        840 
YVLRTLADIW VRLMAPFTPH ICEELWEKLG GEGFVSLAKW PDPVEEWWNE TIEAEEEYVK 

       850        860        870        880        890        900 
SVMEDIKEII EVAKIENARR AYIYTAEDWK WKVVEVVAEK RDFKAAMSEL MKDQELRKRG 

       910        920        930        940        950        960 
KEIAKIVERL IKDRAFEVKR IDEEKVLREA KDFIEKELGI EIIINPSEDK GGKKKQAMPL 


KPAIFIE 

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References

[1]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009950 Genomic DNA. Translation: AAL81014.1.
RefSeqNP_578619.1. NC_003413.1.

3D structure databases

ProteinModelPortalQ8U2E6.
SMRQ8U2E6. Positions 4-967.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING186497.PF0890.

Proteomic databases

PRIDEQ8U2E6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL81014; AAL81014; PF0890.
GeneID1468752.
KEGGpfu:PF0890.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAIDKWLLH.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PYRFU
AccessionPrimary (citable) accession number: Q8U2E6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries