Q8U2A3 (ILVC_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	PF0936

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000151398
Sites
	Active site	111	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8U2A3 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: E3DABA7301145794	FASTA	332	37,293
	10 20 30 40 50 60 MVRVVKVWYD NEVSMDILKD KTVAVIGYGS QGEAQAKNMR DSGVHVILGL RPEGNSWKRA 70 80 90 100 110 120 QKDGFEVYTI EEATKRADIV HILIPDLVQP KVYKEKIAPY LREGQALGFS HGFNIHYKQI 130 140 150 160 170 180 VPPEYVDVIM VAPKSPGRRV REVFLEGFGV PALIAVYQDY TGHAKELALA MAKAIGATRA 190 200 210 220 230 240 GVIETTFKDE TESDLIGEQL VLVGGLIELI KKGFEVLVEL GYPPELAYFE ACNEAKLIMD 250 260 270 280 290 300 LIYERGFTGM LKAVSDTAKY GGLTVGPKVI DEHVKENMRK YAERVRSGEF AKEWISKADK 310 320 330 ADEVLKELMK EIEEHEIEKV GRFIRRMSGL EK

References

[1]

"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009950 Genomic DNA. Translation: AAL81060.1.
RefSeq	NP_578665.1. NC_003413.1.
3D structure databases
ProteinModelPortal	Q8U2A3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000004939; EBPYRP00000004800; EBPYRG00000004938.
GeneID	1468800.
GenomeReviews	Gene locus PF0936 in contig AE009950_GR.
KEGG	pfu:PF0936.
NMPDR	fig|186497.1.peg.971.
Phylogenomic databases
GeneTree	EBGT00050000023153.
HOGENOM	HBG286525.
OMA	MAYAKGI.
PhylomeDB	Q8U2A3.
ProtClustDB	PRK05479.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_PYRFU

Accession

Primary (citable) accession number: Q8U2A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program