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Q8U260 (SYFB_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phenylalanine--tRNA ligase beta subunit

EC=6.1.1.20
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name=PheRS
Gene names
Name:pheT
Ordered Locus Names:PF0990
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00284

Subunit structure

Tetramer of two alpha and two beta subunits By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00284.

Sequence similarities

Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.

Contains 1 B5 domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processphenylalanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phenylalanine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Phenylalanine--tRNA ligase beta subunit HAMAP-Rule MF_00284
PRO_0000127008

Regions

Domain278 – 35477B5

Sequences

Sequence LengthMass (Da)Tools
Q8U260 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: CFFB32F60D001432

FASTA55663,963
        10         20         30         40         50         60 
MPKFDVAKSD LERLVGREFS VEEWEDLVLY AKCELDDVWE EDGKIYFKLD SKDTNRPDLW 

        70         80         90        100        110        120 
SAEGVARQIR WALGLAKGLP KYEVEESDVV VYVDKKLRNI RPYGVYAVVE GLKLDEEALS 

       130        140        150        160        170        180 
QLIQLQEKVA LTYGRRRREV AIGIFDFDKV KPPIYYRAAE KTEKFVPLGY SEEMTLEEIL 

       190        200        210        220        230        240 
EKHEKGREYG HLIRDKPYYP LLVDSEGNVL SMPPIINSEL TGRVTTETKN VFVDVTGWDL 

       250        260        270        280        290        300 
RKVMLALNVI VTALAERGGK IKRVKVIYPD FEITTPDLTP KEFEVSFEYI RKLSGLELSN 

       310        320        330        340        350        360 
EEIKELLERM MYEVEILSEN KAKVKYPAFR DDIMHTRDVL EDVLIAYGYN NIDPEEPKLA 

       370        380        390        400        410        420 
VQGRGDPFKD FEDAIRDLMV GFGLQEVMTF NLTSKEVQFD KMNIPEEEIV EIANPISSRW 

       430        440        450        460        470        480 
SALRKWLLPS LMEFLSNNTH EEYPQRIFEV GLATLIDESR ETKTVSEPKL AVALAGSGYT 

       490        500        510        520        530        540 
FTNAKEILDS LMRHLGIEYD IEETVHGSFI PGRVGKILVD GKEIGIIGEI HPQVLENWNI 

       550 
QVPVVAFEIF LKPLYR 

« Hide

References

[1]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009950 Genomic DNA. Translation: AAL81114.1.
RefSeqNP_578719.1. NC_003413.1.

3D structure databases

ProteinModelPortalQ8U260.
SMRQ8U260. Positions 2-349.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8U260. 1 interaction.
STRING186497.PF0990.

Proteomic databases

PRIDEQ8U260.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL81114; AAL81114; PF0990.
GeneID1468855.
KEGGpfu:PF0990.

Phylogenomic databases

eggNOGCOG0072.
HOGENOMHOG000105094.
KOK01890.
OMANTHRSYP.

Family and domain databases

Gene3D3.30.56.20. 1 hit.
HAMAPMF_00284. Phe_tRNA_synth_beta2.
InterProIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR004531. Phe-tRNA-synth_IIc_bsu_arc.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR022918. Phe_tRNA_ligase_beta2_bac/arc.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
SMARTSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
[Graphical view]
SUPFAMSSF46955. SSF46955. 2 hits.
SSF56037. SSF56037. 1 hit.
TIGRFAMsTIGR00471. pheT_arch. 1 hit.
PROSITEPS51483. B5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYFB_PYRFU
AccessionPrimary (citable) accession number: Q8U260
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries