Q8U262 (RIFK_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	PF0988

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Riboflavin kinase HAMAP MF_01285		PRO_0000322102
Regions
	Nucleotide binding	97 – 102	6	CDP By similarity
	Nucleotide binding	193 – 196	4	CDP By similarity
	Region	1 – 87	87	H-T-H motif-like HAMAP MF_01285
	Region	88 – 212	125	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	124	1	Magnesium By similarity
	Metal binding	126	1	Magnesium By similarity
	Binding site	180	1	FMN By similarity
	Binding site	188	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8U262 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: F2AB341CB6E2070A	FASTA	212	23,813
	10 20 30 40 50 60 MKKSNLDLLI LLAKAGGIEK EILTTSRELS KMLNVSPQTI VRWLEDLEKD GLIKKSESRK 70 80 90 100 110 120 GTLVTITEEG VKFLEKLHEE LSDALYRGII IGEVVSGIGE GAYYVRQYAP LIREYLGFDP 130 140 150 160 170 180 YPGTLNVRVI FPKTIFDALC NVRPIIIPGF TKEGRTFGDV RAYRVKIDNI EGAIVIPSRT 190 200 210 IHPPKIAEII APVNLRKTLN LKDGDRIRIK TL

References

[1]

"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009950 Genomic DNA. Translation: AAL81112.1.
RefSeq	NP_578717.1. NC_003413.1.
3D structure databases
ProteinModelPortal	Q8U262.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000006014; EBPYRP00000005875; EBPYRG00000006013.
GeneID	1468853.
GenomeReviews	Gene locus PF0988 in contig AE009950_GR.
KEGG	pfu:PF0988.
NMPDR	fig|186497.1.peg.1024.
Phylogenomic databases
GeneTree	EBGT00050000023005.
HOGENOM	HBG553349.
OMA	GRHYISL.
ProtClustDB	CLSK253495.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR000835. Tscrpt_reg_HTH_MarR-typ. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. PF01047. MarR. 1 hit. [Graphical view]
PROSITE	PS50995. HTH_MARR_2. 1 hit. Uncertain. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_PYRFU

Accession

Primary (citable) accession number: Q8U262

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program