Reviewed, UniProtKB/Swiss-Prot Q8U262 (RIFK_PYRFU)

Names and origin

Protein names

Recommended name:
    Riboflavin kinase
      Short name=RFK
    EC=2.7.1.161
Alternative name(s):
    CTP:riboflavin 5'-phosphotransferase
    CTP-dependent riboflavin kinase
    Flavokinase

Gene names

Name:	rfk
Ordered Locus Names:	PF0988

Organism

Pyrococcus furiosus [Complete proteome] [HAMAP]

Taxonomic identifier

2261 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	FMN biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP riboflavin kinase activity Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Riboflavin kinase HAMAP MF_01285		PRO_0000322102
Regions
	Nucleotide binding	97 – 102	6	CDP By similarity
	Nucleotide binding	193 – 196	4	CDP By similarity
	Region	1 – 87	87	H-T-H motif-like HAMAP MF_01285
	Region	88 – 212	125	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	124	1	Magnesium By similarity
	Metal binding	126	1	Magnesium By similarity
	Binding site	180	1	FMN By similarity
	Binding site	188	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8U262-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: F2AB341CB6E2070A	FASTA	212	23,813
	10 20 30 40 50 60 MKKSNLDLLI LLAKAGGIEK EILTTSRELS KMLNVSPQTI VRWLEDLEKD GLIKKSESRK 70 80 90 100 110 120 GTLVTITEEG VKFLEKLHEE LSDALYRGII IGEVVSGIGE GAYYVRQYAP LIREYLGFDP 130 140 150 160 170 180 YPGTLNVRVI FPKTIFDALC NVRPIIIPGF TKEGRTFGDV RAYRVKIDNI EGAIVIPSRT 190 200 210 IHPPKIAEII APVNLRKTLN LKDGDRIRIK TL

References

[1]	"The complete sequence of the Pyrococcus furiosus genome." Weiss R.B., Dunn D.M., Robb F.T., Brown J.R. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009950 Genomic DNA. Translation: AAL81112.1.
RefSeq	NP_578717.1.
3D structure databases
SMR	Q8U262. Positions 11-199.
ModBase	Search...
Genome annotation databases
GeneID	1468853.
GenomeReviews	Gene locus PF0988 in contig AE009950_GR.
KEGG	pfu:PF0988.
NMPDR	fig|186497.1.peg.1024.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG553349.
OMA	GAYYVRQ.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR000835. HTH_MarR. IPR002834. Riboflavin_kinase_arc. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. PF01047. MarR. 1 hit. [Graphical view]
PROSITE	PS50995. HTH_MARR_2. 1 hit. Uncertain. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_PYRFU

Accession

Primary (citable) accession number: Q8U262

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 1, 2002
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)