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Protein

Sulfhydrogenase 1 subunit beta

Gene

hydB

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of a bifunctional enzyme complex that functions as an NADPH-dependent hydrogen-evolving hydrogenase with sulfur reducing activity. May play a role in hydrogen cycling during fermentative growth. Activity not exhibited with NAD. The beta and gamma subunits form the sulfur reducing component that catalyzes the cytoplasmic production of hydrogen sulfide in the presence of elemental sulfur. Not active in the presence of sodium sulfate, sodium sulfite, sodium thiosulfate or cysteine.4 Publications

Catalytic activityi

H2 + polysulfide(n) = hydrogen sulfide + polysulfide(n-1).2 Publications

Cofactori

[4Fe-4S] cluster2 PublicationsNote: Binds 2 [4Fe-4S] clusters.2 Publications

Enzyme regulationi

Stimulated by rubredoxin at pH 7.6 but not ferredoxin.1 Publication

Kineticsi

Measured for the whole complex.1 Publication

Manual assertion based on experiment ini

    1. Vmax=6 µmol/min/mg enzyme2 Publications

    pH dependencei

    Optimum pH is 8.4 for sulfur reductase activity at 80 degrees Celsius.2 Publications

    Temperature dependencei

    Optimum temperature is 80 degrees Celsius for maximal sulfur reductase activity. Activity increases linearly from above 30 degrees Celsius to reach maximal levels at 80 degrees Celsius and then decreases to 15% of activity at 90 degrees Celsius.2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi233Iron-sulfur 1 (4Fe-4S)By similarity1
    Metal bindingi236Iron-sulfur 1 (4Fe-4S)By similarity1
    Metal bindingi239Iron-sulfur 1 (4Fe-4S)By similarity1
    Metal bindingi243Iron-sulfur 1 (4Fe-4S)By similarity1
    Metal bindingi312Iron-sulfur 2 (4Fe-4S)By similarity1
    Metal bindingi315Iron-sulfur 2 (4Fe-4S)By similarity1
    Metal bindingi318Iron-sulfur 2 (4Fe-4S)By similarity1
    Metal bindingi322Iron-sulfur 2 (4Fe-4S)By similarity1

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BRENDAi1.12.98.4. 5243.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sulfhydrogenase 1 subunit beta (EC:1.12.98.4)
    Alternative name(s):
    Sulfhydrogenase I subunit beta1 Publication
    Sulfur reductase subunit HydB1 Publication
    Gene namesi
    Name:hydB1 Publication
    Ordered Locus Names:PF0891
    OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    Taxonomic identifieri186497 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    Proteomesi
    • UP000001013 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004207231 – 367Sulfhydrogenase 1 subunit betaAdd BLAST367

    Interactioni

    Subunit structurei

    Heterotetramer of alpha, beta, gamma and delta subunits. The nickel-containing alpha and delta subunits constitute the hydrogenase activity. The beta and gamma subunits (flavin-containing dimer) constitute the sulfur reductase activity.3 Publications

    Protein-protein interaction databases

    STRINGi186497.PF0891.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8U2E5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini222 – 2544Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST33
    Domaini303 – 3324Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST30

    Sequence similaritiesi

    Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiarCOG05128. Archaea.
    COG1145. LUCA.
    HOGENOMiHOG000227558.
    KOiK17996.
    OMAiRWRQRIM.

    Family and domain databases

    InterProiIPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    [Graphical view]
    PfamiPF17179. Fer4_22. 1 hit.
    [Graphical view]
    PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8U2E5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRYVKLPKEN TYEFLERLKD WGKLYAPVKI SDKFYDFREI DDVRKIEFHY
    60 70 80 90 100
    NRTIMPPKKF FFKPREKLFE FDISKPEYRE VIEEVEPFII FGVHACDIYG
    110 120 130 140 150
    LKILDTVYLD EFPDKYYKVR REKGIIIGIS CMPDEYCFCN LRETDFADDG
    160 170 180 190 200
    FDLFFHELPD GWLVRVGTPT GHRLVDKNIK LFEEVTDKDI CAFRDFEKRR
    210 220 230 240 250
    QQAFKYHEDW GNLRYLLELE MEHPMWDEEA DKCLACGICN TTCPTCRCYE
    260 270 280 290 300
    VQDIVNLDGV TGYRERRWDS CQFRSHGLVA GGHNFRPTKK DRFRNRYLCK
    310 320 330 340 350
    NAYNEKLGLS YCVGCGRCTA FCPANISFVG NLRRILGLEE NKCPPTVSEE
    360
    IPKRGFAYSS NIRGDGV
    Length:367
    Mass (Da):43,399
    Last modified:June 1, 2002 - v1
    Checksum:i8329F3842CBC48D0
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti205K → Q in CAA53034 (PubMed:7704275).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X75255 Genomic DNA. Translation: CAA53034.1.
    AE009950 Genomic DNA. Translation: AAL81015.1.
    RefSeqiWP_011012026.1. NC_003413.1.

    Genome annotation databases

    EnsemblBacteriaiAAL81015; AAL81015; PF0891.
    GeneIDi1468753.
    KEGGipfu:PF0891.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X75255 Genomic DNA. Translation: CAA53034.1.
    AE009950 Genomic DNA. Translation: AAL81015.1.
    RefSeqiWP_011012026.1. NC_003413.1.

    3D structure databases

    ProteinModelPortaliQ8U2E5.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi186497.PF0891.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAL81015; AAL81015; PF0891.
    GeneIDi1468753.
    KEGGipfu:PF0891.

    Phylogenomic databases

    eggNOGiarCOG05128. Archaea.
    COG1145. LUCA.
    HOGENOMiHOG000227558.
    KOiK17996.
    OMAiRWRQRIM.

    Enzyme and pathway databases

    BRENDAi1.12.98.4. 5243.

    Family and domain databases

    InterProiIPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    [Graphical view]
    PfamiPF17179. Fer4_22. 1 hit.
    [Graphical view]
    PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiHYD1B_PYRFU
    AccessioniPrimary (citable) accession number: Q8U2E5
    Secondary accession number(s): Q59667
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2013
    Last sequence update: June 1, 2002
    Last modified: September 7, 2016
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.