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Protein

N-glycosylase/DNA lyase

Gene

PF0904

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei268-oxoguanineUniRule annotation1
Binding sitei538-oxoguanine; via carbonyl oxygenUniRule annotation1
Binding sitei648-oxoguanineUniRule annotation1
Active sitei144Schiff-base intermediate with DNAUniRule annotation1
Binding sitei1488-oxoguanineUniRule annotation1
Binding sitei1748-oxoguanine; via carbonyl oxygenUniRule annotation1
Active sitei176UniRule annotation1
Binding sitei2108-oxoguanineUniRule annotation1
Binding sitei2148-oxoguanineUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
Biological processDNA damage, DNA excision, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
N-glycosylase/DNA lyaseUniRule annotation
Alternative name(s):
8-oxoguanine DNA glycosylaseUniRule annotation (EC:3.2.2.-UniRule annotation)
AGOGUniRule annotation
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation (EC:4.2.99.18UniRule annotation)
Short name:
AP lyaseUniRule annotation
Gene namesi
Ordered Locus Names:PF0904
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001851141 – 242N-glycosylase/DNA lyaseAdd BLAST242

Proteomic databases

PRIDEiQ8U2D5

Interactioni

Protein-protein interaction databases

STRINGi186497.PF0904

Structurei

Secondary structure

1242
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 22Combined sources21
Helixi25 – 34Combined sources10
Helixi39 – 51Combined sources13
Helixi60 – 71Combined sources12
Helixi79 – 86Combined sources8
Turni87 – 89Combined sources3
Helixi97 – 112Combined sources16
Helixi116 – 124Combined sources9
Helixi126 – 137Combined sources12
Helixi144 – 160Combined sources17
Helixi177 – 184Combined sources8
Helixi191 – 202Combined sources12
Helixi206 – 217Combined sources12
Helixi227 – 239Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XG7X-ray1.88A/B2-242[»]
4PIIX-ray2.17A2-242[»]
ProteinModelPortaliQ8U2D5
SMRiQ8U2D5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U2D5

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni120 – 184Helix-hairpin-helixAdd BLAST65

Domaini

Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).

Sequence similaritiesi

Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04144 Archaea
COG4047 LUCA
HOGENOMiHOG000254352
KOiK01741
OMAiVKMFGYA
OrthoDBiPOG093Z09FD

Family and domain databases

Gene3Di1.10.1670.10, 2 hits
HAMAPiMF_01168 AGOG, 1 hit
InterProiView protein in InterPro
IPR016544 AGOG
IPR015254 AGOG-like
IPR011257 DNA_glycosylase
IPR023170 HTH_base_excis_C
PfamiView protein in Pfam
PF09171 AGOG, 1 hit
PIRSFiPIRSF008955 AGOG, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit

Sequencei

Sequence statusi: Complete.

Q8U2D5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRELVEIIKG IGIEGAKEVE EKVDRQFYAL QYLFRHQDPE MFIKLVIANS
60 70 80 90 100
LVSYQLTGRG EDWWWEFARY FSGREVDSIW KAYGEFLPKS KNNRRLIEAK
110 120 130 140 150
LNRIRKVEGF LSTLTLKDLE GYYKNMKMLW KALIKIMGSR EDSKTIVFTV
160 170 180 190 200
KMFGYASRIA FSRFIPYPME IPIPEDLRIK SVTSKLTQEK PTKFWMKIGQ
210 220 230 240
ESGVPPLHID SLIWPLLGNA DLTPLDIELR NKLMKLTELL GL
Length:242
Mass (Da):28,345
Last modified:June 1, 2002 - v1
Checksum:i6FA190D348D3E823
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA Translation: AAL81028.1
RefSeqiWP_014835272.1, NC_003413.1

Genome annotation databases

EnsemblBacteriaiAAL81028; AAL81028; PF0904
GeneIDi1468768
KEGGipfu:PF0904
PATRICifig|186497.12.peg.959

Similar proteinsi

Entry informationi

Entry nameiAGOG_PYRFU
AccessioniPrimary (citable) accession number: Q8U2D5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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